Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study

Authors

  • Patricio Vildósola Grez University of Chile
  • Patricia Palma Fluxa University of Chile
  • Paula Zuñiga Saavedra University of Chile
  • Eduardo Fernandez Godoy University of Chile
  • Osmir Batista de Oliveira Junior Universidade Estadual Paulista
  • Gustavo Moncada Cortés University of Chile

DOI:

https://doi.org/10.20396/bjos.v12i4.8640941

Keywords:

Streptococcus mutans, Dental caries, Dental restoration

Abstract

Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge’s criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.

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Author Biographies

Patricio Vildósola Grez, University of Chile

Department of Restorative Dentistry, Operative Dentistry, Dental School, University of Chile, Santiago, Chile.

Patricia Palma Fluxa, University of Chile

Department of Medicine and Oral Pathology, Microbiology Dental School, University of Chile, Santiago, Chile

Paula Zuñiga Saavedra, University of Chile

Department of Restorative Dentistry, Operative Dentistry, Dental School, University of Chile, Santiago, Chile.

Eduardo Fernandez Godoy, University of Chile

Department of Restorative Dentistry, Operative Dentistry, Dental School, University of Chile, Santiago, Chile.

Osmir Batista de Oliveira Junior, Universidade Estadual Paulista

Department of Restorative Dentistry, Araraquara Dental School, UNESP – Univ Estadual Paulista, Araraquara, SP.

Gustavo Moncada Cortés, University of Chile

Department of Restorative Dentistry, Operative Dentistry, Dental School, University of Chile, Santiago, Chile.

Published

2015-10-15

How to Cite

1.
Grez PV, Fluxa PP, Saavedra PZ, Godoy EF, Oliveira Junior OB de, Cortés GM. Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study. Braz. J. Oral Sci. [Internet]. 2015 Oct. 15 [cited 2022 Aug. 14];12(4):292-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941

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