Effect of storage and disinfection methods of extracted bovine teeth on bond strength to dentin


  • Maria Malerba Colombi Humel Piracicaba School of Dentistry
  • Marcelo Tavares Oliveira Piracicaba School of Dentistry
  • Vanessa Cavalli Piracicaba School of Dentistry
  • Marcelo Giannini Piracicaba School of Dentistry http://orcid.org/0000-0003-0443-0115




Dentin. Disinfection. Sterilization. Dentin-bonding agents


The aim of this study was to evaluate the effect of storage and disinfection methods (SDM) on bond strength (BS) to bovine dentin, using two adhesive systems: Adper Single Bond and Clearfil Protect Bond. Method: Extracted bovine teeth were assigned to the following SDM: 100% Humidity (HU); Gamma Radiation (GR); Autoclave (AU); 0.10g/mL Thymol (TH); 10% Formalin (FO); Frozen (FR); 0.2% Sodium Azide (SA) and 0.5% Chloramine T (CT) (n=10). The GR and AU groups were submitted to sterilization methods and stored at 100% humidity for 24 hours at 37C°, before testing. TH, FO, FR, SA and CT groups were stored for three months at 5C°, except for FR (-4C°). The adhesive systems were applied according to manufacturer’s instructions. For BS testing, the micro-shear test was performed, using Tygon mold (0.75mm diameter and 1mm high), which was filled with Clearfil AP-X (Kuraray) composite resin. Data were analyzed by two-way ANOVA and Tukey Test (p<0.05). BS of adhesive systems was similar for all SDM, except for 10% formalin. HU, GR, AU, TH and SA did not change the BS for the adhesives tested. For most of SDM, BS of adhesive systems was similar. Some 3 month-SDM groups (FR, FO and CT) reduced the BS.


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Author Biographies

Maria Malerba Colombi Humel, Piracicaba School of Dentistry

Possui Graduação em Odontologia pela Faculdade de Odontologia de Piracicaba (FOP-UNICAMP), mestrado e Doutorado na área de Dentística, Departamento de Odontologia Restauradora pela Faculdade de Odontologia de Piracicaba(FOP-UNICAMP). Possui trabalhos voltados para a área de adesivos dentários com ênfase em seu comportamento biomecânico e químico, atuando principalmente nos seguintes temas: grau de conversão, resistência de união, sistemas adesivos e comportamento clínico dos materiais odontológicos. Atua em consultório particular como especialista em Dentística e Oclusão.

Marcelo Tavares Oliveira, Piracicaba School of Dentistry

Possui graduação em Odontologia pela Universidade Estadual de Campinas (2000), especialização em Dentística (2002) e Endodontia (2003) pela Universidade Estadual de Campinas, Mestrado em Clínica Odontológica (Área de Concentração em Dentística) pela Universidade Estadual de Campinas (2005). 

Vanessa Cavalli, Piracicaba School of Dentistry

Possui graduação em Odontologia pela Universidade Estadual de Campinas (1999). É Mestre, Especialista e Doutora e Pós-Doutora em Clínica Odontológica - com concentração em Dentística.

Marcelo Giannini, Piracicaba School of Dentistry

Graduação em Odontologia (1992), Mestrado (1997) e Doutorado (1999) em Clínica Odontológica (Área de Dentística) pela Universidade Estadual de Campinas. No ano de 2004, concluiu a Livre Docência e atualmente ocupa o cargo de Professor Associado III no Departamento de Odontologia Restauradora (Área de Dentística) da Faculdade de Odontologia de Piracicaba - Universidade Estadual de Campinas, onde iniciou suas atividades como docente em 1995.


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How to Cite

Humel MMC, Oliveira MT, Cavalli V, Giannini M. Effect of storage and disinfection methods of extracted bovine teeth on bond strength to dentin. Braz. J. Oral Sci. [Internet]. 2016 Jan. 21 [cited 2022 Dec. 5];6(22):1402-6. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643000




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