Effect of storage and disinfection methods of extracted bovine teeth on bond strength to dentin

Authors

  • Maria Malerba Colombi Humel Piracicaba School of Dentistry
  • Marcelo Tavares Oliveira Piracicaba School of Dentistry
  • Vanessa Cavalli Piracicaba School of Dentistry
  • Marcelo Giannini Piracicaba School of Dentistry http://orcid.org/0000-0003-0443-0115

DOI:

https://doi.org/10.20396/bjos.v6i22.8643000

Keywords:

Dentin. Disinfection. Sterilization. Dentin-bonding agents

Abstract

The aim of this study was to evaluate the effect of storage and disinfection methods (SDM) on bond strength (BS) to bovine dentin, using two adhesive systems: Adper Single Bond and Clearfil Protect Bond. Method: Extracted bovine teeth were assigned to the following SDM: 100% Humidity (HU); Gamma Radiation (GR); Autoclave (AU); 0.10g/mL Thymol (TH); 10% Formalin (FO); Frozen (FR); 0.2% Sodium Azide (SA) and 0.5% Chloramine T (CT) (n=10). The GR and AU groups were submitted to sterilization methods and stored at 100% humidity for 24 hours at 37C°, before testing. TH, FO, FR, SA and CT groups were stored for three months at 5C°, except for FR (-4C°). The adhesive systems were applied according to manufacturer’s instructions. For BS testing, the micro-shear test was performed, using Tygon mold (0.75mm diameter and 1mm high), which was filled with Clearfil AP-X (Kuraray) composite resin. Data were analyzed by two-way ANOVA and Tukey Test (p<0.05). BS of adhesive systems was similar for all SDM, except for 10% formalin. HU, GR, AU, TH and SA did not change the BS for the adhesives tested. For most of SDM, BS of adhesive systems was similar. Some 3 month-SDM groups (FR, FO and CT) reduced the BS.

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Author Biographies

Maria Malerba Colombi Humel, Piracicaba School of Dentistry

Possui Graduação em Odontologia pela Faculdade de Odontologia de Piracicaba (FOP-UNICAMP), mestrado e Doutorado na área de Dentística, Departamento de Odontologia Restauradora pela Faculdade de Odontologia de Piracicaba(FOP-UNICAMP). Possui trabalhos voltados para a área de adesivos dentários com ênfase em seu comportamento biomecânico e químico, atuando principalmente nos seguintes temas: grau de conversão, resistência de união, sistemas adesivos e comportamento clínico dos materiais odontológicos. Atua em consultório particular como especialista em Dentística e Oclusão.

Marcelo Tavares Oliveira, Piracicaba School of Dentistry

Possui graduação em Odontologia pela Universidade Estadual de Campinas (2000), especialização em Dentística (2002) e Endodontia (2003) pela Universidade Estadual de Campinas, Mestrado em Clínica Odontológica (Área de Concentração em Dentística) pela Universidade Estadual de Campinas (2005). 

Vanessa Cavalli, Piracicaba School of Dentistry

Possui graduação em Odontologia pela Universidade Estadual de Campinas (1999). É Mestre, Especialista e Doutora e Pós-Doutora em Clínica Odontológica - com concentração em Dentística.

Marcelo Giannini, Piracicaba School of Dentistry

Graduação em Odontologia (1992), Mestrado (1997) e Doutorado (1999) em Clínica Odontológica (Área de Dentística) pela Universidade Estadual de Campinas. No ano de 2004, concluiu a Livre Docência e atualmente ocupa o cargo de Professor Associado III no Departamento de Odontologia Restauradora (Área de Dentística) da Faculdade de Odontologia de Piracicaba - Universidade Estadual de Campinas, onde iniciou suas atividades como docente em 1995.

References

Pagniano RP, Scheid RC, Rosen S, Beck FM. Reducing airborne microbes in the preclinical dental laboratory. J Dent Educ. 1986; 50: 234-5.

Rueggeberg FA. Substrate for adhesion testing to tooth structure - review of the literature. Dent Mater. 1991; 7: 2-10.

Pashley DH, Sano H, Ciucchi B, Yoshiyama M, Carvalho RM. Adhesion testing of dentin bonding agents: a review. Dent Mater. 1995; 11: 117-25.

Strawn SE, White JM, Marshall GW, Gee L, Goodis HE, Marshall SJ. Spectroscopic changes in human dentine exposed to various storage solutions—short term. J Dent. 1996; 24: 417-23.

DeWald JP. The use of extracted teeth for in vitro bonding studies: a review of infection control considerations. Dent Mater. 1997; 13: 74-81.

Dominici JT, Eleazer PD, Clark SJ, Staat RH, Scheetz JP. Disinfection/sterilization of extracted teeth for dental student use. J Dent Educ. 2001; 65: 1278-80.

Watanabe I, Nakabayashi N. Measurement methods for adhesion to dentine: the current status in Japan. J Dent. 1994; 22: 67-72.

McGuckin RS, Pashley DH. Effect of disinfection/sterilization treatments on Gluma-mediated dentin shear bond strengths. Am J Dent. 1990; 3: 278-82.

White JM, Goodis HE, Marshall SJ, Marshall GW. Sterilization of teeth by gamma radiation. J Dent Res. 1994; 73: 1560-7.

Soares LE, Brugnera Junior A, Zanin FA, Pacheco MT, Martin AA. Effects of treatment for manipulation of teeth and Er:YAG laser irradiation on dentin: a Raman spectroscopy analysis. Photomed Laser Surg. 2007; 25: 50-7.

Titley KC, Chernecky R, Rossouw PE, Kulkarni GV. The effect of various storage methods and media on shear-bond strengths of dental composite resin to bovine dentine. Arch Oral Biol. 1998; 43: 305-11.

Nakabayashi N, Takarada K. Effect of HEMA on bonding to dentin. Dent Mater. 1992; 8: 125-30.

De Goes MF, Giannini M, Foxton RM, Nikaido T, Tagami J. Bond strength of adhesive systems to coronal and root dentin. J Prosthet Dent. in press.

Cheung DT, Perelman N, Tong D, Nimni ME. The effect of gamma-irradiation on collagen molecules, isolated alpha-chains, and crosslinked native fibers. J Biomed Mater Res. 1990; 24: 581-9.

Watanabe LG, Marshall GW, Jr., Marshall SJ. Dentin shear strength: effects of tubule orientation and intratooth location. Dent Mater. 1996; 12: 109-15.

Habelitz S, Marshall GW, Jr., Balooch M, Marshall SJ. Nanoindentation and storage of teeth. J Biomech. 2002; 35: 995-8.

Goodis HE, Marshall GW Jr, White JM, Gee L, Hornberger B, Marshall SJ. Storage effects on dentin permeability and shear bond strengths. Dent Mater. 1993; 9: 79-84.

Miranda WG, Placido E, Moura SK, Cardoso PE. Influence of postextraction substrate aging on the microtensile bond strength of a dental adhesive system. J Adhes Dent. 2005; 7: 193-6.

Haller B, Hofmann N, Klaiber B, Bloching U. Effect of storage media on microleakage of five dentin bonding agents. Dent Mater. 1993; 9: 191-7.

Giannini M, Soares CJ, de Carvalho RM. Ultimate tensile strength of tooth structures. Dent Mater. 2004; 20: 322-9.

de Menezes MJ, Arrais CA, Giannini M. Influence of lightactivated and auto- and dual-polymerizing adhesive systems on bond strength of indirect composite resin to dentin. J Prosthet Dent. 2006; 96: 115-21.

Retief DH, Wendt SL, Bradley EL, Denys FR. The effect of storage media and duration of storage of extracted teeth on the shear bond strength of Scotchbond 2/Silux to dentin. Am J Dent. 1989; 2: 269-73.

Cavalli V, Reis AF, Giannini M, Ambrosano GM. The effect of elapsed time following bleaching on enamel bond strength of resin composite. Oper Dent. 2001; 26: 597-602.

Arrais CA, Micheloni CD, Giannini M, Chan DC. Occluding effect of dentifrices on dentinal tubules. J Dent. 2003; 31: 577-84.

Koutsi V, Noonan RG, Horner JA, Simpson MD, Matthews WG, Pashley DH. The effect of dentin depth on the permeability and ultrastructure of primary molars. Pediatr Dent. 1994; 16: 29-35.

McGuckin RS, Powers JM, Li L. Bond strengths of dentinal bonding systems to enamel and dentin. Quintessence Int. 1994; 25: 791-6.

Sano H, Ciucchi B, Matthews WG, Pashley DH. Tensile properties of mineralized and demineralized human and bovine dentin. J Dent Res. 1994; 73: 1205-11.

Sano H, Takatsu T, Ciucchi B, Horner JA, Matthews WG, Pashley DH. Nanoleakage: leakage within the hybrid layer. Oper Dent. 1995; 20: 18-25.

Carvalho RM, Yoshiyama M, Brewer PD, Pashley DH.

Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy. Arch Oral Biol.

; 41: 379-86.

Gwinnett AJ. Dentin bond strength after air drying and rewetting. Am J Dent. 1994; 7: 144-8.

Lee SY, Lin CT. Storage effect on dentine structure and on resultant composite bond strengths. J Oral Rehabil. 1997; 24: 823-34.

Prati C, Ferrieri P, Galloni C, Mongiorgi R, Davidson CL. Dentine permeability and bond quality as affected by new bonding systems. J Dent. 1995; 23: 217-26.

Shaffer SE, Barkmeier WW, Gwinnett AJ. Effect of disinfection/ sterilization on in-vitro enamel bonding. J Dent Educ. 1985; 49: 658-9.

Tate WH, White RR. Disinfection of human teeth for educational purposes. J Dent Educ. 1991; 55: 583-5.

Pashley EL, Tao L, Pashley DH. Sterilization of human teeth: its effect on permeability and bond strength. Am J Dent. 1993; 6: 189-91.

Shimada Y, Yamaguchi S, Tagami J. Micro-shear bond strength of dual-cured resin cement to glass ceramics. Dent Mater. 2002; 18: 380-8.

Goodis HE, Marshall GW, Jr., White JM. The effects of storage after extraction of the teeth on human dentine permeability in vitro. Arch Oral Biol. 1991; 36: 561-6.

Cenci MS, Piva E, Potrich F, Formolo E, Demarco FF, Powers JM. Microleakage in bonded amalgam restorations using different adhesive materials. Braz Dent J. 2004; 15: 13-8.

Sterrett JD, Sutow EJ, Murphy HJ. Surface topography of demineralized dentin preceded by formalin fixation. Quintessence Int. 1992; 23: 61-4.

Fujisawa S, Kadoma Y. Effect of phenolic compounds on the polymerization of methyl methacrylate. Dent Mater. 1992; 8: 324-6.

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Published

2016-01-21

How to Cite

1.
Humel MMC, Oliveira MT, Cavalli V, Giannini M. Effect of storage and disinfection methods of extracted bovine teeth on bond strength to dentin. Braz. J. Oral Sci. [Internet]. 2016 Jan. 21 [cited 2022 Dec. 5];6(22):1402-6. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643000

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