Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients

Keywords: Periodontitis, Bacteria, Molecular biology.

Abstract

Aim: The aim of this study was to compare the microbial profile of subgingival sites in Periodontitis (Pd) patients and healthy ones. Methods: Eighteen patients with Pd and 18 gender-matched healthy controls were selected. Subgingival samples were collected from three types of sites: 1) healthy site of healthy subjects (probing pocket depth (PPD) ≤ 3mm, CG), 2) healthy site of Pd patients (PPD ≤ 3mm, PG-C) and 3) diseased site (PPD > 3mm) of the same Pd patients (PG-T). All sites were subjected to microbial analysis for the detection of 40 bacterial species by the "Checkerboard DNA-DNA hybridization" technique. Results: It was observed a great diversity of bacteria in all patients evaluated. The sites from the Pd groups (PG-T and PG-C) showed a higher overall count of the studied bacteria than those of the CG group, especially from Green, Orange, and Red complexes. Also, PG-T showed a higher prevalence of Red complex bacteria than CG. Individual pathogens, such as Porphyromonas gingivalis, Treponema denticola and Treponema socranskii were detected in higher levels and/or prevalence in Pd than in control patients. However, it was not observed any difference between PG-T and PG-C. Conclusion: Pd patients showed higher prevalence and counts of some putative periodontal bacteria, especially from the red complex, than control ones, regardless of the severity of their sites.

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Author Biographies

Carlos Eduardo Barros, Veiga de Almeida University

Department of Periodontology, School of Dentistry - Veiga de Almeida University.

Vivian Siqueira, Veiga de Almeida University

Department of Periodontology, School of Dentistry - Veiga de Almeida University.

Dennis Carvalho, Veiga de Almeida University
Department of Periodontology, School of Dentistry, Veiga de Almeida University (UVA), Brazil.
Antonio Canabarro, Veiga de Almeida University

Department of Periodontology, School of Dentistry - Veiga de Almeida University.

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Published
2019-11-12
How to Cite
Barros, C. E., Siqueira, V., Carvalho, D., & Canabarro, A. (2019). Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients. Brazilian Journal of Oral Sciences, 18, e191417. https://doi.org/10.20396/bjos.v18i0.8657248