Keywords
Stem cells
Transforming growth factor beta
Extracellular matrix proteins
How to Cite
Funding data
-
Fundação de Amparo à Pesquisa do Estado de São Paulo
Grant numbers 2018/20316-6 -
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Abstract
Aim: This study evaluated the effect of different concentrations of transforming growth factor beta 1 (TGF-β1) on stem cells from human exfoliated deciduous teeth (SHED) viability, proliferation, migration and differentiation into odontoblasts. Methods: SHED was treated with different concentrations of TGF-β1 (1.0, 5.0 and 10.0 ng/mL). Sulforhodamine B and MTT assays evaluated the cell proliferation and viability at 1, 3, 5, and 7 days and Migration assay at 24h. RT-PCR verified Dentin matrix protein1 (DMP-1) and Dentin Sialophosphoprotein (DSPP) mRNA expression for 1, 7 and 14 days. The data were analyzed by one-way and two-way ANOVA, followed by Tukey test (p<0.05). Results: All tested TGF-β1 concentrations increased SHED proliferation compared with the negative control (untreated), from day 3 of treatment (p=0.000), with no loss of cell viability. Cell migration was higher in media containing TGF-β1 (1.0-10.0 ng/mL) than in negative and positive control media, with 10 or 20% Fetal Bovine Serum, respectively (p=0.000). Treatment with TGF-β1 for up to 14 days induced the expression of the odontoblast markers DMP- 1 and DSPP. DMP-1 expression was intense early after treatment with 10.0 ng/mL TGF-β1 and increased progressively from days 1-14 of treatment with 1.0 and 5.0 ng/mL TGF-β1. In contrast, DSPP expression was detected after 14 days of treatment with 10.0 ng/mL TGF-β1. Conclusion: Different concentrations of TGF-β1 on SHED promoted positive effect on proliferation and migration, with no loss of cell viability. The concentrations of 10.0 ng/mL TGF-β1 for 14 days induced the expression of the odontoblast markers DMP-1 and DSPP.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2025 Ana Paula Fernandes, Leandro Borges Araújo, Fábio Antonio Colombo, Eloá Cristina Passucci Ambrosio, Ana Beatriz Vieira da Silveira, Mariel Tavares Oliveira Prado Bergamo, Paula Karine Jorge, Maria Aparecida Andrade Moreira Machado, Thais Marchini Oliveira, Vivien Thiemy Sakai
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