@article{Câmara_Albuquerque_Aguiar_Correia_2015, place={Limeira, SP}, title={Antimicrobial activity of chlorhexidine in root canals instrumented with the ProTaper Universal™ System}, volume={9}, url={https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641838}, DOI={10.20396/bjos.v9i3.8641838}, abstractNote={Aim: The purpose of the present study was to evaluate the antimicrobial activity of 0.2%, 1%, and 2% chlorhexidine in root canals instrumented with the ProTaper Universal™ system. Methods: Fifty human mandibular premolar teeth were infected with a mixture of Candida albicans, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus. The specimens were randomly divided into 5 groups with 10 root canals according to the irrigant used. All root canals were instrumented with the ProTaper Universal™ system. Assessment of the antimicrobial action of the irrigant was performed before, during, and after instrumentation. Data were analyzed statistically by Chi-squared test and the Fisher exact test at 5% significance level. Results: The 0.2% chlorhexidine solution was ineffective against all test microorganisms. The 1% chlorhexidine solution was effective in eliminating P. aeruginosa and C. albicans after the use of the F1 and F3 instruments, respectively. The 2% chlorhexidine solution was effective at killing S. aureus, P. aeruginosa and C. albicans after the use of the S1 instrument. There were statistically significant differences (p<0.05) between the concentrations of chlorhexidine and the instruments used. Conclusions: The 0.2% chlorhexidine solution in combination with rotary instrumentation was ineffective against all test microorganisms. The 1% chlorhexidine solution was ineffective against S. aureus and E. faecalis. The 2% chlorhexidine solution was not sufficient to inactivate E. faecalis.}, number={3}, journal={Brazilian Journal of Oral Sciences}, author={Câmara, Andréa Cruz and Albuquerque, Miracy Muniz de and Aguiar, Carlos Menezes and Correia, Ana Cristina Regis de Barros}, year={2015}, month={Nov.}, pages={402–409} }