TY - JOUR AU - Ercolin, Ludmila Tavares Costa AU - Ambrosano, Gláucia Maria Bovi AU - Probst, Livia Fernandes AU - Ribeiro-Dasilva, Margarete C. AU - Tomar, Scott L. AU - Possobon, Rosana de Fátima PY - 2016/10/13 Y2 - 2024/03/29 TI - Salivary cortisol level and uncooperative behavior in pediatric dental practice JF - Brazilian Journal of Oral Sciences JA - Braz. J. Oral Sci. VL - 15 IS - 1 SE - Article DO - 10.20396/bjos.v15i1.8647125 UR - https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8647125 SP - 57-61 AB - <p class="Pa7"><span>Aim: To investigate the relation between uncooperative behavior and salivary cortisol level in children who underwent preventive dental care. Methods: The sample was composed by 10 children of both sexes aged 40 to 52 months, presenting uncooperative behavior during dental preventive treatments. The saliva collection was performed using a cotton wheel and an Eppendorf tube (Sarstedt Salivete®) in 3 different moments: a) at home, on a day without dental treatment and at the same time on the day of the sessions treatment; b) 30 min after the end of the session, when there was manifestation of uncooperative behavior; c) 30 min after the end of the session, when there was a cooperative behavior of the child. A sample of saliva was centrifuged for 5 minutes at 2400 rpm, 1 of mL of saliva was pipetted in an Eppendorf tube and stored in a freezer at -20 ° C. For the determination of the levels of salivary cortisol was used an Active® kit for cortisol enzyme immunoassay (EIA) DSL-10-67100, composed of specific rabbit antibody anti-cortisol. Data were analyzed statistically for the uncooperative behavior issued in the beginning and at the end of sessions, using the paired t test (p&lt;0.05) and for cortisol levels in saliva samples at home, after the beginning and at the end of sessions, using repeated-measures ANOVA and Tukey’s test (p&lt;0.05). Results: During expression of uncooperative behavior in preventive dental care sessions the salivary cortisol level was significantly higher (0.65 ± 0.25 μg/dL) compared with expression of collaborative behavior (0.24 ± 0.10 μg/dL). Conclusions: It is possible to conclude that, even under preventive.</span></p> ER -