Brazilian Journal of Oral Sciences https://periodicos.sbu.unicamp.br/ojs/index.php/bjos <p><strong>Scope</strong>: The Brazilian Journal of Oral Sciences, published by the Piracicaba Dental School – UNICAMP, is an international non-profit <span lang="EN-US">open-access </span>online journal. The primary goal of the Brazilian Journal of Oral Sciences is to publish the outcomes of original investigations as well as invited reviews, case reports, short communications, and letters to the editor in the field of Dentistry and related disciplines. The Brazilian Journal of Oral Sciences is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels.<br /><strong>Qualis</strong>:B1<br /><strong>Knowledge Area</strong>: Health Sciences<br /><strong>Foundation</strong>: 2002<br /><strong>Short title</strong>: Braz. J. Oral. Sci.<br /><strong>e-ISSN</strong>:1677-3225<br /><strong>E-mail</strong>: <a href="https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/management/settings/context/mailto:brjorals@fop.unicamp.br">brjorals@fop.unicamp.br</a><br /><strong>Faculty</strong>: <a title="FOP" href="http://www.fop.unicamp.br/index.php/pt-br/" target="_blank" rel="noopener">FOP</a><br /><strong>UNICAMP DOI Prefix</strong>: 10.20396<br /><a title="CC-BY" href="https://creativecommons.org/licenses/by/4.0/" target="_blank" rel="noopener"><img src="https://i.creativecommons.org/l/by/4.0/80x15.png" alt="Licença Creative Commons" /></a></p> Universidade Estadual de Campinas en-US Brazilian Journal of Oral Sciences 1677-3225 <p>The <strong>Brazilian Journal of Oral Sciences</strong> uses the <a href="https://creativecommons.org/licenses/by/4.0" target="_blank" rel="noopener">Creative Commons license (CC)</a>, thus preserving the integrity of the articles in an open access environment.</p> Prevalence and influence of dental development anomalies in smile esthetics https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667434 <p>Aim: To determine the prevalence of dental development anomalies and type of influence on the smile of adolescent students. Method: This was a cross-sectional and analytical study carried out in two public (A1) and two private (A2) schools chosen by lot in the city of Parnaíba-Piauí. The sample calculation was based on the target population: number of people enrolled in public and private schools between 15 and 19 years, in the city of Parnaíba-PI, which totaled 6209 students in 2020, according to a survey carried out by the Brazilian Institute of Geography and Statistics – IBGE. A questionnaire on epidemiological data and aesthetic self-perception of the smile was applied to 160 adolescents between 15 and 19 years old, from August 2020 to July 2021. The clinical examination was carried out under natural light, to check for the presence of anomaly(s) in the dental development. Students who presented only one pathology would be called group 1 (G1), those who presented two would be called group 2 (G2) and those who presented 3 or more would be called group 3 (G3). On the other hand, adolescents in whom no anomaly was evidenced would participate in the control group (CG), both in A1 and A2. Results: It was observed that 37.5% of the sample had only a type of dental anomaly, corresponding to 60 individuals. The most prevalent were enamel hypoplasia, fusion, transposition, agenesis, ectopic eruption, microdent and dens-in-dent. It was possible to verify a higher prevalence in the maxilla, private schools (76.6%) and females (86.6%). In 45% of adolescents with dental anomalies, embarrassment was observed when smiling. Conclusion: The prevalence was relatively high, highlighting the enamel hypoplasia, influencing the smile esthetics of a reasonable number of adolescents, whether for acquaintances, strangers or even for photographs.</p> Marcelo Lucio Sousa Silva Junior Millena Lopes de Brito Breno Wesley Leal Carvalho Elen Maria Carvalho da Silva Ana de Lourdes Sá de Lira Copyright (c) 2022 Marcelo Lucio Sousa Silva Junior, Millena Lopes de Brito, Breno Wesley Leal Carvalho, Elen Maria Carvalho da Silva, Ana de Lourdes Sá de Lira https://creativecommons.org/licenses/by/4.0 2022-09-06 2022-09-06 22 e237434 e237434 10.20396/bjos.v22i00.8667434 Use of scanning electron microscope to evaluate the marginal fit of protocol bars obtained through benchtop or intraoral digital scanners https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8669079 <p>Aim: To evaluate the marginal fit of protocol bars milled from digital models obtained by conventional molding followed by bench scanning or digital molding with an intraoral scanner. Methods: Four morse-cone implants and the mini-pillars were installed in a 3D printed mandible model (master model). Digital models of the master model were obtained by (n=10): (Group A - Conventional) conventional (analog) molding of the master model followed by bench scanning or (Group B - Digital) molding of the master model with an intraoral scanner. All-on-four protocol bars were designed and milled from the digital models for both groups and screwed into the master model. Scanning electron microscopy (SEM) images from the distal, central, and mesial regions of each implant were obtained and the implant-protocol bar marginal fit was measured in an image software (Image J). The mean misfit of each region was analyzed by two-factor ANOVA, Tukey test, and Student’s t-test (0,05 = 0.05). Results: The digital approach (B) showed higher misadaptation than the conventional approach (A, p &lt; 0.05), regardless of the region evaluated. In group A, the central region showed higher maladjustment than the mesial region (p&lt;0.05), however, there were no differences among regions of group B (p&gt;0.05). Conclusion: The conventional method of acquiring digital models using the bench scanner produced bars for the All-On-Four protocol with better marginal fit than the digital models obtained with an intraoral scanner.</p> Renes Augusto Parizotto Vanessa Cavalli Rafael Lacerda Zandoná Geraldo Alberto Pinheiro de Carvalho Aline Batista Gonçalves Franco Elimario Venturin Ramos Sérgio Candido Dias Copyright (c) 2022 Renes Augusto Parizotto, Vanessa Cavalli, Rafael Lacerda Zandoná, Geraldo Alberto Pinheiro de Carvalho, Aline Batista Gonçalves Franco , Elimario Venturin Ramos, Sérgio Candido Dias https://creativecommons.org/licenses/by/4.0 2022-12-19 2022-12-19 22 e239079 e239079 10.20396/bjos.v22i00.8669079 Assessment of salivary opiorphin in oral lichen planus https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667270 <p>Opiorphin is a pentapeptide, which could be isolated from human fluids and has a decreasing effect on pain. Aim: Since lichen planus is a chronic mucocutaneous disease, which causes pain or burning feeling in the oral mucosa, this study aimed to compare salivary opiorphin levels of oral lichen planus (OLP) patients with healthy subjects. Methods: This case-control study, was performed on 24 patients with OLP lesions and 21 healthy subjects. After collecting unstimulated saliva, opiorphin levels were compared between two groups through statistical analyses. Results: There was not any significant difference between OLP patients and healthy subjects according to salivary opiorphin concentration (p=0.378). Also, in the OLP group, opiorphin concentration was not significantly different between males and females (p=0.601). Analytical analysis could not show any remarkable difference between various severity of OLP lesions regarding to salivary opiorphin levels (p=0.653). Conclusion: In this study, salivary opiorphin levels was not significantly different between patients with OLP and healthy subjects; however, more studies are suggested for better assessment of salivary opiorphin levels in various types of OLP lesions and its correlation with pain severity.</p> Zohreh Dalirsani Maryam Amirchaghmaghi Ala Ghazi Seyed Isaac Hashemy Mahboobeh Taherizadeh Copyright (c) 2022 Zohreh Dalirsani, Maryam Amirchaghmaghi, Ala Ghazi, Seyed Isaac Hashemy, Mahboobeh Taherizadeh https://creativecommons.org/licenses/by/4.0 2023-01-26 2023-01-26 22 e237270 e237270 10.20396/bjos.v22i00.8667270 Longitudinal analyses of composite resin restoration on erosive lesions https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8666839 <p>Aim: To evaluate the influence of the biomodification of erosive lesions with a chitosan nanoformulation containing green tea (NanoCsQ) on the clinical performance of a composite resin. Methods: The study was performed in a split-mouth, randomized and double-blinded model with 20 patients with 40 erosive lesions. The patient’s teeth were randomized into two groups (n=20) according to the surface treatment: 1) Without biomodification (control), and 2) Biomodification with NanoCsQ solution (experimental). The lesions were restored with adhesive (Tetric N-bond, Ivoclar) and composite resin (IPS Empress Direct, Ivoclar). The restorations were polished and 7 days (baseline), 6 months, and 12 months later were evaluated according to the United States Public Health Service (USPHS) modified criteria, using clinical exam and photographics. Data were analyzed by Friedman’s and Wilcoxon signed-rank tests. Results: No significant differences were found between the control and experimental groups (p=0.423), and also among the follow-up periods (baseline, six months, and 12 months) (p=0.50). Regarding the retention criteria, 90% of the restoration had an alpha score in the control group. Only 10% of the restorations without biomodification (control) had a score charlie at the 12-month follow-up. None of the patients reported post-operatory sensitivity. Conclusion: The NanoCsQ solution did not negatively affect the performance of the composite resin restorations after 12 months.</p> Renato Gonçalves dos Reis Antônio Cláudio Tedesco Fabiana Almeida Curylofo-Zotti Thiago Vinicius Cortez Hiago Salge Borges Aline Evangelista Souza-Gabriel Silmara Aparecida Milori Corona Copyright (c) 2022 Renato Gonçalves dos Reis, Antônio Cláudio Tedesco, Fabiana Almeida Curylofo-Zotti, Thiago Vinicius Cortez, Hiago Salge Borges, Aline Evangelista Souza-Gabriel, Silmara Aparecida Milori Corona https://creativecommons.org/licenses/by/4.0 2022-11-09 2022-11-09 22 e236839 e236839 10.20396/bjos.v22i00.8666839 Higher maternal age is associated with higher occurrence of cleft lip/palate in neonates under intensive care https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8669246 <p>Aim: To assess the prevalence of cleft lip and/or cleft palate (CL/P) and associated variables in neonates admitted to neonatal intensive care units (ICU). Methods: Medical charts for neonates born and admitted to the ICU between 2012 and 2018 were reviewed. Obstetric and neonatal variables were collected by a trained researcher. In the case group, all neonates with CL/P were included. The control group was formed by matching sex, prematurity and month of birth using random number generation. Neonates with congenital malformations were excluded from the control group. Adjusted logistic regression was used (p&lt;0.05). Results: The prevalence of CL/P was 0.43% (n=15). Five cases were excluded, as pairing was not possible. Twenty neonates were included in the control group. In the final multivariate model, CL/P was only associated with increased maternal age. For each year of increase in maternal age, neonates had a 35.2% higher chance of presenting CL/P (95% confidence interval: 1.021–1.792). Conclusions: Higher maternal age was associated with higher occurrence of CL/P in neonates admitted to the ICU. No other neonatal or maternal independent variables were associated with CL/P. Due to missing data, interpretation of study results must be approached with caution.</p> Luísa de Souza Maurique Francisco Wilker Mustafa Gomes Muniz Nathalia Preissler Vaz Silveira Melissa Camassola Bibiana Mello de Oliveira Copyright (c) 2022 Luísa de Souza Maurique, Francisco Wilker Mustafa Gomes Muniz, Nathalia Preissler Vaz Silveira, Melissa Camassola, Bibiana Mello de Oliveira https://creativecommons.org/licenses/by/4.0 2022-11-03 2022-11-03 22 e239246 e239246 10.20396/bjos.v22i00.8669246 Fracture strength and failure load of CAD/CAM fabricated endocrowns performed with different designs https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667338 <p>Aim: Endocrown restorations are commonly used to rehabilitate endodontically treated posterior teeth and their use is wellfounded in these cases. However, to date, there is little scientific evidence of their behavior in anterior teeth. The aim of this in vitro study was to evaluate the compressive strength of upper central incisors teeth, restored with glass-ceramic total crowns by the conventional anatomical core technique, and compare them to teeth restored with endocrowns with and without the presence of ferrule. Methods: Thirty teeth were randomly distributed into three groups: GE2 - endocrown group with 2 mm ferrule, GE0 - endocrown group without a ferrule, and GC - conventional crown with intraradicular post group. Crowns were cemented and teeth submitted to the 45o compression test until the fracture happened. Fractured specimens were analyzed to determine the fracture pattern. Descriptive analysis of the variables was performed and one-way analysis of variance was utilized to analyze the data for significant differences at p &lt; 0.05. Results: The results of the control group (284.5 ± 201.05N) showed the highest fracture resistance value, followed by the 2mm group (274.54 ± 199.43N) and by the 0mm group (263.81 ± 80.05N). There was no statistically significant difference between all the groups (p = 0.964). Conclusions: The absence of a cervical enamel necklace favored a debonding of the pieces and endodontically treated anterior teeth could be restored with endocrown, which could be considered a conservative and viable treatment option.</p> Fernanda Angeloni de Souza Catarina Borges da Fonseca Cumerlato Pedro Paulo Feltrin Ricardo Tatsuo Inoue Raquel Virginia Zanetti Copyright (c) 2022 Fernanda Angeloni de Souza, Catarina Borges da Fonseca Cumerlato, Pedro Paulo Feltrin, Ricardo Tatsuo Inoue, Raquel Virginia Zanetti https://creativecommons.org/licenses/by/4.0 2022-10-17 2022-10-17 22 e237338 e237338 10.20396/bjos.v22i00.8667338 Effects of black tea tooth staining previously to 35% hydrogen peroxide bleaching https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8668082 <p>Aim: To determine if the artificial staining with black tea (BT) influences the enamel microhardness before in-office bleaching and if BT staining is necessary to evaluate the efficacy of bleaching with 35% hydrogen peroxide Methods: Enamel/dentin blocks were randomized into groups according to the staining protocol (n=5/group): (CO) control – maintained in artificial saliva solution (AS); (BT4) immersed in black tea solution for 4 h; (BT24) immersed in black tea solution for 24 h. After the staining protocols, all specimens were kept in AS for one week, followed by bleaching (three sessions of HP application for 40 min). Knoop surface microhardness (kgF/mm2) was determined at baseline (T0), after staining (T1), after 7 days of storage in AS (T2), and after bleaching (T3). The color (ΔE00) and coordinate changes (ΔL, Δa, Δb) were measured using a digital spectrophotometer at T0 and T3. Data were submitted to one-way (ΔE00, ΔL, Δa, Δb) or two-way ANOVA repeated measures (kgF/mm2) and Tukey’s test (a=5%). Results: The staining protocols (BT4 and BT24) promoted significantly lower microhardness (T1 and T2, p&lt;0.05) than CO, whereas CO was the only group to maintain microhardness values over time. Bleaching promoted perceptible ΔE00 without a significant difference among the groups regardless of the staining protocol (p=0.122). CO and BT4 showed no differences in terms of ΔL and Δa (p&gt;0.05), but BT4 displayed a higher Δb than CO. Conclusion: The artificial staining with BT negatively affected the enamel surface microhardness and was not essential to evaluate the efficacy of 35% hydrogen peroxide bleaching.</p> Samuel da Silva Palandi Matheus Kury Mayara Zaghi Dal Picolo Fernando Luis Esteban Florez Vanessa Cavalli Copyright (c) 2022 Samuel da Silva Palandi, Matheus Kury, Mayara Zaghi Dal Picolo, Fernando Luis Esteban Florez, Vanessa Cavalli https://creativecommons.org/licenses/by/4.0 2022-09-13 2022-09-13 22 e238082 e238082 10.20396/bjos.v22i00.8668082 Bond strength of bulk fill composite to teeth prepared with Er https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8668727 <p>Aim: The present <em>in vitro </em>study aimed to evaluate the bond strength of a bulk fill composite on dentin surfaces prepared with the Er: YAG laser. Methods: Twenty-four permanent third molars were selected and divided into 2 groups: CP - Conventional preparation with high-speed handpiece (control) and LA (laser) - Preparation with Er: YAG laser. The occlusal surface was removed to expose coronal dentin, which was subsequently prepared with a high-speed handpiece or Er: YAG laser (350mJ, 4Hz, 1.5 ml/min water flow). Both groups were restored with Filtek One Bulk Fill (3M ESPE) composite resin. After 24 hours, the samples were evaluated for microtensile bond strength (μTBS), fracture pattern, and scanning electron microscopy (SEM). Results: The data obtained in the μTBS test were submitted to <em>t</em>-test (α=0.05). The results showed no difference in μTBS when the different types of cavity preparation were compared (ρ=0.091). Fracture patterns revealed the prevalence of cohesive fracture in composite resin in CP (83.3%) and adhesive fracture in LA (92.1%). In the SEM analysis, the LA group demonstrated the presence of gaps between the composite resin and the irradiated dentin surface. The hybrid layer exhibited more regularity with the presence of longer and uniform resin tags in the CP group. Conclusion: The type of cavity preparation did not influence the values of bulk fill composite resin μTBS to dentin. Fracture patterns and scanning electron microscopy analyses suggested less interference at the adhesive interface in preparations performed using CP.</p> Marcos Roberto de Lima Benati Jean Carlos Baioni Amanda Guerra Cavalcante de Souza Laura Nobre Ferraz Ana Luisa Botta Martins de Oliveira Rafael Pino Vitti Renata Siqueira Scatolin Copyright (c) 2022 Marcos Roberto de Lima Benati, Jean Carlos Baioni, Amanda Guerra Cavalcante de Souza, Laura Nobre Ferraz, Ana Luisa Botta Martins de Oliveira, Rafael Pino Vitti, Renata Siqueira Scatolin https://creativecommons.org/licenses/by/4.0 2022-12-19 2022-12-19 22 e238727 e238727 10.20396/bjos.v22i00.8668727 Temporomandibular disorders and associated comorbidities among brazilian dental students during COVID-19 https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667397 <p>Aim: This study aimed to assess the prevalence of temporomandibular disorders (TMD) and psychosocial comorbidities in undergraduate dental students in a southern Brazilian university, during the COVID-19 pandemic. Also, it aimed to verify the association between psychosocial factors and TMD. Methods: Fonseca Anamnestic Index, Depression Anxiety Stress Scale (DASS-21), a socioeconomic questionnaire, and questions about academic performance and social distancing were applied. Poisson regression analysis was used to assess the association of predictive variables with TMD. Results: The prevalence of TMD was found to be 82.4%, and more than half of the students had some degree of stress, anxiety, and depression. Students who had symptoms of stress (RR 1.11; 95% CI 1.04-1.19), anxiety (RR 1.19; 95% CI 1.12-1.27) and reported academic performance worsening (RR 1.12; 95% CI 1.07-1.19) had higher TMD scores. Conclusion: The findings suggest that TMD was highly prevalent among dental students at a federal university in southern Brazil during the pandemic, being associated with high levels of stress, anxiety, poor academic performance, and greater social distancing.</p> Francyéllen Teixeira da Silva Jessica Klöckner Knorst Lucas Machado Maracci Vilmar Antônio Ferrazzo Gabriela Salatino Liedke Tatiana Bernardon Silva Mariana Marquezan Copyright (c) 2022 Francyéllen Teixeira da Silva, Jessica Klöckner Knorst, Lucas Machado Maracci, Vilmar Antônio Ferrazzo, Gabriela Salatino Liedke, Tatiana Bernardon Silva, Mariana Marquezan https://creativecommons.org/licenses/by/4.0 2022-12-19 2022-12-19 22 e237397 e237397 10.20396/bjos.v22i00.8667397 COVID-19 pandemic and impact on dental education https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667471 <p>Aim: The aim of the present study is to assess the perception of undergraduate students on the impact of the COVID-19 pandemic on Brazilian dental education. Methods: This crosssectional study was conducted using an online questionnaire hosted in Google Forms platform and publicized on Instagram<sup>®</sup> and Facebook<sup>®</sup>. The questionnaire was available between July 8-27, 2020. Absolute and relative frequencies were obtained for variables of interest using Pearson’s chi-squared and considering 95% confidence intervals. Prevalence of learning resource variables according to self-reported skin color, educational institution, and Brazilian region were presented using equiplots. Results: A total of 1,050 undergraduate dental students answered the questionnaire. Most students reported being in full-distance learning mode. Among the undergraduate students, 65.4% reported perceiving a very high impact in dental education, and 16.6% of students reported not being able to follow distance learning. In addition, 14% reported not having a personal laptop or desktop to study with, with this condition being more prevalent among non-white than white individuals. Conclusion: We conclude that Brazilian dental students perceived a high impact of COVID-19 on dental education, as well as one-sixth of the students reported not having adequate resources to continue with distance learning. It is important that different policies are developed at the institutional and governmental levels to reduce the impact of the pandemic on dental education.</p> Clarissa de Aguiar Dias Sarah Arangurem Karam Catarina Borges da Fonseca Cumerlato Letícia Regina Morello Sartori Matheus dos Santos Fernandez Nathália Ribeiro Jorge da Silva Camila Hubner Bielavski Alexandre Emidio Ribeiro Silva Eduardo Dickie de Castilhos Marcos Britto Correa Copyright (c) 2022 Clarissa de Aguiar Dias, Sarah Arangurem Karam, Catarina Borges da Fonseca Cumerlato, Letícia Regina Morello Sartori, Matheus dos Santos Fernandez, Nathália Ribeiro Jorge da Silva, Camila Hubner Bielavski, Alexandre Emidio Ribeiro Silva, Eduardo Dickie de Castilhos, Marcos Britto Correa https://creativecommons.org/licenses/by/4.0 2022-12-02 2022-12-02 22 e237471 e237471 10.20396/bjos.v22i00.8667471 Effect of phenylmethylsulfonyl fluoride, a protease inhibitor, on enamel surface remineralization https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8670883 <p>Phenylmethylsulfonyl fluoride (PMSF) is a protease inhibitor widely used in research, but fluoride is released during its action and this knowledge has been neglected in dental research. Aim: to evaluate if fluoride released by salivary protease action on PMSF affects enamel remineralization and fluoride uptake. Methods: Groups of 10 enamel slabs, with caries-like lesions and known surface hardness (SH), were subjected to one of the following treatment groups: Stimulated human saliva (SHS), negative control; SHS containing 1.0 μg F/mL (NaF), positive control; and SHS containing 10, 50 or 100 μM PMSF. The slabs were subjected to a pH-cycling regimen consisting of 22 h/day in each treatment solution and 2 h/day in a demineralizing solution. After 12 days, SH was again measured to calculate the percentage of surface hardness recovery (%SHR), followed by enamel fluoride uptake determination. The time-related fluoride release from 100.0 μM PMSF by SHS action was also determined. Data were analyzed by ANOVA followed by Newman-Keuls test. Results: The release of fluoride from PMSF by SHS was rapid, reaching a maximum value after 10 min. Fluoride released from PMSF was more effective in enhancing %SHR and increasing fluoride uptake in enamel compared with SHS alone (p &lt; 0.05); furthermore, it was equivalent to the positive control (p &gt; 0.05). Conclusion: In conclusion, fluoride released by saliva from PMSF is available to react with enamel and needs to be taken into account in research using this protease inhibitor.</p> Paulo Edelvar Corrêa Peres Jean Fu Domenick T Zero Jaime Aparecido Cury Copyright (c) 2022 Paulo Edelvar Corrêa Peres, Jean Fu, Zero DT, Jaime Aparecido Cury https://creativecommons.org/licenses/by/4.0 2022-11-03 2022-11-03 22 e230883 e230883 10.20396/bjos.v22i00.8670883 Prevalence of depressive symptoms among dental students is influenced by sex, academic performance, smoking exposure, and sexual orientation https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8669237 <p>Aim: To estimate the prevalence and associated factors of self-reported depressive symptoms in undergraduate and graduate dental students. Methods: The Depression, Anxiety and Stress Scale (DASS-21) was applied, and only the depression domain was verified. A structured questionnaire was used to collect sociodemographic, behavioral, and COVID-19 pandemic-related fear variables. Academic performance was assessed based on academic records, ranging from 0 (worst possible grade) to 10 (best possible grade). Respondents included 408 regularly enrolled dental students. Bi- and multivariate analyses were performed using Poisson regression with robust variance to verify the association between at least moderate depressive symptoms and independent variables. Results: The prevalence of at least moderate depression was 40.5% among undergraduate students and 26% among graduate students. The prevalence of fear and anxiety due to the COVID-19 pandemic was 96.1% among undergraduate students and 93.5% among graduate students. In the final multivariate analysis, being female (prevalence ratio [PR]:2.01; 95% confidence interval [95%CI]:1.36–2.96) was associated with a higher PR for depression. Conversely, no exposure to smoking (PR:0.54; 95%CI:0.36–0.82) and a final academic performance average ≥7.0 (PR:0.56; 95%CI:0.41–0.76) was associated with a lower PR for depression. Finally, among graduate students, a non-heterosexual orientation was associated with a higher PR for depression (PR:6.70; 95%CI:2.21–20.29). Conclusion: Higher rates of depression symptoms were observed in female undergraduates, students with lower academic performance and smoking exposure, and graduate dental students with a non-heterosexual orientation.</p> Bruna Oliveira de Freitas Maísa Casarin Rafaela Zazyki de Almeida Jessica Maria Pessoa Gomes Isadora Vilas Boas Cepeda Francisco Wilker Mustafa Gomes Muniz Copyright (c) 2022 Bruna Oliveira de Freitas, Maísa Casarin, Rafaela Zazyki de Almeida, Jessica Maria Pessoa Gomes, Isadora Vilas Boas Cepeda, Francisco Wilker Mustafa Gomes Muniz https://creativecommons.org/licenses/by/4.0 2022-11-03 2022-11-03 22 e239237 e239237 10.20396/bjos.v22i00.8669237 The effectiveness of EDTA 17% as a cleaning solution for the fiber post space after filling with cements https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8666508 <p>Aim: To evaluate the resistance of the union between a glass fiber post and radicular dentine after cleaning the root with 17% EDTA and filling with different endodontic cements. Methods: Forty uniradicular bovine incisors were removed to obtain root lengths of 18 mm. Endodontic treatment was performed on all roots using different filling cements (zinc oxide and eugenol-based, OZE; cement based on epoxy resin, AH) and cleaning solutions (saline, SA or EDTA), which made it possible to obtain four groups: OZE<sub>SA</sub>, OZE<sub>EDTA</sub>, AH<sub>SA</sub> and AH<sub>EDTA</sub>. Subsequently, 12 mm of filling material was removed from the roots, and they were prepared to receive fiber posts luted with resin cement. To execute the mechanical cycles (2x106 cycles, 90 N, 4 Hz), coronal reconstruction was performed with a silicon matrix. The roots were then sliced (2-mm thick) to perform the push-out test. The results were analyzed using analysis of variance (one factor and two factors) and Tukey’s test (α=0,05). Results: Bond strength (Mpa) was significantly higher for OZE<sub>EDTA</sub> (9,18) and AH<sub>EDTA</sub> (8,70) than for OZE<sub>SA</sub> (6,06) AH<sub>SA</sub> (8,7). OZE<sub>EDTA</sub> also presented the highest values in the cervical region (15,18) but was significantly lower in the apical region (2,99). However, AH<sub>EDTA</sub> had a homogeneous bond strength in all thirds. Conclusion: Regardless of the endodontic cement used, EDTA was used as an irrigating solution, culminating in a higher bond strength between the glass fiber post and dentin.</p> Luciana Arruda Mendes de Paula Lohara Campos de Abreu Reis Jean Soares Miranda Francielle Silvestre Verner Rafael Binato Junqueira Rodrigo Furtado de Carvalho Copyright (c) 2022 Luciana Arruda Mendes de Paula, Lohara Campos de Abreu Reis, Jean Soares Miranda, Francielle Silvestre Verner, Rafael Binato Junqueira, Rodrigo Furtado de Carvalho https://creativecommons.org/licenses/by/4.0 2022-11-01 2022-11-01 22 e236508 e236508 10.20396/bjos.v22i00.8666508 Triclosan antimicrobial activity against dental-caries-related bacteria https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8668076 <p>Triclosan (TCS) is a chlorinated diphenyl ether and a possible active agent against microorganisms. Due to its probability of reducing dental plaque accumulation, TCS can be added as a substance for oral hygiene. Aim: To evaluate the efficacy and antimicrobial capacity of TCS against <em>Pseudomonas aeruginosa </em>and <em>Streptococcus mutans</em>. Methods: This work evaluates the percentage of bacteria inhibition of <em>P. aeruginosa </em>(ATCC 27853) and <em>S. mutans </em>(ATCC 25175). TCS concentrations between 2 and 128 μg.mL-1 were tested. Results: An inhibitory potential of TCS was found against <em>S. mutans</em>. No percentage of inhibition was detected against <em>P. aeruginosa </em>(technical and biological triplicate). Conclusion: TCS, an antimicrobial agent used in dentifrices, can reduce <em>S. mutans </em>levels therefore these dentifrices should be indicated for patients with a high risk of caries. However, further study is needed, including antimicrobial analyses against other microbial conditions.</p> Jade Ormondes de Farias Jamilca de Almeida do Espírito Santo Ingrid Aquino Amorim Taia Maria Berto Rezende Copyright (c) 2022 Jade Ormondes de Farias, Jamilca de Almeida do Espírito Santo, Ingrid Aquino Amorim, Taia Maria Berto Rezende https://creativecommons.org/licenses/by/4.0 2022-09-13 2022-09-13 22 e238076 e238076 10.20396/bjos.v22i00.8668076 The peri-implant ligament https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671269 <p>The peri-implant ligament is formed from the interface of bone tissue, through the anchoring of proteins and the surface of the dental implant. In this sense, it is relevant to understand the extent to which this ligament is structured and biomimics the periodontal ligament functions. Aim: The goal of this scoping review is to present and analyze the peri-implant ligament composition and compare the extent to which this ligament is structured and biomimics the periodontal ligament functions. Methods: This scoping review was performed according to the Joanna Briggs Institute methodology for scoping reviews and following the Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for scoping review. Two independent researchers searched Pubmed, Cochrane, Embase, Virtual Health Library, Scielo, Scopus, Web of Science, Brazilian Bibliography of Dentistry, Latin American and Caribbean Literature in Health Sciences, Digital Library of Theses and Dissertations from the University of São Paulo and Portal Capes. Studies published in English, Portuguese and Spanish, over the last 21 years (2000-2021). Results: A total of 330 titles were identified and after applying inclusion and exclusion factors, 27 studies were included in this review. All proteins were identified regarding their tissue function and classified into 6 major protein groups. After that this new protein ligament was compared with the periodontal ligament regarding its function and composition. The main proteins associated with osseointegration, and thus, with the peri-implant ligament are recognized as belonging to the periodontal ligament. Conclusion: This scoping review results suggest evidence of the composition and function of the periimplant ligament. However, variations may still exist due to the existence of several modulants of the osseointegration process.</p> Marvin do Nascimento Bruno Martins de Souza Aline Tany Posch Copyright (c) 2022 Marvin do Nascimento, Bruno Martins de Souza, Aline Tany Posch https://creativecommons.org/licenses/by/4.0 2023-01-26 2023-01-26 22 e231269 e231269 10.20396/bjos.v22i00.8671269