Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study

Authors

  • Janaína Cavalcante Lemos Lemos Universidade Potiguar – UnP
  • Bruno César de Vasconcelos Gurgel Universidade Federal do Rio Grande do Norte – UFRN

DOI:

https://doi.org/10.20396/bjos.v14i2.8640822

Keywords:

Rats, Periodontal diseases, Th1 cells, Th2 cells, Cytokines, Immunohistochemistry

Abstract

Aim: To evaluate the involvement of Th2 cells in different periods of the active phase of experimental periodontal disease and expression of the R1 subunit of the receptor for IFN-γ during the early and advanced progression of the disease. Methods: Experimental periodontitis was induced in 30 male Wistar rats by placing cotton ligatures around the mandibular first molars. The rats were then randomly assigned into two groups: G1=15 and G2=15, in group G1, ligatures were maintained for 2 days, a period that corresponds to the initial stage of periodontal disease in rats, in G2 ligatures were left for 15 days, a period that corresponds to the advanced stage of periodontal disease. The contra-lateral teeth served as controls (unligated). An immunohistochemical investigation of the gingival tissue was performed to detect the presence of the Th2 specific transcription factor (GATA3). Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. IFN-γ R1 was detected at an early stage during the active phase of disease, but the expression of positive cells remained unaltered during the remaining period of the study. Conclusions: These results indicate that the Th2 response may have a protective role during the pathogenesis of experimental periodontal disease, and that the IFN-γ R1 subunit may not be associated with periodontal disease progression.

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Author Biographies

Janaína Cavalcante Lemos Lemos, Universidade Potiguar – UnP

Universidade Potiguar - UnP, Faculdade de Odontologia, Departamento / Área de Periodontia

Bruno César de Vasconcelos Gurgel, Universidade Federal do Rio Grande do Norte – UFRN

Faculdade de Odontologia, Departamento / Área de Periodontia

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Published

2015-05-28

How to Cite

1.
Lemos JCL, Gurgel BC de V. Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study. Braz. J. Oral Sci. [Internet]. 2015 May 28 [cited 2022 Dec. 8];14(2):106-11. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822

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