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Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
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Keywords

Receptors
notch
Gene expression
Stem cells
Tooth
deciduous
Culture media

How to Cite

1.
Taha SAM, Ling JKS, Azmi NIB, Kannan TP, Azlina A, Mokhtar KI. Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium. Braz. J. Oral Sci. [Internet]. 2015 Jul. 1 [cited 2024 Jul. 24];14(2):135-40. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640828

Abstract

Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.
Remote (Português (Brasil))
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This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2015 Siti Aisyah Mohd Taha, Joanne Koh Su Ling, Nur Izyan Binti Azmi, Thirumulu Ponnuraj Kannan, Ahmad Azlina, Khairani Idah Mokhtar

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