Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium

Authors

  • Siti Aisyah Mohd Taha
  • Joanne Koh Su Ling Universiti Sains Malaysia
  • Nur Izyan Binti Azmi Universiti Sains Malaysia
  • Thirumulu Ponnuraj Kannan Universiti Sains Malaysia
  • Ahmad Azlina Universiti Sains Malaysia
  • Khairani Idah Mokhtar Internacional Universiti Malaysia islâmica

DOI:

https://doi.org/10.20396/bjos.v14i2.8640828

Keywords:

Receptors, notch, Gene expression, Stem cells, Tooth, deciduous, Culture media

Abstract

Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.

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Author Biographies

Siti Aisyah Mohd Taha

Universiti Sains Malaysia, Faculdade de Odontologia

Joanne Koh Su Ling, Universiti Sains Malaysia

Universiti Sains Malaysia, Faculdade de Odontologia

Nur Izyan Binti Azmi, Universiti Sains Malaysia

Universiti Sains Malaysia, Faculdade de Odontologia

Thirumulu Ponnuraj Kannan, Universiti Sains Malaysia

Universiti Sains Malaysia, Faculdade de Odontologia e Faculdade de Ciências Médicas, Centro de Genoma Humano

Ahmad Azlina, Universiti Sains Malaysia

Universiti Sains Malaysia, Faculdade de Odontologia

Khairani Idah Mokhtar, Internacional Universiti Malaysia islâmica

Internacional Universiti Malaysia islâmica, Kulliyah de Odontologia

Published

2015-07-01

How to Cite

1.
Taha SAM, Ling JKS, Azmi NIB, Kannan TP, Azlina A, Mokhtar KI. Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium. Braz. J. Oral Sci. [Internet]. 2015 Jul. 1 [cited 2022 Dec. 10];14(2):135-40. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640828

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Article