Agreement between RAPD, API20C AUX, CHROMagar Candida and microculture on oral Candida identification

Authors

  • Emanuene Galdino Pires Universidade Federal da Paraíba - UFPB
  • Edimilson Martins de Freitas Universidade Estadual de Montes Claros - UNIMONTES
  • Paulo Rogério Ferreti Bonan Universidade Federal da Paraíba - UFPB
  • Sérgio Avelino Mota Nobre Universidade Estadual de Montes Claros - UNIMONTES

DOI:

https://doi.org/10.20396/bjos.v14i2.8640830

Keywords:

Candida, Mouth, Methods

Abstract

Aim: To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. After this, agreement among methods using Kappa test was performed. Results: Agreement rates between RAPD and CHROMagar Candida, showed significant accuracy for C. albicans, C. tropicalis, C. dubliniensis and C. krusei (Kappa: 0.760, 0.640, 0.416 and 0.360, respectively, p<0.05). Comparing RAPD results with microculture, the highest agreement was for C. albicans (Kappa: 0.851 - p<0.05) but no significant agreement for C. lusitaniae, C. krusei and C. guilliermondii was obtained (p>0.05). The agreement was significant for all identified species when RAPD (OPE-18) and API 20C AUX (p<0.05) were used. Critical levels of agreement between RAPD and microculture were observed when C. lusitaniae, C. krusei and C. guilliermondii were identified. Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification.

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Author Biographies

Emanuene Galdino Pires, Universidade Federal da Paraíba - UFPB

Universidade Federal da Paraíba - UFPB, Faculdade de Odontologia, Departamento de Odontologia Clínica e Social

Edimilson Martins de Freitas, Universidade Estadual de Montes Claros - UNIMONTES

Universidade Estadual de Montes Claros - UNIMONTES, Faculdade de Odontologia, Departamento de Odontologia Social

Paulo Rogério Ferreti Bonan, Universidade Federal da Paraíba - UFPB

Universidade Federal da Paraíba - UFPB, Faculdade de Odontologia, Departamento de Odontologia Clínica e Social

Sérgio Avelino Mota Nobre, Universidade Estadual de Montes Claros - UNIMONTES

Universidade Estadual de Montes Claros - UNIMONTES, Centro de Ciências Biológicas e da Saúde

Published

2015-07-01

How to Cite

1.
Pires EG, Freitas EM de, Bonan PRF, Nobre SAM. Agreement between RAPD, API20C AUX, CHROMagar Candida and microculture on oral Candida identification. Braz. J. Oral Sci. [Internet]. 2015 Jul. 1 [cited 2022 Nov. 30];14(2):149-53. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640830

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