Chemical salivary composition and its relationship with periodontal disease and dental calculus

Authors

  • Anne Alejandra Hernández-Castañeda Universidad Santo Tomás
  • Gloria Cristina Aranzazu-Moya Universidad Santo Tomás
  • Gerardo Mantilla Mora Universidad Industrial de Santander
  • Dagmar de Paula Queluz Universidade Estadual de Campinas – UNICAMP

DOI:

https://doi.org/10.20396/bjos.v14i2.8640832

Keywords:

Dental calculus, Urea, Periodontal diseases, Saliva

Abstract

Aim: To determine the relationship between the chemical composition of saliva, periodontal disease and dental calculus. Methods: An observational analytical cross-sectional study was conducted with patients over 55 years of age. Ethical principles of autonomy and risk protection were applied according to the international standards. Sociodemographic and diagnosis variables (presence of dental calculus and periodontal status) were considered to measure salivary concentrations of glucose (by the glucose oxidase/peroxidase method, amylase (by the colorimetric test), urea (by the amount of indophenol), total protein (by the Bradford method) and albumin (by the nephelometric method). Patients chewed a sterile rubber band and 3 mL of stimulated saliva were collected. The samples were stored at -5 °C, centrifuged at 2,800 rpm for 10 min, and the supernatant was removed and stored at -20 °C. Data were presented as frequencies and proportions for qualitative variables and measures of central tendency and dispersion for quantitative variables. Data were analyzed by either analysis of variance or Kruskal Wallis test . A p value <0.05 was considered statistically significant. Results: Significant relationships were observed between the concentration of salivary urea and periodontal status (p = 0.03) and the presence of dental calculus and urea (p = 0.04) was demonstrated. Conclusions: A relationship between the salivary urea concentration and the presence of periodontal disease and dental calculus is suggested.

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Author Biographies

Anne Alejandra Hernández-Castañeda, Universidad Santo Tomás

Universidad Santo Tomás, Dentistry School, Department of Oral Medicine, Semiology and Pathology, Colombia

Gloria Cristina Aranzazu-Moya, Universidad Santo Tomás

Universidad Santo Tomás, Dentistry School, Department of Oral Medicine, Semiology and Pathology, Colombia

Gerardo Mantilla Mora, Universidad Industrial de Santander

Universidad Industrial de Santander, School of Medicine, Department of Biochemistry, Colombia

Dagmar de Paula Queluz, Universidade Estadual de Campinas – UNICAMP

Universidade Estadual de Campinas – UNICAMP, Piracicaba Dental School, Department of Community and Preventive Dentistry, Piracicaba

Published

2015-06-30

How to Cite

1.
Hernández-Castañeda AA, Aranzazu-Moya GC, Mora GM, Queluz D de P. Chemical salivary composition and its relationship with periodontal disease and dental calculus. Braz. J. Oral Sci. [Internet]. 2015 Jun. 30 [cited 2022 Dec. 6];14(2):159-65. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640832

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