Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA

Authors

  • Gustavo Giacomelli Nascimento Universidade Federal de Pelotas – UFPel
  • Amauri Antiquera Leite Universidade Estadual Paulista - UNESP
  • Elza Regina Manzolli Leite Universidade Estadual Paulista - UNESP
  • José Eduardo Cezar Sampaio Universidade Estadual Paulista - UNESP
  • Fábio Renato Manzolli Leite Universidade Federal de Pelotas – UFPel

DOI:

https://doi.org/10.20396/bjos.v13i2.8640896

Keywords:

Ethylenediaminetetraacetic acid, Periodontal regeneration, Scanning electron microscopy, Smear layer

Abstract

Aim: To assess the combined use of tetracycline (TTC) and ethylenediaminetetraacetic acid (EDTA) on clot formation, considering that EDTA may neutralize TTC acidity. Methods: Planed human tooth roots were treated with saline solution, EDTA, TTC and their combination (EDTA followed by TTC and TTC before EDTA). Fresh human blood was applied on the conditioned surfaces to check clot adhesion and stabilization. A previously calibrated (kappa = 0.93) and blinded examiner scored scanning electron micrographs of the samples. Statistical analyses were performed using one-way ANOVA and Tukey’s test. Results: Application of TTC before EDTA presented the best results with the highest number of cells adhered to the root surface (p=0.046). Use of EDTA alone and EDTA before TTC disturbed clot stabilization when compared to control group (p<0.01). Conclusions: The use of TTC before EDTA seems to be able to keep blood cells viable to establish an organized clot and could be used by clinicians together with the conventional mechanical root scaling and planing.

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Author Biographies

Gustavo Giacomelli Nascimento, Universidade Federal de Pelotas – UFPel

Universidade Federal de Pelotas – UFPel, School of Dentistry, Department of Semiology and Clinics, Pelotas, RS.

Amauri Antiquera Leite, Universidade Estadual Paulista - UNESP

Universidade Estadual Paulista - UNESP, School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, SP.

Elza Regina Manzolli Leite, Universidade Estadual Paulista - UNESP

Universidade Estadual Paulista - UNESP, School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, SP.

José Eduardo Cezar Sampaio, Universidade Estadual Paulista - UNESP

Universidade Estadual Paulista - UNESP, Araraquara Dental School, Department of Diagnosis and Surgery, Araraquara, SP, Brazil

Fábio Renato Manzolli Leite, Universidade Federal de Pelotas – UFPel

Universidade Federal de Pelotas – UFPel, School of Dentistry, Department of Semiology and Clinics, Pelotas, RS.

Published

2015-10-07

How to Cite

1.
Nascimento GG, Leite AA, Leite ERM, Sampaio JEC, Leite FRM. Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA. Braz. J. Oral Sci. [Internet]. 2015 Oct. 7 [cited 2022 Nov. 30];13(2):83-8. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640896

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