Effects of various hydrogen peroxide bleaching concentrations and number of applications on ename

  • Sandrine Bittencourt Berger Universidade do Norte do Paraná - UNOPAR
  • Luis Eduardo Silva Soares Universidade do Vale do Paraíba - Univap
  • Airton Abrahão Martin Universidade do Vale do Paraíba - Univap
  • Gláucia Maria Bovi Ambrosano Universidade Estadual de Campinas - UNICAMP
  • Cínthia Pereira Machado Tabchoury Universidade Estadual de Campinas - UNICAMP
  • Marcelo Giannini Universidade Estadual de Campinas - UNICAMP

Abstract

Aim: To evaluate the effects of three hydrogen peroxide (HP) concentrations on enamel mineral content (MC), following three peroxide applications and using three methods to measure the MC. Methods: Forty samples were obtained from bovine incisors and randomly divided into 4 groups (n=10). The control group remained untreated, while the experimental groups were tested for three HP concentrations (10%, 35% and 50%). The HP gel was applied 3 times on the enamel surface for 30 min per application and the samples were analyzed after each application. The MC of the enamel was determined before and after bleaching using Fourier transform (FT-Raman) spectroscopy and micro energy-dispersive x-ray fluorescence spectrometry (µEDXRF). The calcium (Ca) lost from the bleached enamel was quantified with an atomic absorption spectrometer (AAS). The data were analyzed statistically by ANOVA, Tukey and Dunnett´s tests (p<0.05). Results: The FT-Raman showed a decrease in MC for all bleaching treatments, without influence of the different HP concentrations or the number of applications. µEDXRF did not detect any changes in MC. Conclusions: Ca loss was observed by the AAS, with no difference among the three HP concentrations. The FT-Raman and AAS analyses detected MC reduction and Ca loss after HP bleaching

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Author Biographies

Sandrine Bittencourt Berger, Universidade do Norte do Paraná - UNOPAR
Universidade do Norte do Paraná - UNOPAR, School of Dentistry, Department of Restorative Dentistry, Londrina, PR.
Luis Eduardo Silva Soares, Universidade do Vale do Paraíba - Univap
Universidade do Vale do Paraíba - Univap, Research and Development Institute (IP&D), Laboratory of Biomedical Vibrational Spectroscopy, São José dos Campos, SP.
Airton Abrahão Martin, Universidade do Vale do Paraíba - Univap
Universidade do Vale do Paraíba - Univap, Research and Development Institute (IP&D), Laboratory of Biomedical Vibrational Spectroscopy, São José dos Campos, SP.
Gláucia Maria Bovi Ambrosano, Universidade Estadual de Campinas - UNICAMP
Universidade Estadual de Campinas - UNICAMP, Piracicaba Dental School, Department of Community Dentistry and Biostatistics, Piracicaba, SP.
Cínthia Pereira Machado Tabchoury, Universidade Estadual de Campinas - UNICAMP
Universidade Estadual de Campinas - UNICAMP, Piracicaba Dental School, Department of Restorative Dentistry, Piracicaba, SP.
Marcelo Giannini, Universidade Estadual de Campinas - UNICAMP
Universidade Estadual de Campinas - UNICAMP, Piracicaba Dental School, Department of Restorative Dentistry, Piracicaba, SP.
Published
2015-10-13
How to Cite
Berger, S. B., Soares, L. E. S., Martin, A. A., Ambrosano, G. M. B., Tabchoury, C. P. M., & Giannini, M. (2015). Effects of various hydrogen peroxide bleaching concentrations and number of applications on ename. Brazilian Journal of Oral Sciences, 13(1), 22-27. https://doi.org/10.20396/bjos.v13i1.8640925
Section
Article

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