In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

Authors

  • Bruna Paloma de Oliveira Universidade Federal de Pernambuco - UFPE
  • Carla Cabral dos Santos Accioly Lins Universidade Federal de Pernambuco - UFPE
  • Fátima Alves Diniz Universidade Federal de Pernambuco - UFPE
  • Liliane Lima Melo Universidade Federal de Pernambuco - UFPE
  • Célia Maria Machado Barbosa de Castro Universidade Federal de Pernambuco - UFPE

DOI:

https://doi.org/10.20396/bjos.v13i1.8640933

Keywords:

Endodontics, Enterococcus faecalis, Methylene blue, Microbiology, Photodynamic therapy

Abstract

Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results:The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Bruna Paloma de Oliveira, Universidade Federal de Pernambuco - UFPE

Universidade Federal de Pernambuco - UFPE, School of Dentistry, Department of Prosthodontics and Oral Facial Surgery, Recife, PE.

Carla Cabral dos Santos Accioly Lins, Universidade Federal de Pernambuco - UFPE

Universidade Federal de Pernambuco - UFPE, Department of Anatomy, Recife, PE.

Fátima Alves Diniz, Universidade Federal de Pernambuco - UFPE

Universidade Federal de Pernambuco - UFPE, Laboratory of Immunopathology Keiso Asami - LIKA, Recife, PE.

Liliane Lima Melo, Universidade Federal de Pernambuco - UFPE

Universidade Federal de Pernambuco - UFPE, Laboratory of Immunopathology Keiso Asami - LIKA, Recife, PE.

Célia Maria Machado Barbosa de Castro, Universidade Federal de Pernambuco - UFPE

Universidade Federal de Pernambuco - UFPE, Department of Tropical Medicine, Recife, PE.

Published

2015-10-13

How to Cite

1.
Oliveira BP de, Lins CC dos SA, Diniz FA, Melo LL, Castro CMMB de. In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms. Braz. J. Oral Sci. [Internet]. 2015 Oct. 13 [cited 2022 Sep. 26];13(1):53-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640933

Issue

Section

Article

Most read articles by the same author(s)