In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

  • Bruna Paloma de Oliveira Universidade Federal de Pernambuco - UFPE
  • Carla Cabral dos Santos Accioly Lins Universidade Federal de Pernambuco - UFPE
  • Fátima Alves Diniz Universidade Federal de Pernambuco - UFPE
  • Liliane Lima Melo Universidade Federal de Pernambuco - UFPE
  • Célia Maria Machado Barbosa de Castro Universidade Federal de Pernambuco - UFPE

Abstract

Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results:The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.

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Author Biographies

Bruna Paloma de Oliveira, Universidade Federal de Pernambuco - UFPE
Universidade Federal de Pernambuco - UFPE, School of Dentistry, Department of Prosthodontics and Oral Facial Surgery, Recife, PE.
Carla Cabral dos Santos Accioly Lins, Universidade Federal de Pernambuco - UFPE
Universidade Federal de Pernambuco - UFPE, Department of Anatomy, Recife, PE.
Fátima Alves Diniz, Universidade Federal de Pernambuco - UFPE
Universidade Federal de Pernambuco - UFPE, Laboratory of Immunopathology Keiso Asami - LIKA, Recife, PE.
Liliane Lima Melo, Universidade Federal de Pernambuco - UFPE
Universidade Federal de Pernambuco - UFPE, Laboratory of Immunopathology Keiso Asami - LIKA, Recife, PE.
Célia Maria Machado Barbosa de Castro, Universidade Federal de Pernambuco - UFPE
Universidade Federal de Pernambuco - UFPE, Department of Tropical Medicine, Recife, PE.
Published
2015-10-13
How to Cite
Oliveira, B. P. de, Lins, C. C. dos S. A., Diniz, F. A., Melo, L. L., & Castro, C. M. M. B. de. (2015). In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms. Brazilian Journal of Oral Sciences, 13(1), 53-57. https://doi.org/10.20396/bjos.v13i1.8640933
Section
Article