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In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms
Remote (Português (Brasil))


Enterococcus faecalis
Methylene blue
Photodynamic therapy

How to Cite

Oliveira BP de, Lins CC dos SA, Diniz FA, Melo LL, Castro CMMB de. In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms. Braz. J. Oral Sci. [Internet]. 2015 Oct. 13 [cited 2023 Jun. 7];13(1):53-7. Available from:


Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results:The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.
Remote (Português (Brasil))

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