A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth

Authors

  • Emmanuel João Nogueira Leal Silva UNICAMP
  • Carolina Bluzarca Rollemberg
  • Tauby de Souza Coutinho Filho State University of Rio de Janeiro (UERJ)
  • Renato Liess Krebs State University of Rio de Janeiro (UERJ)
  • Alexandre Augusto Zaia UNICAMP

DOI:

https://doi.org/10.20396/bjos.v12i2.8641064

Keywords:

Tooth avulsion, Fibroblasts, Cell viability

Abstract

Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2 , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). Results from each test were compared by one-way analysis of variance (ANOVA). Results: Statistical analysis showed that whole milk and HBSS were the most effective media in maintaining cell viability at all tested times (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects. Conclusions: This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests.

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Author Biographies

Emmanuel João Nogueira Leal Silva, UNICAMP

Department of Restorative Dentistry, Area of Endodontic, Piracicaba Dental School, University of Campinas, Piracicaba, SP.

Carolina Bluzarca Rollemberg

Dentist, Rio de Janeiro, RJ.

Tauby de Souza Coutinho Filho, State University of Rio de Janeiro (UERJ)

Department of Endodontic, State University of Rio de Janeiro (UERJ), Rio de Janeiro, RJ.

Renato Liess Krebs, State University of Rio de Janeiro (UERJ)

Department of Endodontic, State University of Rio de Janeiro (UERJ), Rio de Janeiro, RJ.

Alexandre Augusto Zaia, UNICAMP

Department of Restorative Dentistry, Area of Endodontic, Piracicaba Dental School, University of Campinas, Piracicaba, SP.

Published

2015-10-16

How to Cite

1.
Silva EJNL, Rollemberg CB, Coutinho Filho T de S, Krebs RL, Zaia AA. A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth. Braz. J. Oral Sci. [Internet]. 2015 Oct. 16 [cited 2022 Dec. 9];12(2):90-4. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064

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