Proliferation of human periodontal ligament mesenchymal cells on polished and plasma nitriding titanium surfaces

Authors

  • Rodrigo Alves Ribeiro Federal University of Rio Grande do Norte
  • Rodrigo Gadelha Vasconcelos Federal University of Rio Grande do Norte
  • Fernanda Ginani Federal University of Rio Grande do Norte
  • José Sandro Pereira da Silva Federal University of Rio Grande do Norte
  • Clodomiro Alves Júnior Federal University of Rio Grande do Norte
  • Carlos Augusto Galvão Barboza Federal University of Rio Grande do Norte

DOI:

https://doi.org/10.20396/bjos.v12i2.8641102

Keywords:

Biocompatible materials, Titanium, Cell proliferation, Periodontal ligament

Abstract

Aim: To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. Methods: Eighteen titanium disks were polished and half of them (n=9) were submitted to plasma nitriding using the cathodic cage technique. Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. Cell proliferation was analyzed and growth curves were constructed for the different groups by determining the number of cells adhered to the different surfaces at 24, 48 and 72 h after plating. Results: Higher cell number was observed for the nitrided surface at 24 and 48 h. However, no statistically significant difference in cell proliferation was observed between the two different surface treatments (p>0.05). Conclusions: We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. Associated to other physical and chemical properties, it is possible to assume the feasibility of plasma nitriding method and its positive effect on the early cellular events of osseointegration.

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Author Biographies

Rodrigo Alves Ribeiro, Federal University of Rio Grande do Norte

Area of Oral and Maxillofacial Surgery, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN.

Rodrigo Gadelha Vasconcelos, Federal University of Rio Grande do Norte

Area of Oral and Maxillofacial Surgery, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN.

Fernanda Ginani, Federal University of Rio Grande do Norte

Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN.

José Sandro Pereira da Silva, Federal University of Rio Grande do Norte

Area of Oral and Maxillofacial Surgery, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN

Clodomiro Alves Júnior, Federal University of Rio Grande do Norte

Department of Mechanical Engeneering, Federal University of Rio Grande do Norte, Natal, RN.

Carlos Augusto Galvão Barboza, Federal University of Rio Grande do Norte

Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN.

Published

2015-10-16

How to Cite

1.
Ribeiro RA, Vasconcelos RG, Ginani F, Silva JSP da, Alves Júnior C, Barboza CAG. Proliferation of human periodontal ligament mesenchymal cells on polished and plasma nitriding titanium surfaces. Braz. J. Oral Sci. [Internet]. 2015 Oct. 16 [cited 2022 Dec. 10];12(2):143-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641102

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