Banner Portal
Determination of enamel and coronal dentin microhardness


Microhardness. Enamel. Coronal dentin

How to Cite

Cirano FR, Romito GA, Todescan JH. Determination of enamel and coronal dentin microhardness. Braz. J. Oral Sci. [Internet]. 2015 Nov. 12 [cited 2024 May 24];2(6):258-63. Available from:


The Vickers microhardness of enamel and coronal dentin of 30 canines was evaluated. The incisal portion of the crown was discarded by means of a transverse section made 5mm, coronally, from the cemento-enamel junction located on the buccal surface obtaining, thus, the coronal specimen, on which measurements on 3 regions of each dental tissue was carried out. The mean value of microhardness at the E1 region (0.05 to 0.1 mm from the external surface) was of 395.92 VHN and at the E2 region (0.3 to 0.5 mm from the external surface) was of 339.41 VHN, E1 and E2 being, statistically different. The mean microhardness at the E3 region (0.05 to 0.1 mm from the dentinoenamel junction), for the free surfaces, was of 255.02 VHN, and for the proximal surfaces, was of 269.72 VHN. Following statistical analysis (Variance Analysis Test, p < 0.05), we concluded that enamel microhardness decreased from the external surface towards the dentinoenamel junction. The mean microhardness of coronal dentin was obtained in 3 regions: the DC1 region (0.05 to 0.1 mm from the dentinoenamel junction) - with a mean of 38.68 VHN; the DC2 region (0.3 to 0.5 mm from the dentinoenamel junction) - with a mean of 56.42 VHN; and the DC3 region (0.8 to 1.0 mm from the dentinoenamel junction) - with a mean of 36.49 VHN. Microhardness at DC2 was statistically greater than that of DC1, which, in turn was greater than that at DC3.


Büyükyilmaz T, Ogaard B, Rolla G. The resistance of titanium tetrafluoride-treated human enamel to strong hydrochloric acid. Eur J Oral Sci 1997; 105: 473-7.

Bartlett DW, Smith BGN, Wilson RF. Comparison of the effect of fluoride and non-fluoride toothpaste on tooth wear in vitro and the influence of enamel fluoride concentration and hardness of enamel. Br Dent J 1994; 176: 346-8.

Featherstone JDB, ten Cate JM, Shariati M, Arends J. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 1983; 17: 385-91.

Phillips RW, Swartz ML. Effect of fluorides on hardness of tooth enamel. J Am Dent Assoc 1948; 37: 1-13.

Saunsbury PH, Atkinson HF. An investigation into the hardness of human enamel. Part II – Studies of deciduous and permanent teeth. Br Dent J 1953; 94: 251-3.

Fukuda K, Higashi S, Miake K, Matsui T. Studies on microhardness of the human third molar, especially that of the enamel. Bull Tokyo Dent Coll 1975; 16: 11-33.

Meredith N, Sherriff M, Setchell DJ, Swanson SA. Measurement of the microhardness and Young’s modulus of human enamel and dentine using an indentation technique. Arch Oral Biol 1996; 41: 539-45.

Caldwell RC, Muntz ML, Gilmore RW, Pigman W. Microhardness studies of intact surface enamel. J Dent Res 1957; 36: 732-8.

Craig RG, Peyton FA. The microhardness of enamel and dentin. J Dent Res 1958; 37: 661-8.

Craig RG, Gehring PE, Peyton FA. Relation of structure to the microhardness of human dentin. J Dent Res 1959; 38:624- 30.

Rautiola CA, Craig RG. the microhardness of cementum and underlying dentin of normal teeth and teeth exposed to periodontol disease. J Periodontol 1961; 32: 113-23.

Shannon IL, Keuper JB. Microhardness of human dentin: baseline values and effects of fluorides. J Miss Dent Assoc 1976; 56: 11-8.

Banerjee A, Sherriff M, Kidd EA, Watson TF. A confocal microscopic study relating the autofluorescence of carious dentine to its microhardness. Br Dent J 1999; 187: 206-10.

Ryge G, Foley DE, Fairhurst CW. Micro-identation hardness. J Dent Res 1961; 40: 1116-26.

Mjör IA. Human coronal dentine: structure and reactions. Oral Surg Oral Med Oral Pathol 1972; 33: 810-23.

Gaspersic D. Enamel microhardness and histological features of composite enamel pearls of different size. J Oral Pathol Med 1995; 24: 153-8.

Newbrun E, Pigman W. The hardness of enamel and dentine. Aust Dent J 1960; 5: 210-7.

Gustafson G, Gustafson AG. Humam dental enamel in polarized light and contact-microradiography. Acta Odont Scand 1961; 19: 259-87.

Kinney JH, Balooch M, Marshall SJ, Marshall Jr. GW, Weihs TR. Hardness and Young’s modulus of human peritubular and intertubular dentine. Arch Oral Biol 1996; 41: 9-13.

Marshall GW. Dentin microstructure and characterization. Quintessence Int 1993; 24: 606-17.

Fosse G, Saele PK, Eibe R. Numerical density and distribuitional pattern of dentine tubules. Acta Odontol Scand 1992; 50: 201-10.

Pashley D, Okabe A, Parham P. The relationship between dentin microhardness and tubule density. Endod Dent Traumatol 1985; 1:176-9.

The Brazilian Journal of Oral Sciences uses the Creative Commons license (CC), thus preserving the integrity of the articles in an open access environment.


Download data is not yet available.