Effect of vitamin A administration in gamma-irradiated repair tissue as revealed by polarizing microscopy

Authors

  • Patricia Maria Usberti Vizioli Piracicaba Dental School/University of Campinas
  • Mário Roberto Vizioli Piracicaba Dental School/University of Campinas
  • Frab Norberto Boscolo Piracicaba Dental School/University of Campinas
  • Solange Maria Almeida Piracicaba Dental School/University of Campinas
  • Rosana Aramaki T Tanaka Piracicaba Dental School/University of Campinas

DOI:

https://doi.org/10.20396/bjos.v2i6.8641706

Keywords:

Vitamin A. Gamma radiation. Repair tissue. Polarizing microscopy

Abstract

This work was performed in order to evaluate the effect of systemic administration of vitamin A against the damages caused by gamma radiation in rat repair tissue. It were used 48 animals. An incision was made in the dorsal region of the animals, which were separated into four experimental groups, composed of 12 animals each, as follows: Control group (CG) corresponding to the animals which were only submitted to the surgical procedure; Irradiated group (IG), composed by rats that have received 6 Gy of total body gamma irradiation 72 hours after the surgery; Vitamin A group (VAG), where the rats were treated with 15,000 UI of aqueous solution of vitamin A i.p. 48 hours after the wound infliction and Vitamin A irradiated group (VAIG) formed by animals treated in the same way as VAG but gamma-irradiated as IG. After the histological procedures, the repair tissues were observed at 4, 7, 14 and 21 days of development by means of qualitative and quantitative birefringence. The results pointed out to a very effective protection of the repair tissue performed by vitamin A against gamma irradiation.

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Author Biographies

Patricia Maria Usberti Vizioli, Piracicaba Dental School/University of Campinas

Possui graduação em Odontologia pelo União das Faculdades Herminio Ometto(1999), ensino-fundamental-primeiro-graupela Sud Mennucci(1991), ensino-medio-segundo-graupela Sud Mennucci(1994) e aperfeicoamento em Endodontia pela Universidade Estadual de Campinas(2000). Atuando principalmente nos seguintes temas:Radiação ionizante, radioprotetor, vitamina A, reparação tecidual, cicatrização. 

Mário Roberto Vizioli, Piracicaba Dental School/University of Campinas

Possui graduação em Odontologia pela Universidade Estadual de Campinas(1960) e doutorado em Biologia e Patologia Buco-Dental [Piracicaba] pela Universidade Estadual de Campinas(1968).

Frab Norberto Boscolo, Piracicaba Dental School/University of Campinas

Possui graduação em Odontologia pela Universidade Estadual de Campinas (1967) e doutorado em Radiologia Odontológica pela Universidade Estadual de Campinas (1978). 

Solange Maria Almeida, Piracicaba Dental School/University of Campinas

Possui graduação em Odontologia pela Universidade Federal do Ceará (1986), mestrado em Radiologia Odontológica pela Universidade Estadual de Campinas (1992) e doutorado em Radiologia Odontológica pela Universidade Estadual de Campinas (1997). 

Rosana Aramaki T Tanaka, Piracicaba Dental School/University of Campinas

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho- UNESP-ATA (1996) e mestrado em Radiologia Odontológica pela Universidade Estadual de Campinas FOP-UNICAMP (2002).

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Published

2015-11-12

How to Cite

1.
Vizioli PMU, Vizioli MR, Boscolo FN, Almeida SM, Tanaka RAT. Effect of vitamin A administration in gamma-irradiated repair tissue as revealed by polarizing microscopy. Braz. J. Oral Sci. [Internet]. 2015 Nov. 12 [cited 2022 Dec. 5];2(6):272-3. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641706

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