Polymorphisms in the IL-1a and IL-1b genes are not associated with susceptibility to chronic periodontitis in a brazilian population

Authors

  • Paula C. Trevilatto Department of Odontology, Pontifical Catholic University of Paraná
  • Raquel M. Scarel-Caminaga Division of Oral Biology, University of Sagrado Coração
  • Rui B. Brito Junior Department of Morphology, Faculty of Odontology of Piracicaba – UNICAMP
  • Ana P. Souza Division of Oral Biology, University of Sagrado Coração
  • Antonio W. Sallum Department of Morphology, Faculty of Odontology of Piracicaba – UNICAMP
  • Sergio R. P. Line Department of Periodontology, Faculty of Odontology of Piracicaba – UNICAMP

DOI:

https://doi.org/10.20396/bjos.v2i7.8641716

Keywords:

IL-1. Polymorphism. Periodontitis

Abstract

The aim of this study was to investigate the association between polymorphisms in the IL-1a and IL-1b genes and severity of chronic periodontitis in Brazilians. 113 non-smoking subjects over 25 years (mean age 41.6) were divided according to the severity level of periodontal disease: 44 healthy individuals (control group), 31 subjects with moderate and 38 with severe periodontitis. DNA was obtained from epithelial cells through a mouthwash with 3 % glucose and scraping of oral mucosa. The samples were analyzed for IL-1a (- 889) and IL-1b (+3953) polymorphisms using PCR-RFLP. The significance of the differences in the frequencies of the polymorphisms in the control and groups with periodontitis was assessed by Chisquare test (p<0.05). No significant differences in the allele and genotype frequencies were found among the groups. It was concluded that neither polymorphisms IL-1a (-889) and IL-1b (+3953) nor the composite genotype were associated with the severity of chronic periodontal disease in the study population.

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Author Biographies

Paula C. Trevilatto, Department of Odontology, Pontifical Catholic University of Paraná

bachelor's at Odontology from Universidade Estadual de Campinas (1996), bachelor's at Bacharelado Em Ciências Biológicas from Universidade Estadual Paulista Júlio de Mesquita Filho (1990), master's at Odontology from Universidade Estadual de Campinas (1999) and doctorate at Biologia Buco-Dental from Universidade Estadual de Campinas (2002). Has experience in Odontology, acting on the following subjects: periodontite, polimorfismos, polimorfismo, polymorphism and mmp-1.

Raquel M. Scarel-Caminaga, Division of Oral Biology, University of Sagrado Coração

Graduated in Biological Sciences from the State University of Campinas (1997), Master in Pathology Oral-Dental Biology from the State University of Campinas (2000), PhD in Oral-Dental Biology from the State University of Campinas (2002) and Postdoctoral Fellow in Mutagenesis the Faculty of Medicine of Ribeirão Preto, FMRP-USP (2010). Currently, I am working as Adjunct Professor in the School of Dentistry at Araraquara - UNESP, Univ Estadual Paulista, responsible for teaching Human Genetics. Have experience in dentistry, with emphasis on Genetics Susceptibility to Periodontal Disease with or without Diabetes and Dyslipidemia, particularly investigating: genetic polymorphisms and their functionality, gene expression, Immunogenetics, dysmorphology and Down Syndrome. As a new line of research I have been investigating cytotoxicity, genotoxicity and mutagenicity of Biomaterials.

Rui B. Brito Junior, Department of Morphology, Faculty of Odontology of Piracicaba – UNICAMP

bachelor's at Odontologia from Universidade Estadual de Campinas (1997), master's at Odontology from Universidade Estadual de Campinas (2000) and doctorate at Odontology from Universidade Estadual de Campinas (2003). Has experience in Odontology, acting on the following subjects: doença periodontal, pcr-rflp, biologia molecular, genética and polimorfismo.

Antonio W. Sallum, Department of Morphology, Faculty of Odontology of Piracicaba – UNICAMP

Possui graduação em Odontologia pela Universidade Estadual de Campinas(1967), especialização em Periodontia pela Universidade Estadual de Campinas(1980), especialização em Endodontia pela Universidade Estadual de Campinas(1980) e doutorado em Ciências Periodontia pela Universidade Estadual de Campinas(1973). Atualmente é professor titular da Universidade Estadual de Campinas.

Sergio R. P. Line, Department of Periodontology, Faculty of Odontology of Piracicaba – UNICAMP

Graduado em Odontologia pela Universidade Estadual de Campinas (1985). Atualmente é Professor Titular da Faculdade de Odontologia de Piracicaba da Universidade Estadual de Campinas (FOP/Unicamp).

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Published

2015-11-12

How to Cite

1.
Trevilatto PC, Scarel-Caminaga RM, Brito Junior RB, Souza AP, Sallum AW, Line SRP. Polymorphisms in the IL-1a and IL-1b genes are not associated with susceptibility to chronic periodontitis in a brazilian population. Braz. J. Oral Sci. [Internet]. 2015Nov.12 [cited 2021Oct.27];2(7):348-52. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641716

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