Microbiological evaluation of adjunctive systemic and local tetracycline administration combined with scaling and root planing in the treatment of chronic periodontitis

Authors

  • Ana Paula Colombo Dept. of Medical Microbiology - Federal University of Rio de Janeiro
  • Cristiane Gonçalves Dept. of Dental Clinic – Periodontics – Gama Filho University
  • Rosa Maria J. Rodrigues Dept. of Dental Clinic – Periodontics – Federal University of Rio de Janeiro
  • Renata Souto Dept. of Medical Microbiology - Federal University of Rio de Janeiro
  • Milton Uzeda Dept. of Medical Microbiology - Federal University of Rio de Janeiro
  • Eduardo Jorge Feres-Filho Dept. of Dental Clinic – Periodontics – Gama Filho University

DOI:

https://doi.org/10.20396/bjos.v2i7.8641722

Keywords:

periodontitis/therapy. Tetracycline/therapeutic use. Local drug deliver. DNA probes. Checkerboard. Subgingival microbiota

Abstract

The purpose of this study was to evaluate the microbiological changes in the subgingival biofilm resulting from systemic or local tetracycline therapy associated with scaling and root planing (SRP) in chronic periodontitis. Thirty patients with chronic periodontitis were randomly assigned to 3 groups: SRP + 500 mg of systemic tetracycline twice/day for 14 days; SRP alone and SRP + tetracycline fibers at 4 selected sites for 10 days. Subgingival plaque samples were obtained from 4 selected sites with probing pocket depths of 6 - 10 mm in each subject at baseline, 1 week, 3 and 6 months post-therapy. The presence and levels of 31 subgingival species distributed into microbial complexes were determined using the checkerboard technique. Significant microbiological changes within and among groups were sought using the Friedman and Kruskal-Wallis tests. Most of the microbial complexes decreased in prevalence and levels at 1 week and 3 months, but tended to return to baseline levels at 6 months after treatment. Beneficial species were reduced over time in subjects who received SRP only, however were increased or maintained in the patients treated with local and systemic tetracycline. Moreover, these groups showed a significant reduction (p < 0.01) in the prevalence of the “red complex” species as compared to the control. Likewise, A. actinomycetemcomitans was more decresed in both tetracycline groups. In chronic periodontitis, local and systemic tetracycline therapy significantly reduced the prevalence of some periodontal pathogens and favored the increase of beneficial species for at least 6 months after treatment.

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Author Biographies

Ana Paula Colombo, Dept. of Medical Microbiology - Federal University of Rio de Janeiro

Graduated in Dentistry (DMD) from Universidade Federal do Rio de Janeiro (1990). Obtained a certificate in Periodontology from Universidade federal do Rio de Janeiro (1991) and Doctor In Medical Sciences In Oral Biology (DMSc) from Harvard University (1997) and a certificate in Oral Microbiology at The Forsyth Institute, under the supervision of Dr. Sig Socransky. In 2007-2008, took a sabbatical at The Forsyth Institute, under the supervision of Dr. Bruce Paster. Has experience in Odontology, acting on the following subjects: checkerboard, periodontite, periodontitis, subgingival microbiota and dna probes. The major research areas are Periodontology, Oral Microbiology and Immunology, Biofilm control, DNA probes, checkerboard, DNA microarrays.

Renata Souto, Dept. of Medical Microbiology - Federal University of Rio de Janeiro

bachelor's at Bacharelado em Microbiologia e Imunologia from Instituto de Microbiologia Prof. Paulo de Góes (2000), master's at Microbiology from Universidade do Estado do Rio de Janeiro (2003) and doctorate at Ciências from Instituto de Microbiologia Prof. Paulo de Góes (2007). Has experience in Microbiology, focusing on Microbiology, acting on the following subjects: checkerboard dna-dna hybridization, periodontite, tetraciclina, sondas de dna and hiv

Milton Uzeda, Dept. of Medical Microbiology - Federal University of Rio de Janeiro

bachelor's at Odontologia from Universidade Federal do Rio de Janeiro (1969), master's at Microbiology from Universidade Federal do Rio de Janeiro (1973) and doctorate at Microbiology from Universidade Federal do Rio de Janeiro (1982). Has experience in Microbiology, focusing on Bacteriology, acting on the following subjects: microbiota oral, bacterias anaerobias, bactérias anaeróbias, infecções endodônticas and doenca periodontal.

Eduardo Jorge Feres-Filho, Dept. of Dental Clinic – Periodontics – Gama Filho University

Possui graduação em Odontologia pela Universidade Federal do Rio de Janeiro (1978), mestrado em Odontologia (Periodontia) pela Universidade Federal do Rio de Janeiro (1991), doutorado em Oral Biology - Boston University (1995), residencia/pos-doutorado na University of Texas - Houston Dental Branch (1996) e pos-doutorado na Boston University - Oral Biology (2007).

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Published

2015-11-12

How to Cite

1.
Colombo AP, Gonçalves C, Rodrigues RMJ, Souto R, Uzeda M, Feres-Filho EJ. Microbiological evaluation of adjunctive systemic and local tetracycline administration combined with scaling and root planing in the treatment of chronic periodontitis. Braz. J. Oral Sci. [Internet]. 2015 Nov. 12 [cited 2022 Nov. 29];2(7):370-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641722

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