Healing and cytotoxic effects of Psidium guajava (Myrtaceae) leaf extracts

Authors

  • Kristianne Porta Santos Fernandes Nove de Julho University
  • Sandra Kalil Bussadori Nove de Julho University
  • Márcia Martins Marques University of São Paulo
  • Nilsa Sumie Yamashita Wadt Nove de Julho University
  • Erna Bach Nove de Julho University
  • Manoela Domingues Martins Federal University of Rio Grande do Sul

DOI:

https://doi.org/10.20396/bjos.v9i4.8641730

Keywords:

Psidium guajava, Wound healing, Cell culture, Guava leaves, Cytotoxicity

Abstract

Aim: The aims of this study were to evaluate the wound healing potential in vivo and the cytotoxic effects in vitro of Psidium guajava (Myrtaceae) leaf extract and commonly used corticosteroids. Methods: The healing effect was studied in vivo by the clinical and histological evaluation of traumatic lesions in the oral mucosa of rats treated with these substances. Each rat received 2 daily applications of the medicine tested and the animals were sacrificed after 1, 3, 5, 7 and 14 days. Tissue sections stained with hematoxylin & eosin were analyzed. The histological evaluation involved a 5-point scoring system based on the degree of healing, ranging from 1 (total repair of epithelium and connective tissue) to 5 (epithelial ulcer and acute inflammatory infiltrate). The Kruskal-Wallis test was used for statistical analysis of the histological scores. For the in vitro toxicity assay, each substance was applied to mucosa fibroblast cell cultures in conditioned media. The media were conditioned by placing the substances in contact with fresh culture medium for 24 h. The cytotoxicity analysis was performed using the MTT assay. Data were analyzed statistically by ANOVA and Tukey’s test at 5% significance level. Results: In vitro, the guava extract caused a decrease in the cell viability and growth when compared to the control and corticosteroids. In vivo, guava extract caused accelerated wound healing from the 3rd day, whereas the corticosteroids delayed tissue repair and were associated with bacterial surface colonization, the presence of micro-abscesses and intense inflammatory infiltrate in the submucosa. Conclusions: Although in a short-term cytotoxicity analysis, the guava extract reduced the cell population in vitro, while in vivo, the extract accelerated wound healing.

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Author Biographies

Kristianne Porta Santos Fernandes, Nove de Julho University

PhD, Full Professor, Department of Rehabilitation Sciences, Nove de Julho University, São Paulo, SP

Sandra Kalil Bussadori, Nove de Julho University

PhD, Full Professor, Department of Rehabilitation Sciences, Nove de Julho University, São Paulo, SP

Márcia Martins Marques, University of São Paulo

PhD, Full Professor, Department of Dentistry, Dental School, University of São Paulo, São Paulo, SP

Nilsa Sumie Yamashita Wadt, Nove de Julho University

PhD, Full Professor, School of Pharmacy, Nove de Julho University, São Paulo, SP

Erna Bach, Nove de Julho University

PhD, Full Professor, School of Pharmacy, Nove de Julho University, São Paulo, SP

Manoela Domingues Martins, Federal University of Rio Grande do Sul

PhD, Full Professor, Department of Oral Pathology, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, RS

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Published

2015-11-16

How to Cite

1.
Fernandes KPS, Bussadori SK, Marques MM, Wadt NSY, Bach E, Martins MD. Healing and cytotoxic effects of Psidium guajava (Myrtaceae) leaf extracts. Braz. J. Oral Sci. [Internet]. 2015Nov.16 [cited 2021Jun.20];9(4):449-54. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641730

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