Banner Portal
Conventional X-ray densitometry detects osteopenia in ovariectomized young rats. Short communication


Osteoporosis. Bone densitometry. Animal models. Rat

How to Cite

Zecchin KG, Pereira MC, Vargas PA, Graner E, Jorge Júnior J. Conventional X-ray densitometry detects osteopenia in ovariectomized young rats. Short communication. Braz. J. Oral Sci. [Internet]. 2015 Nov. 13 [cited 2023 Jun. 1];3(8):425-7. Available from:


The present study investigated the reduction on bone density 4 weeks after ovariectomy in rats, with conventional X-ray densitometry. Eighty female Wistar rats underwent bilateral ovariectomy (OVX group) or sham operation (sham group) under general anesthesia. Animals were killed by cervical dislocation 4 weeks after surgery. The left tibia of each animal was dissected and radiographed using oclusal films. Radiographs were scanned and virtual squares on the proximal tibial metaphysis were analyzed with proper software. Higher OD values represent darker areas in the X-ray. After that the tibia were decalcified with EDTA and serial transversal sections with 6 µm of the mesial root of the first mandibular molar were stained with hematoxylin-eosin. Digital images were captured and the densitometric volume of bone was evaluated using software. A significant increase of dark areas in the radiographies of OVX animals was observed when compared with control group (control=1.136±0.020 vs OVX=1.269±0.027, t test, p=0.01). Histomorphometric analysis showed a significant reduction on bone density of OVX animals (control=125.8±20.5 vs OVX=65.4±0.0154, t test, p=0.01). Conventional X-ray densitometry is useful for the characterization of osteopenia in rats after ovariectomy. Besides, 4 weeks are sufficient to cause significant decrease on bone content after ovariectomy.


Weber K, Goldberg M, Stangassinger M, Erben RG. 1alphahydroxyvitamin D2 is less toxic but not bone selective relative to 1alpha-hydroxyvitamin D3 in ovariectomized rats. J Bone Miner Res 2001; 16: 639-51.

Kinney JH, Haupt DL, Balooch M, Ladd AJ, Ryaby JT, Lane NE. Three-dimensional morphometry of the L6 vertebra in the ovariectomized rat model of osteoporosis: biomechanical implications. J Bone Miner Res 2000; 15: 1981-91.

Sunyer T, Lewis J, Collin-Osdoby P, Osdoby P. Estrogen’s bone-protective effects may involve differential IL-1 receptor regulation in human osteoclast-like cells. J Clin Invest 1999; 103: 1409-18.

Kim HJ, Bae YC, Park RW, Choi SW, Cho SH, Choi YS et al. Bone-protecting effect of safflower seeds in ovariectomized rats. Calcif Tissue Int 2002; 71: 88-94.

Roudebush RE, Magee DE, Benslay DN, Bendele AM, Bryant HU. Effect of weight manipulation on bone loss due to ovariectomy and the protective effects of estrogen in the rat. Calcif Tissue Int 1993; 53: 61-4.

Goulding A, Gold E, Lewis-Barned NJ. Effects of hysterectomy on bone in intact rats, ovariectomized rats, and ovariectomized rats treated with estrogen. J Bone Miner Res 1996; 11: 977- 83.

Marcondes FK, Bianchi FJ, Tanno AP. Determination of the estrous cycle phases of rats: some helpful considerations. Braz J Biol 2002; 62: 609-14.

Bauss F, Wagner M, Hothorn LH. Total administered dose of ibandronate determines its effects on bone mass and architecture in ovariectomized aged rats. J Rheumatol 2002; 29: 990-8.

The Brazilian Journal of Oral Sciences uses the Creative Commons license (CC), thus preserving the integrity of the articles in an open access environment.


Download data is not yet available.


Metrics Loading ...