Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts

  • Rogério Lacerda dos Santos Federal University of Alfenas; Federal University of Rio de Janeiro
  • Matheus Melo Pithon Federal University of Alfenas; Federal University of Rio de Janeiro
  • Fernanda Otaviano Martins Federal University of Rio de Janeiro-UFRJ
  • Maria Teresa Villela Romanos Federal University of Rio de Janeiro
Keywords: Cytotoxicity, Elastics, Biocompatibility, Orthodontics

Abstract

Aim: To test the hypothesis that there is no difference in the cytotoxicity among natural latex elastics of different manufacturers using a L929 cell line culture. Methods: Different latex intraoral elastics (I.D. = 5/16", 4.5 oz.) were tested. The sample was divided into 7 groups of 15 elastics each: Group AO (American Orthodontics), Group GAC (GAC International), Group TP (TP Orthodontics), Group AD (Aditek), Group AB (Abzil), Group MO (Morelli) and Group UN (Uniden). Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). The cytotoxicity was evaluated by using the “dye-uptake” test, which was employed at two different moments (1 and 24 h). Data were compared by ANOVA and Tukey’s test (P < 0.05). Results: The results showed a significant difference (P < 0.05) between all groups and the group CC (cell control) at 1 and 24 h. Groups AD, AB, MO and UN were noticeably more cytotoxic than the groups AO, GAC and TP at 1 h. After 24 h, a significant decrease in cell viability was observed in all groups. Conclusions: Intraoral elastics from American Orthodontics, GAC and TP Orthodontics trademarks induced less cell lysis than Aditek, Abzil, Morelli and Uniden trademarks

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Author Biographies

Rogério Lacerda dos Santos, Federal University of Alfenas; Federal University of Rio de Janeiro
Specialist in Orthodontics, Federal University of Alfenas, Minas Gerais; Master in Orthodontics, Federal University of Rio de Janeiro; PhD student in Orthodontics, Federal University of Rio de Janeiro.
Matheus Melo Pithon, Federal University of Alfenas; Federal University of Rio de Janeiro
Specialist in Orthodontics, Federal University of Alfenas, Minas Gerais; Master in Orthodontics, Federal University of Rio de Janeiro; PhD student in Orthodontics, Federal University of Rio de Janeiro.
Fernanda Otaviano Martins, Federal University of Rio de Janeiro-UFRJ
Graduated in Biological Sciences: Microbiology and Immunology Federal University of Rio de Janeiro-UFRJ
Maria Teresa Villela Romanos, Federal University of Rio de Janeiro
Assistant Professor of Microbiology, Federal University of Rio de Janeiro
Published
2015-11-18
How to Cite
Santos, R. L. dos, Pithon, M. M., Martins, F. O., & Romanos, M. T. V. (2015). Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts. Brazilian Journal of Oral Sciences, 9(3), 366-370. https://doi.org/10.20396/bjos.v9i3.8641794
Section
Article

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