Effects of ozonated water on Candida albicans oral isolates

Authors

  • Ivan da Silva Faria Taubate University
  • Mariko Ueno Taubate University
  • Cristiane Yumi Koga-Ito São José dos Campos School of Dentistry
  • Wilfredo Irrazabal Urruchi Instituto Tecnológico da Aeronáutica
  • Ivan Balducci São José dos Campos School of Dentistry
  • Antonio Olavo Cardoso Jorge Taubate University

DOI:

https://doi.org/10.20396/bjos.v4i14.8641835

Keywords:

Ozone. Candida albicans. Mouth

Abstract

Forty-nine C. albicans freshly isolated strains and C. albicans ATCC 18804 were included in the study. Initially, suspensions containing 5 x 108 viable cells/ml were prepared. This suspension was submitted to a concentration of 3.3 mg/ml of ozone for 20s, 40s, 60s, 80s, 100s 120s, 140s, 160s, 180s and 300s. Before the experiment and also after each period of exposition, 0.1 ml of dilutions of the final suspension was plated on Sabouraud dextrose agar and incubated at 37°C for 24-48 h. After this period, the number of colony forming units was calculated. Data were statistically analyzed by Pearson’s correlation test. A reduction of the microorganism’s concentration during the contact time with ozone was observed. Complete inactivation was observed only for standard strain after 5 min. Freshly isolated C. albicans strains showed higher resistance to ozone. A linear correlation between the logarithm of yeast concentration and period of contact was found in both cases, being the linear correlation coefficients significant in the experiments

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Author Biographies

Ivan da Silva Faria, Taubate University

bachelor's at Ciências Biológicas from Universidade de Taubaté (1985) and master's at Odontology from Universidade Estadual Paulista Júlio de Mesquita Filho (2002). Has experience in Microbiology, focusing on Medical Microbiology, acting on the following subjects: iga anti-candida, candida, candida albicans, prótese total and staphylococcus aureus.

Mariko Ueno, Taubate University

has graduation at Ciências Biológicas - Modalidade Médica by Universidade Estadual Paulista Júlio de Mesquita Filho (1994) , graduation at Ciências Biológicas - Licenciatura by Universidade Estadual Paulista Júlio de Mesquita Filho (1996) , master's at Saúde Pública by Universidade de São Paulo (2000) and Ph.D. at Saúde Pública by Universidade de São Paulo (2005) . Currently is Revisor de periódico of Revista de Saúde Pública / Journal of Public Health, Revisor de periódico of Neotropical Entomology, exercício liminar of Escola de Artes, Ciências e Humanidades - USP, of Revista de Saúde Pública - USP, of Universidade de São Paulo e Revisor de periódico of Revista Colombiana de Entomología. Has experience in the area of Parasitology , with emphasis on Entomologia Médica. Focused, mainly, in the subjects: Anopheles cruzii, Anopheles bellator, estratificação vertical, bromélia-malária, CDC+CO2 e bromélias.

Cristiane Yumi Koga-Ito, São José dos Campos School of Dentistry

Possui graduação em Odontologia pela UNESP, S. Jose dos Campos (1993), mestrado (1995) e doutorado em Biologia Patologia Buco-Dental pela UNICAMP (1997). Realizou pós-doutorado na Universidade de Turim- Itália (1997-1999) na área de Micologia Médica e na Universidade Federal de Minas Gerais na área de Micologia e Biologia Molecular (2000-2002). Realizou estágio pós-doutoral no Instituto Carlos Malbran (Buenos Aires, 2003) em antifúngicos. Esteve na Universidade de Hong Kong (2010) como pesquisador visitante, trabalhando em biofilmes e microscopia confocal. Atualmente é Professora Adjunta da UNESP, S. Jose dos Campos, atuando na graduação e pós-graduação. 

Wilfredo Irrazabal Urruchi, Instituto Tecnológico da Aeronáutica

graduate at Física from Universidad Nacional Mayor de San Marcos (1987), master's at Physic from Instituto Tecnológico de Aeronáutica (1992) and ph.d. at Physic from Instituto Tecnológico de Aeronáutica (1998). Has experience in Physic, focusing on Plasma Physics and Electrical Discharges, acting on the following subjects: ozônio, plasma, aplicação de ozônio, tratamento de água and plasma jet.

Ivan Balducci, São José dos Campos School of Dentistry

Graduate at Engenharia Metalúrgica from Escola de Engenharia Mauá (1977) and master's at Mechanical Engineering from Universidade Estadual Paulista Júlio de Mesquita Filho (2002). Has experience in Probability and Statistics, acting on the following subjects: assessoria estatística, microinfiltração, canais radiculares, ligas de alumínio and infiltração marginal.

Antonio Olavo Cardoso Jorge, Taubate University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1977), Especialização em Biologia e em Endodontia, Mestrado em Biologia (Fisiopatologia Celular) pela Universidade de Taubaté (1986), mestrado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1988), Doutorado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1991) e Livre-Docência em Microbiologia e Imunologia pela Faculdade de Odontologia de São José dos Campos/UNESP (1995). Atua como Professor Titular de Microbiologia e Imunologia da Universidade Estadual Paulista Júlio de Mesquita Filho (Faculdade de Odontologia de São José dos Campos/UNESP), desde 2002.

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Published

2015-11-19

How to Cite

1.
Faria I da S, Ueno M, Koga-Ito CY, Urruchi WI, Balducci I, Jorge AOC. Effects of ozonated water on Candida albicans oral isolates. Braz. J. Oral Sci. [Internet]. 2015 Nov. 19 [cited 2022 Nov. 26];4(14):783-6. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641835

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