Forty-nine C. albicans freshly isolated strains and C. albicans ATCC 18804 were included in the study. Initially, suspensions containing 5 x 108 viable cells/ml were prepared. This suspension was submitted to a concentration of 3.3 mg/ml of ozone for 20s, 40s, 60s, 80s, 100s 120s, 140s, 160s, 180s and 300s. Before the experiment and also after each period of exposition, 0.1 ml of dilutions of the final suspension was plated on Sabouraud dextrose agar and incubated at 37°C for 24-48 h. After this period, the number of colony forming units was calculated. Data were statistically analyzed by Pearson’s correlation test. A reduction of the microorganism’s concentration during the contact time with ozone was observed. Complete inactivation was observed only for standard strain after 5 min. Freshly isolated C. albicans strains showed higher resistance to ozone. A linear correlation between the logarithm of yeast concentration and period of contact was found in both cases, being the linear correlation coefficients significant in the experiments
Restaino L, Frampton EW, Hemphill JB, Palnikar P. Efficacy of ozonated water against various food-related microorganisms. App Environ Microbiol 1995; 61: 3471-5.
Lezcano I, Rey RP, Gutiérrez MS, Baluja C, Sánchez E Ozone inactivation of microorganisms in water. Gram-positive bacteria and yeast. Ozone Sci Eng 2001; 23: 183-7.
Shin GA, Sobsey MD. Reduction of norwalk virus, poliovirus 1, and bacteriophage by ozone disinfection of water. Appl Environ Microbiol 2003; 69: 3975-8.
Lee JY, Deininger RA. Survival of bacteria after ozonation. Ozone Sci Eng 2000; 22: 65-75.
Rodrigues LP, Cepero MS, Perdomo EO. Effectos del ozono em el tratamiento de la gingivoestomatitis herpética aguda. Rev Cubana Estomatol 1994; 31: 14-7.
Guerra OC. Aplicación de la ozonoterapia em el tratamiento de la alveolitis. Rev Cubana Estomatol 1997; 34: 21-4.
Baysan A, Whiley RA, Lych E. Antimicrobial effect of a novel ozone-generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res 2000; 34: 498-501.
Velano HE, Nascimento LC, Barros LM, Panzeri J. In vitro evaluation of the antibacterial activity of ozonized water against Staphylococcus aureus. Pesq Odontol Bras 2001; 15: 18-22.
Murakami H, Sakuna S, Nakamura K, Ito Y, Hattori M, Asai A et al. Disinfection of removable dentures using ozone. Dent Mater J 1996; 15: 220-5.
Oizumi M, Suzuki T, Uchida M, Furya J, Okamoto Y. In vitro testing of a denture cleaning method using ozone. J Med Dent Sci 1998; 45: 135-9.
Baley JE. Neonatal candidiasis: the current challenge. Clin Perinatol 1991;18: 263 -79.
Saballs P, Torres-Rodrigues JM, Salvadó M La candidemia em el síndrome de inmunodeficiencia adquirida. Estudio retrospectivo de nueve casos. Rev Iberoam Micol 2000; 17: 2-5.
Sandvén P. Laboratory identification and sensitivity testing of yeast isolates. Acta Odontol Scand 1990; 48: 27-36.
Williams DW, Lewis MAO. Isolation and identification of Candida from the oral cavity. Oral Dis 2000; 6: 3-11.
Gurley B. Ozone; pharmaceutical sterilant of the future? J Parent Sci Tech 1985; 39: 256-61.
Yamayoshi T, Tatsumi N. Microbicidal effects of ozone solution on methicillin-resistant Staphylococcus aureus. Drugs Exp Clin Res 1993; 19: 59-64.
Türk A. Ozone in dental medicine. Ozonnachrichten 1985; 4: 61-5.
Filippi A. Water disinfection of dental units using ozone – microbiological results after 11 years and technical problems. Ozone Sci Eng 2002; 24: 479-83.
Williams HN, Kelley J, Folineo D, Williams GC, Hawley CL, Sibinski J. Assessing microbial contamination in clean water dental units and compliance with disinfection protocol. J Am Dent Assoc 1994; 125: 1205-11.
Odds FC. Resistance of yeasts to azole-derivative antifungals. J Antimicrob Chemoth 1999; 39: 1696-9.
Saballs P, Torres-Rodrígues JM, Salvadó M. La candidemia en el síndrome de inmunodeficiencia adquirida. Estudo retrospectivo de nueve casos. Rev Iberoam Micol 2000; 17: 2-5.
Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004; 30: 778-81.
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