Antimicrobial activity of chlorhexidine in root canals instrumented with the ProTaper Universal™ System

  • Andréa Cruz Câmara Federal University of Pernambuco
  • Miracy Muniz de Albuquerque Federal University of Pernambuco
  • Carlos Menezes Aguiar Federal University of Pernambuco
  • Ana Cristina Regis de Barros Correia Federal University of Pernambuco
Keywords: Antimicrobial activity, Chlorhexidine, ProTaper Universal™, Root canal irrigant, Rotary instrumentation

Abstract

Aim: The purpose of the present study was to evaluate the antimicrobial activity of 0.2%, 1%, and 2% chlorhexidine in root canals instrumented with the ProTaper Universal™ system. Methods: Fifty human mandibular premolar teeth were infected with a mixture of Candida albicans, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus. The specimens were randomly divided into 5 groups with 10 root canals according to the irrigant used. All root canals were instrumented with the ProTaper Universal™ system. Assessment of the antimicrobial action of the irrigant was performed before, during, and after instrumentation. Data were analyzed statistically by Chi-squared test and the Fisher exact test at 5% significance level. Results: The 0.2% chlorhexidine solution was ineffective against all test microorganisms. The 1% chlorhexidine solution was effective in eliminating P. aeruginosa and C. albicans after the use of the F1 and F3 instruments, respectively. The 2% chlorhexidine solution was effective at killing S. aureus, P. aeruginosa and C. albicans after the use of the S1 instrument. There were statistically significant differences (p<0.05) between the concentrations of chlorhexidine and the instruments used. Conclusions: The 0.2% chlorhexidine solution in combination with rotary instrumentation was ineffective against all test microorganisms. The 1% chlorhexidine solution was ineffective against S. aureus and E. faecalis. The 2% chlorhexidine solution was not sufficient to inactivate E. faecalis.

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Author Biographies

Andréa Cruz Câmara, Federal University of Pernambuco
PhD Student in Pharmaceutical Sciences, Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Federal University of Pernambuco
Miracy Muniz de Albuquerque, Federal University of Pernambuco
AssociateProfessor, Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Federal University of Pernambuco
Carlos Menezes Aguiar, Federal University of Pernambuco
AssociateProfessor, Department of Prosthodontics and Oral and Maxillofacial Surgery, Dental School, Federal University of Pernambuco
Ana Cristina Regis de Barros Correia, Federal University of Pernambuco
MSc Student in Fungal Biology, Department of Pharmaceutical Sciences, Federal University of Pernambuco
Published
2015-11-18
How to Cite
Câmara, A. C., Albuquerque, M. M. de, Aguiar, C. M., & Correia, A. C. R. de B. (2015). Antimicrobial activity of chlorhexidine in root canals instrumented with the ProTaper Universal™ System. Brazilian Journal of Oral Sciences, 9(3), 402-409. https://doi.org/10.20396/bjos.v9i3.8641838
Section
Article