Systemic evaluation of various haemostatic agents following local application prior to direct pulp capping

Authors

  • Franklin Garcia-Godoy Nova Southeastern University, College of Dental Medicine
  • Peter E. Murray Nova Southeastern University, College of Dental Medicine

DOI:

https://doi.org/10.20396/bjos.v4i14.8641842

Keywords:

Sodium hypochlorite. Epinephrine. Heart rate. Blood pressure. Healing

Abstract

Limited physiological information is available regarding the role of hemostatic agents to control the success of direct pulp capping treatment in order to help save vital pulps from pulpotomy, pulpectomy or extraction. The aim of this study was to test whether placement of various hemostatic agents would alter short-term pulp healing following direct pulp capping. Class V cavity preparations with pulp exposures were prepared in 31-nonhuman primate teeth, pulp hemorrhage was controlled by placement of epinephrine, Consepsis®, sodium hypochlorite or combinations of these agents. Each exposure was then direct capped with composite resin and then observed immediately or after 13, and 28-days. Histological examination of pulp healing was conducted according to ISO standards for pulp inflammation, soft tissue organization, reactionary and reparative dentin bridge formation. Physiological measurement of heart rate and blood pressure were continuously recorded during the three operative procedures. Local pulp treatment with the various hemostatic agents did not alter systemic blood pressure or heart rate during local pulp application. All hemostatic treatments provided acceptable biocompatibility. Minor differences in pulp healing were observed, but there were no statistically significant differences between treatments (Pe”0.05). We conclude that hemostatic treatment had little effect on systemic pulp physiology or healing.

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References

Hafez, AA, Kopel HM, Cox CF. Pulpotomy reconsidered: An application of an adhesive system to pulpotomized permanent primate pulps. Quintessence Int 2000; 21: 579-89.

Mjor IA. Pulp-dentin biology in restorative dentistry. Part 7: The exposed pulp. Quintessence Int 2002; 33: 113-35.

Murray PE, Smith AJ. Saving pulps–a biological basis. Prim Dent Care 2002;9:21-26 4. Dummer PM, Hicks R, Huws D. Clinical signs and symptoms in pulp disease. Int Endod J 1980; 13: 27-35.

Matsuo T, Nakanishi T, Shimizu H, Ebisu S. A clinical study of direct pulp capping applied to carious-exposed pulps. J Endod 1996; 22: 551-6.

Hunter FA. Saving pulps. A Queer Process, Items of Interest 1883; 5: 352-3.

Seelig A. The formation of calcified tissue in dental pulps. N Y St Dent J 1956; 22: 260-72.

Hebling J, Giro E, Costa C. Biocompatibility of an adhesive system applied to exposed human dental pulp. J Endod 1999; 25: 676-82.

Horsted P, El Attar K, Langeland K. Capping of monkey pulps with Dycal and a Ca-eugenol cement. Oral Surg Oral Med Oral Pathol 1981; 52: 531-53.

Kitasako Y, Inokishi S, Fujitani M, Otsuki M, Tagami J. Shortterm reaction of exposed monkey pulp beneath adhesive resins. Oper Dent 1998; 23: 308-17.

Schröder U, Granath LE. Early reaction of inert human teeth to Ca(OH)2 following pulpotomy and its significance to the development of the hard tissue barrier. Odontol Revy 1971; 22: 379-95.

Schröder U. Effects of Ca(OH)2 containing pulp-capping agents on pulp cell migration, proliferation, and differentiation. J Dent Res 1985; 64: 541-8.

Fei AL, Udin RD, Johnson R. A clinical study of ferric sulfate as a pulpotomy agent in primary teeth. Ped Dent 1991; 13: 327-32.

Oztas N, Ulusu T, Oygur T, Cokpekin F. Comparison of elecrtosurgery and formocresol as pulpotomy techniques in dog primary teeth. J Clin Ped Dent 1994; 22: 447-9.

Wilkerson MK, Hill SD, Arcoria CJ. Effects of the argon laser on primary tooth pulpotomies in swine. J Clin Laser Med Surg 1996; 14: 37-42.

Dakin HD. On the use of certain antiseptic substances in the treatment of infected wound. Br Med J 1915; 2: 318-20.

Walker A. A definite and dependable therapy for pulpless teeth. J Am Dent Assoc 1936; 23: 1418.

Masterton JB. Chemical debridement in the treatment of infected pulpless teeth and chronic periapical abscess. Dent Pract 1965; 15: 162-70.

Hirota K. A study on partial pulp removal (pulpotomy) using four different tissue solvents. J Jpn Stomatol Soc 1959; 26: 1588-1603.

Sudo C. A study on partial pulp removal (pulpotomy) using NaOCl (sodium hypochlorite). J Japanese Stomatological Soc 1959; 26: 1012-24.

Cox CF, Hafez AA, Akimoto N, Otsuki M, Suzuki S, Tarim B. Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primates. Am J Dent 1998;10: 55-63.

Cox CF, Hafez AA, Akimoto N, Otsuki M, Mills JC. Biological basis for clinical success: Pulp protection and the toothrestoration interface. Pract Periodontics Aesthet Dent 1999; 11: 819-26.

Senia ES, Marshall FJ, Rosen S. The solvent action of sodium hypochlorite on pulp tissue of extracted teeth. Oral Surg Oral Med Oral Pathol 1971; 31: 96-103.

Rosenfeld EF, James GA, Burch BS. Vital pulp tissue response to sodium hypochlorite. J Endod 1978; 4: 140-6.

Teixeira LS. Demarco FF. Coppola MC. Bonow ML. Clinical and radiographic evaluation of pulpotomies performed under intrapulpal injection of anaesthetic solution. Int Endod J 2001; 34: 440-6.

Thomas GP. Boyd JB. Soni NN. Palmer JE. Histologic study of pulp capping using chlorhexidine in dogs. NDA J 1995; 46: 17-20.

Cox CF. Evaluation and treatment of bacterial microleakage. Am J Dent 1994; 7: 293-5.

Cox CF, Subay RK, Ostro E, Suzuki S. Tunnel defects in dentin bridges: Their formation following direct pulp capping. Oper Dent 1996; 21 :4-11.

Cox CF, Keall CL, Keall HJ, Ostro E, Bergenholtz G. Biocompatibility of surface-sealed dental materials against exposed pulps. J Prosthet Dent 1987; 57: 1-8.

Akimoto N, Momoi Y, Kohno A, Suzuki S, Otsuki M, Suzuki S, Cox CF. Biocompatibility of Clearfil Liner Bond 2 and Clearfil AP-X system on nonexposed and exposed primate teeth. Quintessence Int 1998; 29: 177-88.

ISO 7405 Preclinical evaluation of biocompatibility of medical devices used in dentistry; test methods for dental materials. Geneva, Switzerland: International Organization for Standardization; 1997.

McKay GS. Gram stain modified to improve colour contrast. J Clin Pathol 1970;23:191.

Dawson-Saunders B, Trapp RG. Basic and clinical biostatistics. 2nd ed. Norwalk, CT; Appleton and Lange; 1994.

Kitasako Y, Inokishi S, Tagami J. Effect of direct resin pulp capping techniques on short-term response of mechanically exposed pulps. J Dent 1999; 27: 257-63.

Cox CF. Hafez AA. Biocomposition and reaction of pulp tissues to restorative treatments. Dent Clinics N Am 2001; 45: 31-48.

Murray PE, Hafez AA, Smith AJ, Cox CF. Hierarchy of pulp capping and repair activities responsible for dentin bridge formation. Am J Dent 2002; 15: 236-43

Yoshiba K, Yoshiba N, Iwaku M. Histological observations of hard tissue barrier formation in amputated dental pulp capped with tricalcium phosphate containing calcium hydroxide. Endod Dent Traumatol 1994; 10: 113-20.

Pameijer CH, Stanley HR. The disastrous effects of the “Total Etch” technique in vital pulp capping in primates. Am J Dent 1998;11(Spec Iss) S45-S59.

Schuurs AH. Gruythuysen RJ. Wesselink PR. Pulp capping with adhesive resin-based composite Vs calcium hydroxide: a review. Endod Dent Traumatol 2000; 16: 240-50.

Pereira JC. Segala AD. Costa CA. Human pulpal response to direct pulp capping with an adhesive system. Am J Dent 2000; 13: 139-47.

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Published

2015-11-19

How to Cite

1.
Garcia-Godoy F, Murray PE. Systemic evaluation of various haemostatic agents following local application prior to direct pulp capping. Braz. J. Oral Sci. [Internet]. 2015 Nov. 19 [cited 2022 Nov. 28];4(14):791-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641842