A comparative study of p53 expression in hyperplastic , dysplastic epithelium and oral squamous cell carcinoma

Authors

  • Sucheta Bansal College of Dental Sciences and Research
  • Keya Sircar College of Dental Sciences and Research
  • Sanjeev Kumar Joshi College of Dental Sciences and Research
  • Sanjeet Singh College of Dental Sciences and Research
  • Varun Rastogi College of Dental Sciences and Research

DOI:

https://doi.org/10.20396/bjos.v9i2.8641851

Keywords:

Dysplasia P53, Oral squamous cell carcinoma, Oral leukoplakia

Abstract

Aim: To study oral hyperplastic epithelium, dysplastic epithelium and squamous cell carcinoma to determine (1) the prevalence of p53 protein immunoreactivity, (2) number of p53 positive cells, and (3) the area of localization of p53 protein immunoreactivity. Methods: Two contiguous sections from 30 tissue specimens (10 each from oral hyperplastic epithelium, dysplastic epithelium and squamous cell carcinoma) were subjected to hematoxylin and eosin (H/E) staining for histopathological diagnosis and immunohistochemical (IHC) staining for demonstration of p53. p53 positivity was looked for in each IHC stained slide and the number of positive cells amongst 1,000 epithelial cells were recorded. The localization of these p53 positive cells within the strata (i.e. basal/suprabasal, spinous and superficial layers) of epithelium between 3 groups, and also within each group according to histological grades was recorded. Results: Higher p53 positive cell counts were demonstrated in oral squamous cell carcinoma compared to hyperplastic and dysplastic tissues. The expression of p53 in epithelial hyperkeratosis was mainly localized to basal epithelial cells whereas in epithelial dysplasia, it was predominantly localized to spinous epithelial cells. Conclusions: Qualitatively p53 is not a specific marker for malignancy of oral epithelium. However the quantitative analysis of p53 positive cells and their localization in oral epithelium is of importance as a marker for oral squamous cell carcinoma.

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Author Biographies

Sucheta Bansal, College of Dental Sciences and Research

MDS, Post-Graduate, Department of Oral and Maxillofacial Pathology, DJ College of Dental Sciences and Research

Keya Sircar, College of Dental Sciences and Research

MDS, Professor, Department of Oral and Maxillofacial Pathology, DJ College of Dental Sciences and Research

Sanjeev Kumar Joshi, College of Dental Sciences and Research

MDS, Department of Prosthodontics Including Crown and Bridge and Implantology, DJ College of Dental Sciences and Research

Sanjeet Singh, College of Dental Sciences and Research

MDS, Department of Oral and Maxillofacial Pathology, DJ College of Dental Sciences and Research

Varun Rastogi, College of Dental Sciences and Research

MDS, Department of Oral and Maxillofacial Pathology, DJ College of Dental Sciences and Research

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Published

2015-11-25

How to Cite

1.
Bansal S, Sircar K, Joshi SK, Singh S, Rastogi V. A comparative study of p53 expression in hyperplastic , dysplastic epithelium and oral squamous cell carcinoma. Braz. J. Oral Sci. [Internet]. 2015 Nov. 25 [cited 2022 Dec. 1];9(2):85-8. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641851

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