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Abstract
P53 gene is commonly found to be mutated in human cancer. Currently, limited data exist on the occurrence of p53 gene mutation in ameloblastomas. This study designed to evaluate the expression of p53 proteins and their gene mutations in human ameloblastomas. Four cases of epithelial odontogenic tumors were used. Normal cells of a 7 months aborted fetus’s mandible were used as negative control. P53 Protein expression was detected immunohistochemically. DNA was extracted and amplified using PCR. Gene sequences analyzed to determine p53 mutation. p53 was overexpressed in one case of unicystic ameloblastoma and 2 cases of variant types of ameloblastoma. The Coding sequencing analyses demonstrated p53 gene alteration in cases of ameloblastoms in Exon 7 while, fetus epithelial mucosal cells showed the wild type DNA sequence in Exon 4. In conclusion p53 protein overexpression was detected by immunohistochemical staining as well as by DNA sequence analysis in cases of ameloblastomas.References
Thompson I O, van Rensburg L J, Phillips VMJ. Desmoplastic ameloblastoma: correlative histopathology, radiology and CTMR imaging. J Oral Pathol Med 1996; 25(7):405–10.
Waldron C A, Odontogenic tumors. In: Oral and maxillofacial pathology. Neville BW, Damm DD, Allen CM, Bouquot JE, editors. Philadelphia: W.B. Saunders Company.2002; p. 611.
Sirichitra V, Dhiravarangkura P. Intrabony ameloblastoma of the jaws. An anaylsis of 147 Thai patients. Int J Oral Surg 1984; 13:187-93.
Tan W T L, The L Y, Ho K H, Lee ST. Mandibular ameloblastoma in Singapore- A10 year review. Ann. Acad Med Singapore. 1986; 15: 378-83.
Kameyama Y, Takehana S, Mizohata M, et al. Aclonicopathological study of ameloblastomas.Int J Oral Maxillofac Surg.1987; 16:706-12.
Ng KH, Siar C H, Chuah C H. Ameloblastoma in Malaysian children. Asian J Oral Maxillofac Surg.1989; 1:57-62.
Ng KH, Siar C H. Peripheral ameloblastoma with clear cell differentiation. Oral Surg Oral Med Oral Pathol.1990; 70:210- 3.
Hoffman S, Jacoway J R, Krolls SO. Intraosseous and parosteal tumors of the jaws. In: Hartmann WH, Sobin LH, editors. Atlas of tumor pathology, vol. 24. Washington (DC): Armed Forces Institute of Pathology.1987; p. 90–125.
Becelli R, Carbon A, Cerulli G, Perugini M and Iannetti G. Mandibular ameloblastoma: analysis of surgical treatment carried out in 60 patients between 1977 and 1998. J Craniofacial Surg. 2002; 13 pp. 395–400.
Ord R A, Blanchaert R.H.J., Nikitakis N.G. and Sauk J.J., Ameloblastoma in children. J Oral Maxillofac Surg 2002; 60, 762–770.
Ciment L.M. and Ciment A.J., Malignant ameloblastoma metastatic to the lungs 29 years after primary resection: a case report. Chest 2002; 121, pp. 1359–1361.
Zwahlen R.A. and Gratz K.W., Maxillary ameloblastomas: a review of the literature and of a 15-year database. J Craniomaxillofac Surg 2002; 30, pp. 273–279.
Neville BW, Damm DD, Allen CM, Bouquot JE. Odontogenic cysts and tumors. In: Waldron CA, editor. Oral and maxillofacial pathology. Philadelphia (PA): W.B. Saunders Company.1995; pp. 493–538.
Shatkin S, Hoffmeister FS. Ameloblastoma: a rational approach to therapy. Oral Surg 1965; 20: 421-435.
Moe H, Clausen F, Philipsen HP. The ultrastructure of the simple ameloblastoma. Acta Path Microbiol Scand 1961; 52: 140-154.
El-Sissy N A. Eastern Mediterranean Health journal.1999; 5: 478-489.
Ueno S, Mushimoto K, Shirasu R. Prognostic evaluation of ameloblastoma based on histologic and radiographic typing. J. Oral Maxillofac. Surg. 1989; 47: 11-15.
Gardner D.G., An orderly approach to the study of odontogenic tumors in animals. J Comp Pathol. 1992; 107, pp. 427–438.
Hoffman S, Jacoway JR, Krolls SO. Intraosseous and parosteal tumors of the jaws. In: Hartmann WH, Sobin LH, editors. Atlas of tumor pathology, vol. 24. Washington (DC): Armed Forces Institute of Pathology 1987; p. 90–125.
Becelli R, Carboni A, Cerulli G, Perugini M and Iannetti G. Mandibular ameloblastoma: analysis of surgical treatment carried out in 60 patients between 1977 and 1998. J Craniofac Surg. 2002; 13, pp. 395–400.
Ciment L M and Ciment A J. Malignant ameloblastoma metastatic to the lungs 29 years after primary resection: a case report. Chest. 2002; 121, pp. 1359–1361. (Abstract-MEDLINE) 22. Ord R A, Blanchaert R H J, Nikitakis N G, and Sauk J J. Ameloblastoma in children. J Oral Maxillofac Surg. 2002; 60, pp. 762–770.
Zwahlen R A. and Gratz K W. Maxillary ameloblastomas: a review of the literature and of a 15-year database. J Craniomaxillofac Surg. 2002; 30, pp. 273–279.
Snead M L, Luo W, Hsu D D, Melrose R J, Lau E C. and Stenman G. Human ameloblastoma tumors express the amelogenin gene. Oral Surg Oral Med Oral Pathol .1992; 74, pp. 64–72.
Komisar A. Plexiform ameloblastoma of the maxilla with extension to the skull base. Head & Neck Surgery 1984; 7; 172- 175.
Siar C H, Ng K.H. Ameloblastoma in Malaysia-25 years review. Annals Academy of Medicine Singapore. 1993;22(6): 856-60
Yaacob H. The Radiographic Appearance of Ameloblastoma in Malaysians. Singapore Medical Journal 1991; 32: 70-72
Ramanathan K., Peace Indrani Chelvanayagam, Ng Kok Han, Jayanthi Ramanathan. Ameloblastomas – A Clinicopathologic Study of 133 Cases in Peninsular Malaysia. Medical Journal Malaysia March 1982; 37(1): 18-24
Moe H, Clausen F, Philipsen HP. The ultrastructure of the simple ameloblastoma. Acta Path Microbiol Scand 1961; 52; 140-154.
Kumamoto H., Izutsu T., Ohki K., Takahashi N., Ooya K. p53 gene status and expression of p53, MDM2, and p14ARF proteins in ameloblastomas. Journal of Oral Pathology & Medicine.2004; 33, 5, pp. 292-299.
Slootweg PJ. P53 protein and ki-67 reactivity in epithelial odontogenic lesions. An immunohistochemical study. J Oral pathol Med 1995; 24; 393-397.
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