Hyaluronan does not improve bone healing in critical size calvarial defects in rats - a radiographic evaluation

Authors

  • Mariana Amade Mendes Brazão São Leopoldo Mandic Dental School
  • Beatriz de Brito Bezerra State University of Campinas
  • Márcio Zaffalon Casati State University of Campinas
  • Enilson Antônio Sallum State University of Campinas
  • Antônio Wilson Sallum State University of Campinas

DOI:

https://doi.org/10.20396/bjos.v9i2.8641899

Keywords:

Bone repair, Wound healing, Hyaluronan

Abstract

Aim: This study evaluated radiographically the effects of 1% hyaluronan in bone healing using a critical size rat-calvaria defect model. Methods: Thirty adult male Wistar rats were used in this study. Two 6-mm-diameter critical-size defects were created and the treatments were randomly distributed as follows: 1) 1% hyaluronan; 2) 1% hyaluronan soak loaded onto an absorbable collagen sponge (ACS) carrier; 3) saline; and 4) ACS alone. The animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs obtained using a direct digital radiograph system and a standardAim: This study evaluated radiographically the effects of 1% hyaluronan in bone healing using a critical size rat-calvaria defect model. Methods: Thirty adult male Wistar rats were used in this study. Two 6-mm-diameter critical-size defects were created and the treatments were randomly distributed as follows: 1) 1% hyaluronan; 2) 1% hyaluronan soak loaded onto an absorbable collagen sponge (ACS) carrier; 3) saline; and 4) ACS alone. The animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs obtained using a direct digital radiograph system and a standardized protocol. A blind examiner evaluated the radiographic density of the images twice and an intraclass correlation was performed to evaluate examiner reproducibility (R2=0.99, p<0.001). Comparisons between 4 and 8 weeks of treatment were performed by Student’s t test and comparisons between treatments and time by two-way ANOVA at 5% significance level. Results: There were no noteworthy differences between 4 or 8 weeks within each treatment group (p>0.05). When treatments were compared no significant differences between groups were found (p>0.05). Conclusions: Within the limits of this study, it can be concluded that 1% hyaluronan gel alone or its association with a carrier does not improve bone healing.ized protocol. A blind examiner evaluated the radiographic density of the images twice and an intraclass correlation was performed to evaluate examiner reproducibility (R2=0.99, p<0.001). Comparisons between 4 and 8 weeks of treatment were performed by Student’s t test and comparisons between treatments and time by two-way ANOVA at 5% significance level. Results: There were no noteworthy differences between 4 or 8 weeks within each treatment group (p>0.05). When treatments were compared no significant differences between groups were found (p>0.05). Conclusions: Within the limits of this study, it can be concluded that 1% hyaluronan gel alone or its association with a carrier does not improve bone healing.

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Author Biographies

Mariana Amade Mendes Brazão, São Leopoldo Mandic Dental School

DDS. Graduate Student, Department of Endodontics, São Leopoldo Mandic Dental School

Beatriz de Brito Bezerra, State University of Campinas

DDS, MS. Graduate Student, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas

Márcio Zaffalon Casati, State University of Campinas

DDS, MS, PhD. Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas

Enilson Antônio Sallum, State University of Campinas

DDS, MS, PhD. Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas

Antônio Wilson Sallum, State University of Campinas

DDS, PhD, Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas

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Published

2015-11-25

How to Cite

1.
Brazão MAM, Bezerra B de B, Casati MZ, Sallum EA, Sallum AW. Hyaluronan does not improve bone healing in critical size calvarial defects in rats - a radiographic evaluation. Braz. J. Oral Sci. [Internet]. 2015 Nov. 25 [cited 2022 Dec. 6];9(2):124-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641899

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