Hyaluronan does not improve bone healing in critical size calvarial defects in rats - a radiographic evaluation

  • Mariana Amade Mendes Brazão São Leopoldo Mandic Dental School
  • Beatriz de Brito Bezerra State University of Campinas
  • Márcio Zaffalon Casati State University of Campinas
  • Enilson Antônio Sallum State University of Campinas
  • Antônio Wilson Sallum State University of Campinas
Keywords: Bone repair, Wound healing, Hyaluronan

Abstract

Aim: This study evaluated radiographically the effects of 1% hyaluronan in bone healing using a critical size rat-calvaria defect model. Methods: Thirty adult male Wistar rats were used in this study. Two 6-mm-diameter critical-size defects were created and the treatments were randomly distributed as follows: 1) 1% hyaluronan; 2) 1% hyaluronan soak loaded onto an absorbable collagen sponge (ACS) carrier; 3) saline; and 4) ACS alone. The animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs obtained using a direct digital radiograph system and a standardAim: This study evaluated radiographically the effects of 1% hyaluronan in bone healing using a critical size rat-calvaria defect model. Methods: Thirty adult male Wistar rats were used in this study. Two 6-mm-diameter critical-size defects were created and the treatments were randomly distributed as follows: 1) 1% hyaluronan; 2) 1% hyaluronan soak loaded onto an absorbable collagen sponge (ACS) carrier; 3) saline; and 4) ACS alone. The animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs obtained using a direct digital radiograph system and a standardized protocol. A blind examiner evaluated the radiographic density of the images twice and an intraclass correlation was performed to evaluate examiner reproducibility (R2=0.99, p<0.001). Comparisons between 4 and 8 weeks of treatment were performed by Student’s t test and comparisons between treatments and time by two-way ANOVA at 5% significance level. Results: There were no noteworthy differences between 4 or 8 weeks within each treatment group (p>0.05). When treatments were compared no significant differences between groups were found (p>0.05). Conclusions: Within the limits of this study, it can be concluded that 1% hyaluronan gel alone or its association with a carrier does not improve bone healing.ized protocol. A blind examiner evaluated the radiographic density of the images twice and an intraclass correlation was performed to evaluate examiner reproducibility (R2=0.99, p<0.001). Comparisons between 4 and 8 weeks of treatment were performed by Student’s t test and comparisons between treatments and time by two-way ANOVA at 5% significance level. Results: There were no noteworthy differences between 4 or 8 weeks within each treatment group (p>0.05). When treatments were compared no significant differences between groups were found (p>0.05). Conclusions: Within the limits of this study, it can be concluded that 1% hyaluronan gel alone or its association with a carrier does not improve bone healing.

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Author Biographies

Mariana Amade Mendes Brazão, São Leopoldo Mandic Dental School
DDS. Graduate Student, Department of Endodontics, São Leopoldo Mandic Dental School
Beatriz de Brito Bezerra, State University of Campinas
DDS, MS. Graduate Student, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas
Márcio Zaffalon Casati, State University of Campinas
DDS, MS, PhD. Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas
Enilson Antônio Sallum, State University of Campinas
DDS, MS, PhD. Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas
Antônio Wilson Sallum, State University of Campinas
DDS, PhD, Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas
Published
2015-11-25
How to Cite
Brazão, M. A. M., Bezerra, B. de B., Casati, M. Z., Sallum, E. A., & Sallum, A. W. (2015). Hyaluronan does not improve bone healing in critical size calvarial defects in rats - a radiographic evaluation. Brazilian Journal of Oral Sciences, 9(2), 124-127. https://doi.org/10.20396/bjos.v9i2.8641899
Section
Article

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