Freshwater and salt-water influence in human identification by analysis of DNA: an epidemiologic and laboratory study

Authors

  • Jamilly de Oliveira Musse Universidade de São Paulo (USP), São Paulo (SP)
  • Amanda da Costa Nardis Faculdade de Odontologia, USP
  • Evelyn K. Anzai Universidade de São Paulo, USP
  • Mário H. Hirata Universidade de São Paulo
  • Regina Maria Barreto x Cicarelli Universidade de São Paulo
  • Rogério Nogueira de Oliveira Universidade de São Paulo

DOI:

https://doi.org/10.20396/bjos.v8i2.8642227

Keywords:

Human identification, Forensic dentistry, Teeth, DNA, Drowning

Abstract

Aim: To investigate the casuistry of drowning cases by reviewing the records from the Forensic Medicine Institute Nina Rodrigues in the city of Salvador, BA, Brazil, and to verify the potential of DNA recovery in human teeth immersed in water. Methods: An epidemiological survey was conducted followed by a laboratorial phase, in which 40 teeth were immersed in fresh and salt-water, the DNA was extracted by the organic method and amplified by polymerase chain reaction, using the amelogenin as initiator. The electrophoresis initially occurred in agarose gel and later in polyacrylamide gel. Results: In the present survey, 346 deaths from drowning were observed, most of them in salt-water (51.73%), with a predominance of male victims (86.13%) aged from 18 to 35 years-old (37.94%). Dentists identified 14.74% of the victims. DNA was recovered in 37.5% from the samples, most from teeth immersed in freshwater. Polyacrylamide gel analysis in samples that were amplified in agarose gel allowed correct gender identification in 83.3% of the cases. However, allele loss was observed in samples of two victims, jeopardizing gender determination. Conclusions: Dental exposure to water interfered in DNA recovery. The gender investigation using the amelogenin as initiator was effective.

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Author Biographies

Jamilly de Oliveira Musse, Universidade de São Paulo (USP), São Paulo (SP)

DDS, MSc, PhD Student in Community Dentistry, Faculdade de Odontologia, Universidade de São Paulo (USP), São Paulo (SP)

Amanda da Costa Nardis, Faculdade de Odontologia, USP

Undergraduate dental student, Faculdade de Odontologia, USP, São Paulo (SP)

Evelyn K. Anzai, Universidade de São Paulo, USP

PhD in Clinical Analysis, Faculdade de Ciências Farmacêuticas, USP, São Paulo (SP)

Mário H. Hirata, Universidade de São Paulo

DDS, MSc, PhD, Associate Professor, Faculdade de Ciências Farmacêuticas, USP, São Paulo (SP)

Regina Maria Barreto x Cicarelli, Universidade de São Paulo

DDS, MSc, PhD, Full Professor, Faculdade de Ciências Farmacêuticas, USP, São Paulo (SP)

Rogério Nogueira de Oliveira, Universidade de São Paulo

DDS, MSc, PhD, Professor, Faculdade de Odontologia, USP, São Paulo (SP)

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Published

2015-12-14

How to Cite

1.
Musse J de O, Nardis A da C, Anzai EK, Hirata MH, Cicarelli RMB x, Oliveira RN de. Freshwater and salt-water influence in human identification by analysis of DNA: an epidemiologic and laboratory study. Braz. J. Oral Sci. [Internet]. 2015 Dec. 14 [cited 2022 May 18];8(2):71-5. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642227

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