Enhanced susceptibility of Candida albicans to chlorhexidine under anoxia

  • Andressa Marafon Semprebom Pontifícia Universidade Católica do Paraná (PUCPR)
  • Ana Cláudia Azevedo Isidoro Pontifícia Universidade Católica do Paraná (PUCPR)
  • Maria Ângela Naval Machado Pontifícia Universidade Católica do Paraná (PUCPR)
  • Patrícia Maria Stuelp Campelo Pontifícia Universidade Católica do Paraná (PUCPR)
  • José Francisco Höfling Universidade Estadual de Campinas (Unicamp), Piracicaba (SP)
  • Lakshman Perera Samaranayake University of Hong Kong, Hong Kong, Hong Kong SAR
  • Edvaldo Antonio Ribeiro Rosa Pontifícia Universidade Católica do Paraná (PUCPR)
Keywords: Candida albicans, Chlorhexidine, Anoxia

Abstract

Aim: Periodontal pockets can be colonized not only by bacteria, but also by Candida albicans. However, its role in periodontitis is unknown. This study evaluated the inhibitory performance of chlorhexidine digluconate under normoxic and anoxic conditions against 16 strains of C. albicans from periodontal pockets and other 20 from the oral mucosa. Methods: Strains were grown in normoxia and anoxia to adapt themselves to the different atmospheric conditions. Microdilution-based assays were carried out to determine the minimum concentrations of chlorhexidine that may restrain the conditioned candidal strains, in normoxia (normoxic MIC) and anoxia (anoxic MIC). The Mann-Whitney U test was used to evaluate the antimicrobial effect of chlorhexidine on C. albicans under normoxic and anoxic conditions (α = 0.05). Results: The normoxic MIC of chlorhexidine varied broadly from 150 to 1200 µg/mL, whereas its anoxic MIC varied narrower from 2.34 to 37.5 µg/mL. Regarding the origins of strains, no statistically significant differences (p > 0.05) were found. Conclusions: These results indicate that anoxic environmental conditions, compatible with periodontal pockets, tend to enhance C. albicans susceptibility to chlorhexidine.

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Author Biographies

Andressa Marafon Semprebom, Pontifícia Universidade Católica do Paraná (PUCPR)
Laboratory of Stomatology, Dental School, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba (PR)
Ana Cláudia Azevedo Isidoro, Pontifícia Universidade Católica do Paraná (PUCPR)
Laboratory of Stomatology, Dental School, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba (PR)
Maria Ângela Naval Machado, Pontifícia Universidade Católica do Paraná (PUCPR)
Laboratory of Stomatology, Dental School, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba (PR)
Patrícia Maria Stuelp Campelo, Pontifícia Universidade Católica do Paraná (PUCPR)
Laboratory of Stomatology, Dental School, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba (PR)
José Francisco Höfling, Universidade Estadual de Campinas (Unicamp), Piracicaba (SP)
Laboratory of Microbiology and Immunology, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (Unicamp), Piracicaba (SP)
Lakshman Perera Samaranayake, University of Hong Kong, Hong Kong, Hong Kong SAR
Oral Biosciences Unity, Dental School, University of Hong Kong, Hong Kong, Hong Kong SAR
Edvaldo Antonio Ribeiro Rosa, Pontifícia Universidade Católica do Paraná (PUCPR)
Laboratory of Stomatology, Dental School, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba (PR)
Published
2015-12-14
How to Cite
Semprebom, A. M., Isidoro, A. C. A., Machado, M. Ângela N., Campelo, P. M. S., Höfling, J. F., Samaranayake, L. P., & Rosa, E. A. R. (2015). Enhanced susceptibility of Candida albicans to chlorhexidine under anoxia. Brazilian Journal of Oral Sciences, 8(2), 105-110. https://doi.org/10.20396/bjos.v8i2.8642264
Section
Article