Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal

Authors

  • Cristiane Duque Universidade Estadual de Campinas (Unicamp)
  • Thais de Cássia Negrini Unesp, Araraquara (SP)
  • Nancy Tomoko Sacono Unesp, Araraquara (SP)
  • Marcelo Fabiano Gomes Boriollo Universidade José do Rosário Vellano, Alfenas (MG)
  • José Francisco Hofling Faculdade de Odontologia de Piracicaba, Unicamp, Piracicaba (SP)
  • Josimeri Hebling Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)
  • Denise Madalena Palomari Spolidorio Unesp, Araraquara (SP)

DOI:

https://doi.org/10.20396/bjos.v8i1.8642286

Keywords:

Streptococcus mutans, Polymerase chain reaction, Polymorphism, Genetic, Dental caries

Abstract

Aim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of Smutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment.

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Author Biographies

Cristiane Duque, Universidade Estadual de Campinas (Unicamp)

DDS, PhD, Post-doctorate student, Department of Microbiology and Immunology, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (Unicamp), Piracicaba (SP), Brazil; Department of Orthodontics and Pediatric Dentistry, Faculdade de Odontologia de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho” (Unesp), Araraquara (SP),

Thais de Cássia Negrini, Unesp, Araraquara (SP)

DDS, MS, Doctorate Student; Department of Clinical Analysis, Faculdade de Ciências Farmacêuticas, Unesp, Araraquara (SP)

Nancy Tomoko Sacono, Unesp, Araraquara (SP)

DDS, MS, Doctorate Student, Department of Orthodontics and Pediatric Dentistry, Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)

Marcelo Fabiano Gomes Boriollo, Universidade José do Rosário Vellano, Alfenas (MG)

DDS, MS, PhD, Professor, Department of Microbiology, Universidade José do Rosário Vellano, Alfenas (MG)

José Francisco Hofling, Faculdade de Odontologia de Piracicaba, Unicamp, Piracicaba (SP)

BSc, PhD, Professor, Department of Microbiology and Immunology, Faculdade de Odontologia de Piracicaba, Unicamp, Piracicaba (SP)

Josimeri Hebling, Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)

DDS, PhD, Professor, Department of Orthodontics and Pediatric Dentistry, Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)

Denise Madalena Palomari Spolidorio, Unesp, Araraquara (SP)

BSc, PhD, Professor, Department of Physiology and Pathology, Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)

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Published

2015-12-17

How to Cite

1.
Duque C, Negrini T de C, Sacono NT, Boriollo MFG, Hofling JF, Hebling J, Spolidorio DMP. Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal. Braz. J. Oral Sci. [Internet]. 2015Dec.17 [cited 2021Oct.24];8(1):2-8. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642286

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