The effects of age and gender on CD3+ and CD19+ lymphocyte in gingival tissue and peripheral blood: an animal study

Authors

  • Turgut Demir Atatürk University
  • Varol Canakci Atatürk University
  • Cankat Kara Atatürk University
  • Cenk Fatih Canakci Atatürk University
  • Fuat Erdem Atatürk University
  • Mustafa Atasever

DOI:

https://doi.org/10.20396/bjos.v7i26.8642744

Keywords:

Lymphocytes, CD3 , CD19 , Age, Gender, Gingiva, Peripheral blood, Mice

Abstract

Aim: The aim of the present study was to determine age- and gender-related values for healthy mice of CD3+ T and CD19+ B lymphocytes and CD3+/ CD19+ (T/B) ratios in peripheral blood and gingival tissue by the flow cytometry technique. Methods: The study was carried out on periodontally healthy 60 BALB/c mice. They were divided into five groups according to the their age (newborn, weaning, puberty, adult and elder). Males and females were equally represented in each group. CD3+ and CD19+ T lymphocytes and CD3+/CD19+ ratio values in gingival tissue and peripheral blood were determined using flow cytometry in the biopsy samples. Results: There were no significant differences in the CD3+ and CD19+ lymphocytes, and CD3+/ CD19+ ratios in gingival tissue for all age groups (p>0.05). Mean relative number of peripheral blood CD3+ T lymphocyte indicated a decrease in puberty group compared to the other groups (p<0.05), while relative number of peripheral blood CD19+ B lymphocyte increased in adult and aged group. The peripheral blood CD3+/ CD19+ T/B lymphocytes ratios decreased in adult and aged group. Conclusions: The results of the present study showed that differences were present in periods of life and gender in peripheral blood of mice. Moreover, significantly differences were found between genders in gingival tissue.

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Author Biographies

Turgut Demir, Atatürk University

Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey

Varol Canakci, Atatürk University

Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey

Cankat Kara, Atatürk University

Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey

Cenk Fatih Canakci, Atatürk University

Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey

Fuat Erdem, Atatürk University

Department of Internal Medicine, Atatürk University, Faculty of Medicine, Erzurum, Turkey

Mustafa Atasever

Department of “Food Science and Technology”, Atatürk University, Faculty of Veterinary Science, Erzurum, Turkey

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Published

2016-01-08

How to Cite

1.
Demir T, Canakci V, Kara C, Canakci CF, Erdem F, Atasever M. The effects of age and gender on CD3+ and CD19+ lymphocyte in gingival tissue and peripheral blood: an animal study. Braz. J. Oral Sci. [Internet]. 2016 Jan. 8 [cited 2022 Dec. 3];7(26):1609-13. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642744

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