The effects of age and gender on CD3+ and CD19+ lymphocyte in gingival tissue and peripheral blood: an animal study

  • Turgut Demir Atatürk University
  • Varol Canakci Atatürk University
  • Cankat Kara Atatürk University
  • Cenk Fatih Canakci Atatürk University
  • Fuat Erdem Atatürk University
  • Mustafa Atasever
Keywords: Lymphocytes, CD3 , CD19 , Age, Gender, Gingiva, Peripheral blood, Mice

Abstract

Aim: The aim of the present study was to determine age- and gender-related values for healthy mice of CD3+ T and CD19+ B lymphocytes and CD3+/ CD19+ (T/B) ratios in peripheral blood and gingival tissue by the flow cytometry technique. Methods: The study was carried out on periodontally healthy 60 BALB/c mice. They were divided into five groups according to the their age (newborn, weaning, puberty, adult and elder). Males and females were equally represented in each group. CD3+ and CD19+ T lymphocytes and CD3+/CD19+ ratio values in gingival tissue and peripheral blood were determined using flow cytometry in the biopsy samples. Results: There were no significant differences in the CD3+ and CD19+ lymphocytes, and CD3+/ CD19+ ratios in gingival tissue for all age groups (p>0.05). Mean relative number of peripheral blood CD3+ T lymphocyte indicated a decrease in puberty group compared to the other groups (p<0.05), while relative number of peripheral blood CD19+ B lymphocyte increased in adult and aged group. The peripheral blood CD3+/ CD19+ T/B lymphocytes ratios decreased in adult and aged group. Conclusions: The results of the present study showed that differences were present in periods of life and gender in peripheral blood of mice. Moreover, significantly differences were found between genders in gingival tissue.

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Author Biographies

Turgut Demir, Atatürk University
Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey
Varol Canakci, Atatürk University
Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey
Cankat Kara, Atatürk University
Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey
Cenk Fatih Canakci, Atatürk University
Department of Periodontology, Atatürk University, Faculty of Dentistry, Erzurum, Turkey
Fuat Erdem, Atatürk University
Department of Internal Medicine, Atatürk University, Faculty of Medicine, Erzurum, Turkey
Mustafa Atasever
Department of “Food Science and Technology”, Atatürk University, Faculty of Veterinary Science, Erzurum, Turkey
Published
2016-01-08
How to Cite
Demir, T., Canakci, V., Kara, C., Canakci, C. F., Erdem, F., & Atasever, M. (2016). The effects of age and gender on CD3+ and CD19+ lymphocyte in gingival tissue and peripheral blood: an animal study. Brazilian Journal of Oral Sciences, 7(26), 1609-1613. https://doi.org/10.20396/bjos.v7i26.8642744
Section
Article