Evaluation of root structure loss using AutoCAD assisted histomorphometry

Abstract

Aims: The quantitative measurement and characterization of microscopical images using a computer is histomorphometry. The purpose of this experimental research is to evaluate the validity of two methods of histomorphometry of root structure loss measuring antiresorptive efficacy of topical application of alendronate in delayed tooth replantation on a canine model. Methods: We used fortyeight premolar mature roots from five dogs in the study. Roots were soaked in: 1 mMol of ALN, 3 mMol of ALN and physiological saline. After 4 months, animals were sacrificed and a bone block was prepared for analysis. Histological slides were photographed with digital camera (Olympus C 5060 5.1 megapixel) which is directly attached to microscope (Carl Zeiss, Axiolab,Jena, Germany) connected to a PC. The images obtained were stored as figures (TIFF) for further interpretation. Root structure loss due to resorption was measured in two ways. Results: The t-test for two independent samples for evaluating root mass loss between different methods of measurement was used. (t=4.497; df=17; p<0.05). Conclusion: The obtained results showed additional accuracy and precision by computer method that enables future researchers in the area of traumatic injuries of dento-alveolar system, for applying in pathohistology scientific approach, and for measuring the resorption changes on the roots of replanted teeth.