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Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population
Remote (Português (Brasil))


Periapical granuloma. Enterococcus faecalis. Enterococcus

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Sánchez-Sanhueza G, González-Rocha G, Bello-Toledo H. Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population. Braz. J. Oral Sci. [Internet]. 2015 Jan. 1 [cited 2023 Oct. 3];14(3):240-5. Available from:


Aim: To isolate and identify in a Chilean population, Enterococcus spp. from root canals with persistent chronic apical periodontitis (CAP) and to investigate the potential correlation between the bacteria and the observed clinical features. Methods: Twenty patients with indication for endodontic retreatment due to persistent CAP were selected. Data from patient general health and dental clinical history were recorded. During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. The relationship between the number of colony-forming units of Enterococcus spp. and patient clinical data was assessed statistically by the Pearson Chi square and Fisher exact tests. Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. was conducted in the clinical samples, and the results were analyzed by a proportion comparison test. Results: Enterococcus spp. strains were isolated in 70% of the patients. Most of them (98.8%) accounted for Enterococcus faecalis and only 1.2% for Enterococcus faecium. A high frequency of E. faecalis was found in teeth with inadequate endodontic treatment or dental crown restorations. Conclusions: This study concluded that E. faecalis is prevalent in root canals with persistent CAP in a Chilean population. E. faecium as found in a single case with the poorest root canal filling. Further studies are still required to investigate the presence of other species, which may be linked to persistent chronic apical periodontitis.


Remote (Português (Brasil))


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Copyright (c) 2015 Gabriela Sánchez-Sanhueza, Gerardo González-Rocha, Helia Bello-Toledo


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