Antimicrobial activity of coffee-based solutions and their effects on Streptococcus mutans adherence

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DOI:

https://doi.org/10.20396/bjos.v6i20.8642964

Keywords:

Streptococcus mutans. Coffea arabica

Abstract

The aim of this study was to evaluate the antimicrobial activity of different coffee solutions and their effects on the adherence of Streptococcus mutans to glass surface. Coffee solutions were prepared with three commercial products (Pilão, Mellita and Café do Ponto) by two different methods (simple and boiled) (n=15). A control group was also included in the study. For antimicrobial activity testing, tubes containing coffee solution and culture medium were inoculated with a suspension of S. mutans ATCC 35688 and incubated for 1min, 1h, 2h and 4h. Serial dilutions and plating on BHI agar were performed. S. mutans adherence to glass in presence of the different coffee solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method. The results were statistically analyzed by ANOVA and Tukey’s test. The tested coffee solutions did not reduce the number of colony forming units of S. mutans in relation to the control at all evaluation periods. All the solutions reduced significantly the adherence of S. mutans to the glass surface in relation to control. The tested coffee solutions did not present any antimicrobial effect on Streptococcus mutans, however, all the coffee solutions reduced significantly the adherence of S. mutans to the glass surface.

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Author Biographies

Eloiza Helena da Silva Brandão, São Paulo State University

Possui graduação em odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho(2004). Tem experiência na área de Odontologia.

Luciane Dias Oliveira, São Paulo State University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP (1998), Mestrado em Biopatologia Bucal (2002) UNESP, Doutorado em Biopatologia Bucal (2005) pela UNESP e Pós-Doutorado em Biopatologia Bucal (2009) pela UNESP, todos com bolsa FAPESP.

Cristiane Yumi Koga-Ito, São Paulo State University

Possui graduação em Odontologia pela UNESP, S. Jose dos Campos (1993), mestrado (1995) e doutorado em Biologia Patologia Buco-Dental pela UNICAMP (1997). Realizou pós-doutorado na Universidade de Turim- Itália (1997-1999) na área de Micologia Médica e na Universidade Federal de Minas Gerais na área de Micologia e Biologia Molecular (2000-2002).

Antonio Olavo Cardoso Jorge, São Paulo State University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1977), Especialização em Biologia e em Endodontia, Mestrado em Biologia (Fisiopatologia Celular) pela Universidade de Taubaté (1986), mestrado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1988), Doutorado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1991) e Livre-Docência em Microbiologia e Imunologia pela Faculdade de Odontologia de São José dos Campos/UNESP (1995).

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Published

2016-01-20

How to Cite

1.
Brandão EH da S, Oliveira LD, Landucci LF, Koga-Ito CY, Jorge AOC. Antimicrobial activity of coffee-based solutions and their effects on Streptococcus mutans adherence. Braz. J. Oral Sci. [Internet]. 2016 Jan. 20 [cited 2023 Feb. 2];6(20):1274-7. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642964

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