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Water from dental equipment presents risks for surgeon-dentists as well as for patients because it might work as a means of dissemination/ transmission of microorganisms. The objective of this study was to verify the quality of the water used in dental equipment by means of microbiological analysis, accomplishing the count of Staphylococcus spp. There have been collected 160 samples of water from reservoirs, taps used for hand washing, air-water syringes, and high-speed handpieces, in 40 dental offices in the city of Barretos, São Paulo. The rules concerning bacteriological analysis in cfu/mL from Standard Methods for the Examination of Water and Wastewater have been followed. The analysis of the results has made it possible to verify that out of the total of samples, 28% did not meet the standards of potability established by the American Dental Association. Regarding the origin of analyzed S. aureus, the most contaminated sites were high-speed handpieces in private offices (76%) and in dental care plan offices (71%), followed by air-water syringe in dental care plan offices (64%). For S. epidermidis samples, the most contaminated sites were high-speed handpieces in SUS (Brazilian Government Health System) dental offices (22%) and in dental care plan offices (14%). The most contaminated sites were dental offices that saw patients under dental care plans. Concerning tested antibiotics, the ones that presented better results as to sensibility to strain S. epidermidis were vancomycin and ciprofloxacin (100%) and, as to sensibility to strain S. aureus, it was ciprofloxacin (97%)References
Agostinho AM, Watanabe E, Matsumoto W, Ito IY. Dental unit water: the effect of flushing in old and new dental units. J Dent Res. 2003; 82: 79.
Mills SE. Waterborne pathogens and waterline. Dent Clin North Am. 2003; 47: 545-57.
Barbeau J. Waterborne biofilms and dentistry: the changing face of infection control. J Can Dent Assoc. 2000; 66: 539-41.
Fiehn NE, Larsen T. The effect of drying dental unit waterline biofilms on the bacterial load of dental unit water. Int Dent J. 2002; 52: 251-54.
American Public Health Association. American Water Works Association. Water Environment Federation. Standard methods for examination of water and wastewater. 20th ed. Washington: APHA; 1999. 9.29-9.30.
National Committee for Clinical Laboratory Standards.
Approved Standard M2-A7, 1995. Performance standard for antimicrobial disk susceptibility test, vol. 15, n. 14, Approved Standard NCCLS, Villanova, Pa.
S.A.S. INSTITUTE INC. S.A.S/Stat – User’s guide, release 6, 12 TS level 0020. Cary. 1999. p.519-48.
Watanabe E. Avaliação do nível de contaminação da água de equipo odontológico [thesis]. Ribeirão Preto: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP; 2003. 113p.
American Dental Association. ADA statement on dental unit waterlines. July 2004. Available from: http://www.ada.org/prof/ resources/positions/statments/lines.asp [2003 Sept 16].
Cardoso ML, Vicente VA, Ferreira EL, Carneiro E. Qualidade microbiológica da água utilizada em turbinas de alta rotação em três condições clínicas diferentes. Rev Assoc Paul Cir Dent. 1999; 53: 387-93.
Aguiar CM, Pinheiro JT. Avaliação bacteriológica da qualidade de água utilizada nos equipos odontológicos. Rev. Assoc Paul Cir Dent. 1999; 53: 228-35.
Kohno S, Kawata T, Kaku M, Fuita T, Tsutsui K, Ohtani J et al. Bactericidal effects of acidic electrolyzed water on dental unit waterline. Jpn J Infect Dis. 2004; 57: 52-4.
Prevost AP, Robert M, Charland R, Barbeau J. Doctor, Would you drink water from your dental unit? N Y State Dent J. 1995; 61: 22-8.
Williams HN, Kelley J, Folineo D, Williams GC, Hawley CL, Sibiski J. Assessing microbial contamination in clean water dental units and compliance with disinfection protocol. J Am Dent Assoc. 1994; 125: 1205-11.
Aires De Sousa M, Crisostomo MI, Santos Sanches I, WU JS, Fuzhong J, Tomasz A, De Lencastre H. Frequent recovery of a single clonal type of multidrug-resistant Staphylococcus aureus from patients in two hospitals in Taiwan and China. J Clin Microbiol. 2003, 41: 159-63.
Miragaia M, Couto I, Pereira Sandro FF, Sandro FF, Kristinsson KG, Westh H et al. Molecular characterization of methicillinresistant Staphylococcus epidermidis: evidence of geographic dissemination. J Clin Microbiol. 2003; 40: 430-8.
Chang MR, Carvalho NCP, Oliveira ALL, Moncada PMF, Moraes BA et al. Surveillance of pediatric infections in a teaching hospital in Mato Grosso do Sul, Brazil. Braz J Infect Dis. 2003; 7: 149-60.
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