Effect of blood contamination during adhesive restorative procedures on dentin-resin cement shear bond strength

Authors

  • Carlos José Soares Federal University of Uberlândia.
  • Carolina Assaf Branco Federal University of Uberlândia
  • Priscilla Barbosa Ferreira Soares Federal University of Uberlândia
  • Rodrigo Borges Fonseca Federal University of Uberlândia
  • Hugo Lemes Carlo Federal University of Uberlândia http://orcid.org/0000-0002-5111-7781
  • Alfredo Júlio Fernandes Neto Federal University of Uberlândia

DOI:

https://doi.org/10.20396/bjos.v6i21.8642972

Keywords:

Blood contamination. Adhesive system. Dentin bonding. Indirect restoration. Shear bond strength

Abstract

The aim of this study was to evaluate the effect of dentin blood contamination during different steps of the restorative procedure on resin cement shear bond strength to dentin. Crows of 120 bovine incisors were prepared to obtain flat superficial dentin surfaces. Dentin was etched with phosphoric acid and contaminated with fresh blood for 10 seconds, before or after adhesive system application. Different treatments were tested in contaminated dentin, resulting on eight groups (N=15). Composite resin restorations (TPH Spectrum, Dentsply) were adhesively fixed (Excite, Ivoclar-Vivadent) with resin cement (Variolink 2, Ivoclar-Vivadent) and shear bond strength test (0.5 mm/min) was performed. Morphologic observations were carried out with scanning electron microscopy (SEM). Data (MPa) were submitted to one-way ANOVA following Tukey’s test (p<0.05), showing that blood contamination during adhesive procedure negatively affects bond strength, and decontamination methods do not recover original bond strength. The negative effects of blood contamination on shear bond strength to dentin and resin cement were significant in all contaminated groups; none of the tested dentin treatment procedures resulted in higher bond strength irrespective of the moment on which blood contamination took place.

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Author Biographies

Carlos José Soares, Federal University of Uberlândia.

Professor Adjunto 3 da Faculdade de Odontologia da UFU, atualmente exerce o cargo de Coordenador de Área da Odontologia na CAPES e é membro da Câmara da Saúde na FAPEMIG. Fellow da Academy of Dental Materials ? ADM. Possui graduação em Odontologia pela Universidade Federal de Uberlândia - UFU (1991), Especialização em Dentística pela UFU (1994), mestrado em Clínica Odontológica - Área de Dentística pela Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas - FOP/UNICAMP (2000) e doutorado em Clínica Odontológica - Área de Dentística pela FOP/UNICAMP (2003), Pos-Doutorado na University of Minnesota (2009) no desenvolvimento de modelos de elementos finitos em multi-escala. 

Carolina Assaf Branco, Federal University of Uberlândia

Graduação em Odontologia pela Universidade Federal de Uberlândia (2004) e é formada em Acupuntura pelo Instituto Brasileiro de Estudos Holísticos (2004). Mestrado em Odontologia pela Universidade Federal de Uberlândia. Especialização em Dentistica Restauradora pela Universidade Federal de Uberlândia. Atualmente é Doutoranda em Odontologia Restauradora na Faculdade de Odontologia de Ribeirão Preto - USP. Tem experiência na área de Odontologia, com ênfase em Clínica Odontológica, atuando principalmente nas seguintes áreas: Dentística Restauradora, Reabilitação Oral, Materiais Odontológicos, Dor Orofacial e Acupuntura.

Priscilla Barbosa Ferreira Soares, Federal University of Uberlândia

Possui graduação em Odontologia pela Universidade Federal de Uberlândia (1998), Aperfeiçoamento em Implantodontia pela UFU (2001), Especialização em Periodontia pela Universidade Federal de Uberlândia (2000) e Mestrado em Reabilitação Oral pela UFU (2008). Atua como professor na área de Periodontia e Implantodontia da Faculdade de Odontologia Universidade Federal de Uberlândia.

Rodrigo Borges Fonseca, Federal University of Uberlândia

Professor Adjunto DE, Coordenador de Pesquisa da FO-UFG, Líder da Rede Goiana de Pesquisa em Biologia Oral, Biomateriais, Biomecânica e Ensaios Clínicos em Odontologia. Vice-coordenador do Programa de Mestrado em Odontologia da UFG (2012-2014)

Hugo Lemes Carlo, Federal University of Uberlândia

Professor Adjunto na Faculdade de Odontologia da Universidade Federal de Juiz de Fora - Campus Governador Valadares. Graduado em Odontologia pela Faculdade de Odontologia da Universidade Federal de Uberlândia (1996/2000). Especialista em Dentística Restauradora pela Faculdade de Odontologia da Universidade Federal de Uberlândia (2001/2002).

Alfredo Júlio Fernandes Neto, Federal University of Uberlândia

Alfredo Julio Fernandes Neto, Concluiu Graduação em Odontologia pela Universidade Federal de Uberlândia - UFU em 1975, Mestrado em Odontologia (Reabilitação Oral) pela Faculdade de Odontologia de Bauru da Universidade de São Paulo em 1982, Doutorado em Odontologia (Reabilitação Oral) pela Faculdade de Odontologia de Ribeirão Preto da Universidade de São Paulo em 1996

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Published

2016-01-21

How to Cite

1.
Soares CJ, Branco CA, Soares PBF, Fonseca RB, Carlo HL, Fernandes Neto AJ. Effect of blood contamination during adhesive restorative procedures on dentin-resin cement shear bond strength. Braz. J. Oral Sci. [Internet]. 2016Jan.21 [cited 2021Nov.27];6(21):1320-5. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642972

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