Banner Portal
A new method for extraction of mandibular first molars in rats


Tooth extraction. Mandibular molar. Wound healing. Rats

How to Cite

Zecchin KG, Jorge R da S, Jorge J. A new method for extraction of mandibular first molars in rats. Braz. J. Oral Sci. [Internet]. 2016 Jan. 21 [cited 2024 Apr. 19];6(21):1344-8. Available from:


The aim of the present study is to describe a new technique for the mandibular first molar extraction based on tooth section in rats. One hundred and forty Wistar rats, from three different researches underwent general anaesthesia. Each animal was then positioned on a specific board and hollemback 3ss was used to make the syndesmotomy. The mandibular first molars were extracted after tooth section with carbide ¼ drills in a high-speed hand piece under constant irrigation with sterile saline solution. The mesial portion of the first molar was removed with a modified curved mosquito forceps. The distal portion was removed and the socket was closed with 5-0 nylon thread sutures using non-traumatic needles. During the first week after tooth extraction, animals were fed with regular pressed food to avoid post-operatory trauma. This technique is careful and avoid unnecessary trauma, with minimal numbers of fractures (9.3%) and accidents such as haemorrhage (2%). All the reminiscent roots could be removed by the described method. Our technique proved to be an efficient model for future researches on alveolar wound healing with minimal fractures and accidents and provided better post-extraction outcomes for the rats.


Amler MH, Johnson PL, Salman I. Histological and histochemical investigation of human alveolar socket healing in undisturbed extraction wounds. J Am Dent Assoc. 1960; 61: 32-44.

Boyne PJ. Osseous repair of the postextraction alveolus in man.

Oral Surg Oral Med Oral Pathol. 1966; 21: 805-13.

Becker W, Becker BE, Caffesse R. A comparison of demineralized freeze-dried bone and autologous bone to induce bone formation in human extraction sockets. J Periodontol. 1994; 5: 1128-33.

Moller JF, Petersen JK. Efficacy of a fibrin sealant on healing of extraction wounds. Int J Oral Maxillofac Surg. 1988; 17: 142-4.

Marmary Y, Brayer L, Tzukert A, Feller L. Alveolar bone repair following extraction of impacted mandibular third molars. Oral Surg Oral Med Oral Pathol. 1986; 61: 324-6.

Braegger U, Litch J, Pasquali L, Komman KS. Computer assisted densitometric image analysis for the quantitation of radiographic alveolar bone changes. J Periodontal Res. 1987; 22: 227-9.

Carvalho TL, Araujo CA, Teofilo JM, Brentegani LG. Histologic and histometric evaluation of rat alveolar wound healing around polyurethane resin implants. Int J Oral Maxillofac Surg. 1997; 26: 149-52.

McMillan MD. Neutrophils in the molar tooth extraction wound in the rat: a transmission electron microscope (TEM) study. J Oral Pathol Med. 1999; 28: 297-302.

Devlin H. Early bone healing events following rat molar tooth extraction. Cells Tissues Organs. 2000; 167: 33-7.

Brentegani LG, Bombonato KF, Carvalho TL. Immediate implantation of glass-ionomer cement granules increases osteogenesis during rat alveolar wound healing. J Nihon Univ Sch Dent. 1996; 38: 141-5.

Lekic P, Rubbino I, Krasnoshtein F, Cheifetz S, McCulloch CA, Tenenbaum H. Bisphosphonate modulates proliferation and differentiation of rat periodontal ligament cells during wound healing. Anat Rec. 1997; 247: 329-40.

Silva HC, Coletta RD, Jorge J, Bolzani G, Almeida OP, Graner E.

The effect of cyclosporin A on the activity of matrix metalloproteinases during the healing of rat molar extraction wounds. Arch Oral Biol. 2001; 46: 875-9.

Shimizu M, Furuya R, Kawawa T, Sasaki T. Bone wound healing after maxillary molar extraction in ovariectomized aged rats: quantitative backscattered electron image analysis. Anat Rec.

; 259: 76-85.

Zecchin KG, Pereira MC, Coletta RD, Graner E, Jorje J.

Ovariectomy reduces the gelatinolytic activity and expression of matrix metalloproteinases and collagen in rat molar extraction wounds Calcif Tissue Int. 2005; 76: 136-45.

Pereira MC, Zecchin KG, Campagnoli EB, et al. Ovariectomy delays alveolar wound healing after molar extractions in rats. J Oral Maxillofac Surg. [in press] (accepted in Dec 7th, 2006).

Devlin H, Garland H, Sloan P. Healing of tooth extraction sockets in experimental diabetes mellitus. J Oral Maxillofac Surg. 1996; 54: 1087-91.

Boyes-Varley JG, Cleaton-Jones PE, Lownie JF. Effect of a topical drug combination on the early healing of extraction sockets in the vervet monkey. Int J Oral Maxillofac Surg. 1988; 17: 138- 41.

Freitag V, Landau H. Histological findings in the alveolar sockets and tooth roots after experimental mandibular fractures in dogs.

J Craniomaxillofac Surg. 1997; 25: 203-11.

Mason AG, Holland GR. The reinnervation of healing extraction sockets in the ferret. J Dent Res. 1993; 72: 1215-21.

Kuboki Y, Hashimoto F, Ishibashi K. Time-dependent changes of collagen crosslinks in the socket after tooth extraction in rabbits. J Dent Res. 1988; 67: 9448.

Tanaka S, Shimizu M, Debari K, Furuya R, Kawawa T, Sasaki T.

Acute effects of ovariectomy on wound healing of alveolar bone after maxillary molar extraction in aged rats. Anat Rec.

; 262: 203-12.

Okamoto T, Gabrielli MF, Gabrielli MA. Influence of different types of non-resorbable suture material on the healing of extraction wounds-a histological study in rats. J Nihon Univ Sch Dent. 1990; 32: 104-15.

Okamoto T, Okamoto R, Alves Rezende MC, Gabielli MF.

Interference of the blood clot on granulation tissue formation after tooth extraction. Histomorphological study in rats. Braz Dent J. 1994; 5: 85-92.

de Carvalho PS, Mariano RC, Okamoto T. Treatment of fibrinolytic alveolitis with rifamycin B diethylamide associated with gelfoam: a histological study. Braz Dent J. 1997; 8: 3-8.

Elovic RP, Hipp JA, Hayes WC. Maxillary molar extraction decreases stiffness of the mandible in ovariectomized rats. J Dent Res. 1994; 73: 1735-41.

Li X, Nishimura I. Altered bone remodeling pattern of the residual ridge in ovariectomized rats. J Prosthet Dent. 1994; 72: 324- 30.

Smith N. A comparative histological and radiographic study of extraction socket healing in the rat. Aust Dent J. 1974; 19: 250-4.

Bodner L, Dayan D, Rothchild D, Hammel I. Extraction wound healing in desalivated rats. J Oral Pathol Med. 1991; 20: 176-8.

Iizuka T, Miller SC, Marks SC, Jr. Alveolar bone remodeling after tooth extraction in normal and osteopetrotic (ia) rats. J Oral Pathol Med. 1992; 21: 150-5.

Bodner L, Kaffe I, Cohen Z, Dayan D. Long-term effect of desalivation on extraction wound healing: a densitometric study in rats. Dentomaxillofac Radiol. 1993; 22: 195-8.

Guglielmotti MB, Cabrini RL. Alveolar wound healing and ridge remodeling after tooth extraction in the rat: a histologic, radiographic, and histometric study. J Oral Maxillofac Surg. 1985; 3: 359-64.

Vignery A, Baron R. Dynamic histomorphometry of alveolar bone remodeling in the adult rat. Anat Rec. 1980; 96: 191-200.

Hsieh YD, Devlin H, McCord F. The effect of ovariectomy on the healing tooth socket of the rat. Arch Oral Biol. 1995; 40: 529-31.

Doku HC, Shklar G, Bugbee B. The effect of epsilon aminocaproic acid on the healing of extraction wounds in hamsters. Oral Surg Oral Med Oral Pathol. 1966; 2: 569-77.

Dayan D, Bodner L, Horowitz I. Effect of salivary gland hypofunction on the healing of extraction wounds: a histomorphometric study in rats. J Oral Maxillofac Surg. 1992; 50: 354-8.

Bodner L, Kaffe I, Littner MM, Cohen J. Extraction site healing in rats. A radiologic densitometric study. Oral Surg Oral Med Oral Pathol. 1993; 75: 367-72.

Ubios AM, Jares FG, Guglielmotti MB. Effect of calcitonin on alveolar wound healing. J Oral Pathol Med. 1991; 20: 322-4.

Carvalho TL, Teofilo JM, Araujo CA, Brentegani LG. Chronology of alveolar healing following immediate implantation of Ricinus communis polyurethane resin: histometric analysis in rats. J Biomed Mater Res. 1997; 37: 449-52.

Carvalho A, Castro AL, Santos Pinto MC. Mineralization in the repair process of tooth extraction lesions in rats. Contribution to densitometric study. Ars Curandi Odontol. 1980; 7: 304-12.

Okamoto T, Vasconcelos Fialho AC. Comparative histological study of two methods of obtaining alveolar sections in rats. Rev Odontol UNESP. 1990; 19: 63-74.

Nishimura I, Damiani PJ, Atwood DA. Resorption of residual ridges (RRR) in rats. J Dent Res. 1987; 66: 1753-7.

Pietrokovski J, Massler M. Alveolar ridge resorption following tooth extraction. J Prosthet Dent. 1967; 17: 21-7.

The Brazilian Journal of Oral Sciences uses the Creative Commons license (CC), thus preserving the integrity of the articles in an open access environment.


Download data is not yet available.