An in vitro microbial model for producing caries-like lesions on enamel


  • Carolina Steiner-Oliveira State University of Campinas
  • Fernando A. Maciel Faculty of Medical Sciences,
  • Lidiany K.A. Rodrigues Nursing and Dentistry of Federal University of Ceará
  • Marcelo H. Napimoga State University of Campinas
  • Luiz A.F. Pimenta State University of Campinas
  • José F. Höfling State University of Campinas
  • Reginaldo B. Gonçalves State University of Campinas



Biofilms. Microbiological. Enamel. S. mutans. S. sobrinus


This study aimed to develop a low cost in vitro viable microbiological model to produce biofilms to be used in dental researches. Single and multi-species biofilms of S. mutans, S. sobrinus, S. mitis, S. salivarius, S. cricetus and S. sanguinis were grown on bovine enamel slabs during 10 days, in a sterile brain-heart infusion broth, containing 5% sucrose and incubated at 37ºC in an atmosphere of 10% CO2 . The slabs were transferred to a fresh medium at every 6, 12 or 24 hours. After the experimental period, enamel volume percent mineral was determined by cross-sectional microhardness. Caries-like lesions were found in all bacterial groups when compared with the control group. No statistical significant differences were found between S. mutans and S. sobrinus with respect of their cariogenicity or among the periods of medium change. However, it was found a statistical significant difference among the cariogenicity of S. salivarius and S. sanguinis (ANOVA followed by Tukey test). This model has successfully developed caries-like lesion on enamel and the medium can be changed at every 24 hours utilizing either S. mutans or S. sobrinus.


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Author Biographies

Carolina Steiner-Oliveira, State University of Campinas

Professora da Faculdade de Odontologia de Piracicaba, UNICAMP, área de Odontopediatria. Possui graduação em Odontologia pela Universidade Estadual de Campinas (2003), mestrado em Odontologia pela Universidade Estadual de Campinas (2006), doutorado em Odontologia - Odontopediatria pela Universidade Estadual de Campinas com estágio sanduíche na Indiana University, US e Especialista em Ortodontia e Ortopedia Funcionial pela Associação Paulista de Cirurgiões-Dentistas.

Marcelo H. Napimoga, State University of Campinas

Possui graduação em Odontologia pela Universidade Estadual de Campinas (2002), mestrado em Cariologia pela Universidade Estadual de Campinas (2004) e doutorado em Microbiologia e Imunologia no Instituto Forsyth - Harvard Medical School (Boston) e pela Universidade Estadual de Campinas (2005) e pós doutor em Imunofarmacologia pela Faculdade de Medicina de Ribeirão Preto - USP (2006).

José F. Höfling, State University of Campinas

Atualmente é Professor Titular da Faculdade de Odontologia de Piracicaba, da Universidade Estadual de Campinas. Formou-se em Licenciatura e Bacharelado em Historia Natural pela Faculdade de Filosofia, Ciencias e Letras de Rio Claro, hoje UNESP "campus" de Rio Claro, em 1970. Desde 1967, quando ainda aluno, foi bolsista da Fapesp ( iniciaçao cientifica) durante todo o periodo de graduacão.

Reginaldo B. Gonçalves, State University of Campinas

Professor Titular de microbiologia e imunologia da Faculdade de Odontologia da Universidade Estadual de Campinas e professor associado em Periodontia da Faculté de Medecine Dentaire, Université Laval, Québec, Canada. Possui graduação em Odontologia pela Universidade Estadual de Campinas (1987), especialização em Endodontia pela FOP-Unicamp (1989), mestrado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1994) e doutorado em Ciências (Microbiologia) pela Universidade Federal do Rio de Janeiro com estagio no GREB-Universite Laval, Quebec, Canada (1998). 


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How to Cite

Steiner-Oliveira C, Maciel FA, Rodrigues LK, Napimoga MH, Pimenta LA, Höfling JF, Gonçalves RB. An in vitro microbial model for producing caries-like lesions on enamel. Braz. J. Oral Sci. [Internet]. 2016 Jan. 21 [cited 2022 Nov. 28];6(22):1392-6. Available from:




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