Effects of Coffea arabica on Streptococcus mutans adherence to dental enamel and dentine





Streptococcus mutans. Coffea arabica. Dental enamel


The aim of this study was to evaluate the effects of different coffee solutions on Streptococcus mutans adherence to human dental enamel and dentine. Seventy-five specimens of human enamel and 75 of dentine were included in the study. Coffee solutions were prepared with two different trade marks of coffee (Mellita® and Pilão®) and two methods of preparation. The specimens were divided into ten experimental groups (n=15) according to the coffee solution tested. Each specimen was transferred to cell culture plates containing offee solution and culture medium and 0.1 ml of Streptococcus mutans standardized suspension was inoculated into each well. After the period of incubation, the number of bacterial cells adhered to each specimen was obtained by plating method. Results were analyzed by ANOVA and Tukey’s test (5%). Results showed that Streptococcus mutans adherence was statistically lower in the presence of the solution obtained by boiling method and the trade mark Pilão® (p=0.00) when compared with the other groups. All the test-groups presented values of cfu/ml significantly lower in relation to the control groups (p=0.00). It could be concluded, within the conditions of the study, that the coffee solutions tested reduced significantly the adherence of Streptococcus to dental enamel and dentine


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Author Biographies

Luciane Dias Oliveira, São Paulo State University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP (1998), Mestrado em Biopatologia Bucal (2002) UNESP, Doutorado em Biopatologia Bucal (2005) pela UNESP e Pós-Doutorado em Biopatologia Bucal (2009) pela UNESP, todos com bolsa FAPESP. É Professora Assistente Doutora das Disciplinas Farmacologia e Terapêutica Clínica do Curso de Odontologia do Instituto de Ciência e Tecnologia UNESP - São José dos Campos. 

Eloiza Helena da Silva Brandão, São Paulo State University

Possui graduação em odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho(2004). Tem experiência na área de Odontologia.

Cristiane Yumi Koga-Ito, São Paulo State University

Possui graduação em Odontologia pela UNESP, S. Jose dos Campos (1993), mestrado (1995) e doutorado em Biologia Patologia Buco-Dental pela UNICAMP (1997). Realizou pós-doutorado na Universidade de Turim- Itália (1997-1999) na área de Micologia Médica e na Universidade Federal de Minas Gerais na área de Micologia e Biologia Molecular (2000-2002).

Antonio Olavo Cardoso Jorge, São Paulo State University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1977), Especialização em Biologia e em Endodontia, Mestrado em Biologia (Fisiopatologia Celular) pela Universidade de Taubaté (1986), mestrado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1988), Doutorado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1991) e Livre-Docência em Microbiologia e Imunologia pela Faculdade de Odontologia de São José dos Campos/UNESP (1995).


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How to Cite

Oliveira LD, Brandão EH da S, Landucci LF, Koga-Ito CY, Jorge AOC. Effects of Coffea arabica on Streptococcus mutans adherence to dental enamel and dentine. Braz. J. Oral Sci. [Internet]. 2016 Jan. 22 [cited 2022 Nov. 30];6(23):1438-41. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643008




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