Effects of Coffea arabica on Streptococcus mutans adherence to dental enamel and dentine

Authors

DOI:

https://doi.org/10.20396/bjos.v6i23.8643008

Keywords:

Streptococcus mutans. Coffea arabica. Dental enamel

Abstract

The aim of this study was to evaluate the effects of different coffee solutions on Streptococcus mutans adherence to human dental enamel and dentine. Seventy-five specimens of human enamel and 75 of dentine were included in the study. Coffee solutions were prepared with two different trade marks of coffee (Mellita® and Pilão®) and two methods of preparation. The specimens were divided into ten experimental groups (n=15) according to the coffee solution tested. Each specimen was transferred to cell culture plates containing offee solution and culture medium and 0.1 ml of Streptococcus mutans standardized suspension was inoculated into each well. After the period of incubation, the number of bacterial cells adhered to each specimen was obtained by plating method. Results were analyzed by ANOVA and Tukey’s test (5%). Results showed that Streptococcus mutans adherence was statistically lower in the presence of the solution obtained by boiling method and the trade mark Pilão® (p=0.00) when compared with the other groups. All the test-groups presented values of cfu/ml significantly lower in relation to the control groups (p=0.00). It could be concluded, within the conditions of the study, that the coffee solutions tested reduced significantly the adherence of Streptococcus to dental enamel and dentine

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Luciane Dias Oliveira, São Paulo State University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP (1998), Mestrado em Biopatologia Bucal (2002) UNESP, Doutorado em Biopatologia Bucal (2005) pela UNESP e Pós-Doutorado em Biopatologia Bucal (2009) pela UNESP, todos com bolsa FAPESP. É Professora Assistente Doutora das Disciplinas Farmacologia e Terapêutica Clínica do Curso de Odontologia do Instituto de Ciência e Tecnologia UNESP - São José dos Campos. 

Eloiza Helena da Silva Brandão, São Paulo State University

Possui graduação em odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho(2004). Tem experiência na área de Odontologia.

Cristiane Yumi Koga-Ito, São Paulo State University

Possui graduação em Odontologia pela UNESP, S. Jose dos Campos (1993), mestrado (1995) e doutorado em Biologia Patologia Buco-Dental pela UNICAMP (1997). Realizou pós-doutorado na Universidade de Turim- Itália (1997-1999) na área de Micologia Médica e na Universidade Federal de Minas Gerais na área de Micologia e Biologia Molecular (2000-2002).

Antonio Olavo Cardoso Jorge, São Paulo State University

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1977), Especialização em Biologia e em Endodontia, Mestrado em Biologia (Fisiopatologia Celular) pela Universidade de Taubaté (1986), mestrado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1988), Doutorado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas (1991) e Livre-Docência em Microbiologia e Imunologia pela Faculdade de Odontologia de São José dos Campos/UNESP (1995).

References

Featherstone JDB. The science and practice of caries prevention. J Am Dent Assoc. 2000; 131: 887-99.

Daglia M, Papetti A, Dacarro C, Gazzani G. Isolation of antibacterial component from roasted coffee. J Pharm Bio An. 1998; 18: 219-25 3. Linke HA, Le Geros RZ. Black tea extract and dental caries formation in hamsters. Int J Food Sci Nutr. 2003; 54: 89-95.

Matsumoto M, Hamada S, Ooshima T. Molecular análisis of the inhibitory effects of oolong tea polyphenols on glucanbinding domain of recombinant glucosyltransferases from Streptococcus mutans MT8148. FEMS Microbiol Lett. 2003; 228: 73-80.

Matsumoto M, Minami T, Sasaki H, Sobue S, Hamada S, Ooshima T. Inhibitory effects of oolong tea extract on caries – inducing properties of Mutans streptococci. Caries Res. 1999; 33: 441-5.

Ooshima T, Osaka Y, Sasaki H, Osawa K, Yasuda H, Matsumura M, Sobue S, Matsumoto M. Caries inhibitory activity of cacao bean husk extract in in vitro and animal experiments. Arch Oral Biol. 2000; 45: 639-45.

Otake S, Makimura M, Kuroki T, Nishihara Y, Hirasawa M. Anticaries effects of polyphenolic compunds from japanese green tea. Caries Res. 1991; 25: 438-43.

Sasaki H, Matsumoto M, Tanaka T, Maeda M, Nakai M, Hamada S, Ooshima T. Antibacterial activity of polyphenol components in oolong tea extract against Streptococcus mutans. Caries Res. 2004; 38: 2-8.

Limsong J, Benjavongkulchai E, Kuvatanasuchati J. Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. J Ethnopharmacol. 2004; 92: 281-9.

Kashket S, Paolino VJ, Lewis DA, Van Houte J. In vitro inhibition of glucosyltransferase from the dental plaque bacterium Streptococcus mutans by commons beverages and food extracts. Arch Oral Biol. 1985; 30: 821-6.

Lima DR. Café e Saude: Manual de Farmacología Clínica, Terapêutica e Toxicologia. 3.ed. Rio de Janeiro: Medsi; 2003. p.141-9.

Toda M. The bactericidal activity of tea and coffe. Lett Appl Microbiol. 1989; 8: 123-5.

Kashket S, Paolino VJ, Lewis D, VanHoute J. Glucosyltransferase inhibition by tannin-like constituents of beverages. J Dent Res. 1985; 64: 212.

Landucci LF, Oliveira LD, Brandão EHS, Koga-Ito CY, GaettiJardim Jr E, Jorge AOC. Efeitos de Coffea arabica sobre a aderência de Streptococcus mutans à superfície de vidro. Cienc Odontol Bras. 2003; 6: 58-64.

Camargo MCR, Toledo MCF. Teor de cafeína em cafés brasileiros. Ciênc Tecnol Aliment. 1998; 18: 421-4.

Brandão EHS, Landucci LF, Oliveira LD, Koga-Ito CY, Jorge AOC. Antimicrobial activity of coffee-based solutions and their effect on Streptococcus mutans adherence. Braz J Oral Sci.

; 6: 1274-7.

Daglia M, Tarsi R, Papetti A, Grisoli P, Dacarro C, Pruzzo C et al. Antiadhesive effect of green and roasted coffee on Streptococcus mutans’ adhesive properties on saliva-coated hydroxyapatite beads. J Agric Food Chem. 2002; 50: 1225-9.

Paolino VJ, Kashket S, Sparagna CA. Inhibition of dextran synthesis by tannic acid. J Dent Res. 1980; 59: 389.

Osawa K, Miyazaki K, Shimura S, Okuda J, Matsumoto M, Ooshima T. Identification of cariostatic substances in the cacao bean husk: their anti-glucosyltransferase and antibacterial activities. J Dent Res. 2001; 80: 2000-4.

Downloads

Published

2016-01-22

How to Cite

1.
Oliveira LD, Brandão EH da S, Landucci LF, Koga-Ito CY, Jorge AOC. Effects of Coffea arabica on Streptococcus mutans adherence to dental enamel and dentine. Braz. J. Oral Sci. [Internet]. 2016 Jan. 22 [cited 2022 Nov. 30];6(23):1438-41. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643008

Issue

Section

Article

Most read articles by the same author(s)