Banner Portal
Efficacy of mouthrinse spray in inhibiting cariogenic biofilm formation on toothbrush bristles
Remote

Keywords

Chlorhexidine. Triclosan. Biofilm. Mutans streptococci. Toothbrush

How to Cite

1.
Nascimento AP, Faria G, Watanabe E, Ito IY. Efficacy of mouthrinse spray in inhibiting cariogenic biofilm formation on toothbrush bristles. Braz. J. Oral Sci. [Internet]. 2016 Sep. 23 [cited 2024 Dec. 13];7(24):1489-95. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643018

Abstract

Aim: The aim of this study was to evaluate the efficacy of Periogard® (Colgate-Palmolive) and Plax® (Colgate-Palmolive) in inhibiting biofilm formation of mutans streptococci (MS) on toothbrush bristles used just once for 2 minutes with no dentifrice. Methods: This study was performed into 3 phases in which mouthrinses and sterile distilled water (control group) were sprayed 6 times (to standardize the method) on toothbrush bristles used by 53 university students. Toothbrushes were kept at room temperature for 3 hours and put into test tubes each containing bacitracin sucrose broth (selective enrichment for MS). After incubation at 37ºC for 3 to 4 days, MS biofilm on the bristles were analyzed and colony-forming units (CFU) were counted by stereomicroscope under reflected light. Results: 41 students completed the 3 phases of this study and 38 (92.7%) control group toothbrushes had CFU of MS that varied from 1 to uncountable. Periogard® and Plax® inhibited biofilm formation on the bristles of 97.4% and 84.2% toothbrushes, respectively. Conclusion: Periogard® and Plax®, in spray, reduced/eliminated biofilm formation of MS on the toothbrush bristles. However, Periogard® was more efficacious than Plax® for this purpose.
https://doi.org/10.20396/bjos.v7i24.8643018
Remote

References

Glass RT, Lare MM. Toothbrush contamination: a potential health risk? Quintessence Int. 1986; 17: 39-42.

Cobb CM. Toothbrush as a cause of repeated infections of the mouth. Boston Med Surg J. 1920; 183: 263-4.

Svanberg M. Contamination of toothpaste and toothbrush by Streptococcus mutans. Scand J Dent Res. 1978; 86: 412-4.

Goldsmith RN, Shey Z, Houpt MI, Fine D, Schreiner H, Greenberg B. Toothbrush bristle wear and adherence of Streptococcus mutans. Pediatr Dent. 2007; 29: 243-7.

Bunetel L, Tricot-Doleux S, Agnani G, Bonnaure-Mallet M. In vitro evaluation of the retention of three species of pathogenic microorganisms by three different types of toothbrush. Oral Microbiol Immunol. 2000; 15: 313-6.

Nelson-Filho P, Macari S, Faria G, Assed S, Ito IY. Microbial contamination of toothbrushes and their decontamination. Pediatr Dent. 2000; 22: 381-4.

Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, Kozai K. Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children. J Med Microbiol. 2005; 54: 661-5.

Caudry SD, Klitorinos A, Chan ECS. Contaminated toothbrushes and their disinfection. J Can Dent Assoc. 1995; 61: 511-6.

Bhat SS, Hegde KS, George RM. Microbial contamination of tooth brushes and their decontamination. J Indian Soc Pedod Prev Dent. 2003; 21: 108-12.

Sato S, Pedrazzi V, Lara EHG, Panzeri H, Albuquerque Júnior RF, Ito IY. Antimicrobial spray for toothbrush disinfection: an in vivo evaluation. Quintessence Int. 2005; 36: 812-6.

Addy M. Chlorhexidine compared with other locally delivered antimicrobials: a short review. J Clin Periodontol. 1986; 13: 957-64.

Nabi N, Mukerjee C, Schmid R, Gaffar A. In vitro and in vivo studies on triclosan/PVM/MA copolymer/NaF combination as an anti-plaque agent. Am J Dent. 1989; 2: 197-206.

Jensen B, Bratthall D. A new method for the estimation of mutans streptococci in human saliva. J Dent Res. 1989; 68: 468-71.

Cesco RT, Bignelli P, Santos CP, Ito IY. Toothbrushes: evaluation of contamination level by streptococci of mutans group. In: 5th World Congress on Preventive Dentistry; São Paulo, 1995. São Paulo: Brazilian Association for Preventive Dentistry, 1995. p.103.

Quirynen M, De Soete M, Pauwels M, Gizani S, Van Meerbeek B, Van Steenberghe D. Can toothpaste or a toothbrush with antibacterial tufts prevent toothbrush contamination? J Periodontol. 2003; 74: 312-22.

Meier S, Collier C, Scaletta MG, Stephens J, Kimbrough R, Kettering JD. An in vitro investigation of the efficacy of CPC for use in toothbrush decontamination. J Dent Hyg. 1996; 70: 161-5.

Neal PR, Rippin JW. The efficacy of a toothbrush disinfectant spray – an in vitro study. J Dent. 2003; 31: 153-7.

The Brazilian Journal of Oral Sciences uses the Creative Commons license (CC), thus preserving the integrity of the articles in an open access environment.

Downloads

Download data is not yet available.