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Efficacy of mouthrinse spray in inhibiting cariogenic biofilm formation on toothbrush bristles


Chlorhexidine. Triclosan. Biofilm. Mutans streptococci. Toothbrush

How to Cite

Nascimento AP, Faria G, Watanabe E, Ito IY. Efficacy of mouthrinse spray in inhibiting cariogenic biofilm formation on toothbrush bristles. Braz. J. Oral Sci. [Internet]. 2016 Sep. 23 [cited 2024 Jun. 16];7(24):1489-95. Available from:


Aim: The aim of this study was to evaluate the efficacy of Periogard® (Colgate-Palmolive) and Plax® (Colgate-Palmolive) in inhibiting biofilm formation of mutans streptococci (MS) on toothbrush bristles used just once for 2 minutes with no dentifrice. Methods: This study was performed into 3 phases in which mouthrinses and sterile distilled water (control group) were sprayed 6 times (to standardize the method) on toothbrush bristles used by 53 university students. Toothbrushes were kept at room temperature for 3 hours and put into test tubes each containing bacitracin sucrose broth (selective enrichment for MS). After incubation at 37ºC for 3 to 4 days, MS biofilm on the bristles were analyzed and colony-forming units (CFU) were counted by stereomicroscope under reflected light. Results: 41 students completed the 3 phases of this study and 38 (92.7%) control group toothbrushes had CFU of MS that varied from 1 to uncountable. Periogard® and Plax® inhibited biofilm formation on the bristles of 97.4% and 84.2% toothbrushes, respectively. Conclusion: Periogard® and Plax®, in spray, reduced/eliminated biofilm formation of MS on the toothbrush bristles. However, Periogard® was more efficacious than Plax® for this purpose.


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