Cytotoxicity of intraoral orthodontic elastics

Authors

  • Rogério Lacerda Santos University of Rio de Janeiro
  • Matheus Melo Pithon University of Rio de Janeiro http://orcid.org/0000-0002-8418-4139
  • Márlio Vinícius Oliveira School of Pharmacy and Dentistry of Alfenas
  • Gabriella da Silva Mendes Federal University of Rio de Janeiro
  • Maria Teresa Villela Romanos Federal University of Rio de Janeiro
  • Antônio Carlos de Oliveira Ruellas Federal University of Rio de Janeiro

DOI:

https://doi.org/10.20396/bjos.v7i24.8643024

Keywords:

Cytotoxicity. Elastics. Biocompatibility. Orthodontics

Abstract

Aim: The present study compared in vitro four types of latex intraoral elastics (5/16 = 7.9 mm, mean load) regarding their possible cytotoxic effects. Material and Methods: The sample was allocated to four groups of 24 elastics: Group A (American Orthodontics), Group M (Morelli), Group T (TP Orthodontics), and Group U (Uniden). Cytotoxicity assays were performed using cell culture medium containing epithelioid-type cells (Hep-2 line) derived from human laryngeal carcinoma. Two methods for evaluating the cytotoxicity, the agar overlay test and “dye-uptake” test, were employed at two different moments (0 and 24 h). Data were compared by analysis of variance (ANOVA) and Tukey’s test (p<0.05). Results: The results showed statistically significant difference (p<0.05) between Groups T and M, T and U, A and M, and A and U, for dye-uptake test at 0 and 24 h. No cell lysis was found in Groups T and A for the agar overlay test, whereas 80% cell lysis was found in Groups M and U after 24 h according to the Stanford’s response index (RI). Conclusion: Both TP Orthodontics and American Orthodontics elastics evoked less cell lysis than Morelli and Uniden elastics

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Author Biographies

Matheus Melo Pithon, University of Rio de Janeiro

Especialista em Ortodontia pela Universidade Federal de Alfenas-Unifal, Mestre e Doutor em Ortodontia pela Universidade Federal do Rio de Janeiro-UFRJ e Pos-doutor em Odontopediatria pela Universidade Federal do Rio de Janeiro - UFRJ. Atualmente é Professor Adjunto da Disciplina de Ortodontia da Universidade Estadual do Sudoeste da Bahia (UESB).

Maria Teresa Villela Romanos, Federal University of Rio de Janeiro

Possui graduação em Ciências Biológicas pelo Instituto Superior de Ensino Celso Lisboa (1980), mestrado em Ciências (Microbiologia) pela Universidade Federal do Rio de Janeiro (1990) e doutorado em Ciências (Microbiologia) pela Universidade Federal do Rio de Janeiro (2001). Atualmente é professor adjunto da Universidade Federal do Rio de Janeiro. Tem experiência na área de Microbiologia, com ênfase em Virologia, atuando principalmente nos seguintes temas: antiviral, extratos de plantas, algas marinhas, hsv-1, hsv-2, vírus da dengue, adenovírus, vírus respiratórios e rotavírus. Experiência em citotoxicidade de biomateriais.

Antônio Carlos de Oliveira Ruellas, Federal University of Rio de Janeiro

Possui graduação em Odontologia pela Universidade Federal de Alfenas (1989), Mestrado em Odontologia pela Universidade Federal do Rio de Janeiro (1995) e Doutorado em Odontologia pela Universidade Federal do Rio de Janeiro (1999). 

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Published

2016-09-23

How to Cite

1.
Santos RL, Pithon MM, Oliveira MV, Mendes G da S, Romanos MTV, Ruellas AC de O. Cytotoxicity of intraoral orthodontic elastics. Braz. J. Oral Sci. [Internet]. 2016 Sep. 23 [cited 2022 Sep. 24];7(24):1520-5. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643024

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