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Influence of ultrasonic activation in association with different final irrigants on intracanal smear layer removal
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Root Canal Irrigants. Smear Layer. Ultrasonics.

How to Cite

Souza MA, Motter FT, Fontana TP, Ribeiro MB, Miyagaki DC, Cecchin D. Influence of ultrasonic activation in association with different final irrigants on intracanal smear layer removal. Braz. J. Oral Sci. [Internet]. 2016 Oct. 13 [cited 2023 Nov. 30];15(1):16-20. Available from:


Aim: To evaluate the influence of ultrasonic activation (US) with different irrigant regimens in smear layer removal. Methods: One hundred bovine incisors were instrumented and divided into ten groups (n=10) according to final irrigation protocols: distilled water (DW); DW+US; 17% EDTA; QMix; 10% citric acid; 37% phosphoric acid; 17% EDTA+US; QMix+US; 10% citric acid+US; 37% phosphoric acid+US. The samples were then submitted to scanning electron microscopy where a score system was used to evaluate the images and effectiveness of proposed treatments. The data were statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% significance level. Results: In the cervical third, groups 17% EDTA, QMix, 10% citric acid, 17% EDTA+US, QMix+US and 10% citric acid+US were more effective in smear layer removal (p<0.05); in the middle third, groups 17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05); in the apical third, groups 17% EDTA,17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05). Conclusions: US can aid 17% EDTA and QMix in smear layer removal at the middle third and QMix at the apical third, contributing to the cleaning of root canal system.
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Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol . 1965 Sep;20:340-9.

Teixeira CS, Felippe MC, Felippe WT. The effect of application time of EDTA and NaOCl on intracanal smear layer removal: an SEM analysis. Int Endod J. 2005 May;38(5):285-90.

Wang Z, Shen Y, Haapasalo M. Effect of smear layer against disinfection protocols on Enterococcus faecalis-infected dentin. J Endod. 2013 Nov;39(11):1395-400. doi: 10.1016/j.joen.2013.05.007.

Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F. Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod. 2007 Feb;33(2):96-105.

Pérez-Heredia M, Ferrer-Luque CM, González-Rodríguez MP, Martín-Peinado FJ, González-López S. Decalcifying effect of 15% EDTA, 15% citric acid, 5% phosphoric acid and 2.5% sodium hypochlorite on root canal dentine. Int Endod J. 2008 May;41(5):418-23. doi: 10.1111/j.1365-2591.2007.01371.x.

Stojicic S, Shen Y, Qian W, Johnson B, Haapasalo M. Antibacterial and smear layer removal ability of a novel irrigant, QMiX. Int Endod J. 2012 Apr;45(4):363-71. doi: 10.1111/j.1365-2591.2011.01985.x.

George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod. 2005 Dec;31(12):867-72.

van der Sluis LW, Versluis M, Wu MK, Wesselink PR. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J. 2007 Jun;40(6):415-26. Epub 2007 Apr 17.

Caron G, Nham K, Bronnec F, Machtou P. Effectiveness of different final irrigant activation protocols on smear layer removal in curved canals. J Endod. 2010 Aug;36(8):1361-6. doi: 10.1016/j.joen.2010.03.037.

Blank-Gonçalves LM, Nabeshima CK, Martins GH, Machado ME. Qualitative analysis of the removal of the smear layer in the apical third of curved roots: conventional irrigation versus activation systems. J Endod. 2011 Sep;37(9):1268-71. doi: 10.1016/j.joen.2011.06.009.

Prado M, Gusman H, Gomes BP, Simão RA. Scanning electron microscopic investigation of the effectiveness of phosphoric acid in smear layer removal when compared with EDTA and citric acid. J Endod. 2011 Feb;37(2):255-8. doi: 10.1016/j.joen.2010.11.011.

Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod. 2002 Jan;28(1):17-9.

Scelza MF, Pierro V, Scelza P, Pereira M. Effect of three different time periods of irrigation with EDTA-T, EDTA, and citric acid on smear layer removal. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Oct;98(4):499-503.

Plotino G, Pameijer CH, Grande NM, Somma F. Ultrasonics in endodontics: a review of the literature. J Endod. 2007 Feb;33(2):81-95.

Gründling GL, Zechin JG, Jardim WM, de Oliveira SD, de Figueiredo JA. Effect of ultrasonics on Enterococcus faecalis biofilm in a bovine tooth model. J Endod. 2011 Aug;37(8):1128-33. doi: 10.1016/j.joen.2011.05.006.

de Almeida AP, Souza MA, Miyagaki DC, Dal Bello Y, Cecchin D, Farina AP. Comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with Enterococcus faecalis: an in vitro study. J Endod. 2014 Dec;40(12):1953-7. doi: 10.1016/j.joen.2014.08.025.

Rödig T, Döllmann S, Konietschke F, Drebenstedt S, Hülsmann M. Effectiveness of different irrigant agitation techniques on debris and smear layer removal in curved root canals: a scanning electron microscopy study. J Endod. 2010 Dec;36(12):1983-7. doi: 10.1016/j.joen.2010.08.056.

Ribeiro EM, Silva-Sousa YT, Souza-Gabriel AE, Sousa-Neto MD, Lorencetti KT, Silva SR. Debris and smear removal in flattened root canals after use of different irrigant agitation protocols. Microsc Res Tech. 2012 Jun;75(6):781-90. doi: 10.1002/jemt.21125.

Jensen SA, Walker TL, Hutter JW, Nicoll BK. Comparison of the cleaning efficacy of passive sonic activation and passive ultrasonic activation after hand instrumentation in molar root canals. J Endod. 1999 Nov;25(11):735-8.

Saghiri MA, Delvarani A, Mehrvarzfar P, Malganji G, Lotfi M, Dadresanfar B, et al. A study of the relation between erosion and microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Dec;108(6):e29-34. doi: 10.1016/j.tripleo.2009.07.056.

Qian W, Shen Y, Haapasalo M. Quantitative analysis of the effect of irrigant solution sequences on dentin erosion. J Endod. 2011 Oct;37(10):1437-41. doi: 10.1016/j.joen.2011.06.005.

Aranda-Garcia AJ, Kuga MC, Chavéz-Andrade GM, Kalatzis-Sousa NG, Hungaro Duarte MA, Faria G, et al. Effect of final irrigation protocols on microhardness and erosion of root canal dentin. Microsc Res Tech. 2013 Oct;76(10):1079-83. doi: 10.1002/jemt.22268.

Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza-Filho FJ. In vitro assessment of the antimicrobial action and the mechanical ability of chlorhexidine gel as an endodontic irrigant. J Endod. 2001 Jul;27(7):452-5.

Böttcher DE, Sehnem NT, Montagner F, Fatturi Parolo CC, Grecca FS. Evaluation of the Effect of Enterococcus faecalis Biofilm on the 2% Chlorhexidine Substantivity: An In Vitro Study. J Endod. 2015 Aug;41(8):1364-70. doi: 10.1016/j.joen.2015.04.016.

Oliveira LD, Carvalho CA, Nunes W, Valera MC, Camargo CH, Jorge AO. Effects of chlorhexidine and sodium hypochlorite on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Oct;104(4):e125-8.

Giardino L, Ambu E, Becce C, Rimondini L, Morra M. Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic. J Endod. 2006 Nov;32(11):1091-3.

Niu LN, Luo XJ, Li GH, Bortoluzzi EA, Mao J, Chen JH, et al. Effects of different sonic activation protocols on debridement efficacy in teeth with single-rooted canals. J Dent. 2014 Aug;42(8):1001-9. doi: 10.1016/j.jdent.2014.05.007.

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