Salivary cortisol level and uncooperative behavior in pediatric dental practice

Authors

  • Ludmila Tavares Costa Ercolin Universidade Estadual de Campinas
  • Gláucia Maria Bovi Ambrosano Universidade Estadual de Campinas
  • Livia Fernandes Probst Universidade Estadual de Campinas
  • Margarete C. Ribeiro-Dasilva University of Florida, College of Dentistry, Department of Community Dentistry & Behavioral Science, Gainesville, FL, United States of America
  • Scott L. Tomar University of Florida
  • Rosana de Fátima Possobon Universidade Estadual de Campinas

DOI:

https://doi.org/10.20396/bjos.v15i1.8647125

Keywords:

Dental anxiety. Salivary elimination. Dental care.

Abstract

Aim: To investigate the relation between uncooperative behavior and salivary cortisol level in children who underwent preventive dental care. Methods: The sample was composed by 10 children of both sexes aged 40 to 52 months, presenting uncooperative behavior during dental preventive treatments. The saliva collection was performed using a cotton wheel and an Eppendorf tube (Sarstedt Salivete®) in 3 different moments: a) at home, on a day without dental treatment and at the same time on the day of the sessions treatment; b) 30 min after the end of the session, when there was manifestation of uncooperative behavior; c) 30 min after the end of the session, when there was a cooperative behavior of the child. A sample of saliva was centrifuged for 5 minutes at 2400 rpm, 1 of mL of saliva was pipetted in an Eppendorf tube and stored in a freezer at -20 ° C. For the determination of the levels of salivary cortisol was used an Active® kit for cortisol enzyme immunoassay (EIA) DSL-10-67100, composed of specific rabbit antibody anti-cortisol. Data were analyzed statistically for the uncooperative behavior issued in the beginning and at the end of sessions, using the paired t test (p<0.05) and for cortisol levels in saliva samples at home, after the beginning and at the end of sessions, using repeated-measures ANOVA and Tukey’s test (p<0.05). Results: During expression of uncooperative behavior in preventive dental care sessions the salivary cortisol level was significantly higher (0.65 ± 0.25 μg/dL) compared with expression of collaborative behavior (0.24 ± 0.10 μg/dL). Conclusions: It is possible to conclude that, even under preventive.

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Author Biographies

Ludmila Tavares Costa Ercolin, Universidade Estadual de Campinas

Possui graduação em Odontologia pela Faculdade de Odontologia de Piracicaba - Universidade Estadual de Campinas (2005). Mestrado em Odontologia, área de concentração Fisiologia Oral pela mesma instituição (2009). Doutorado em Odontologia, área de concentração Saúde Coletiva na FOP-UNICAMP (2014). Atua em Odontologia Preventiva nas áreas de odontopediatria da 1ª infância, manejo do comportamento infantil, odontologia para gestantes e incentivo ao aleitamento materno. Atua como pesquisadora da Síndrome de Burnout e qualidade de vida.

Gláucia Maria Bovi Ambrosano, Universidade Estadual de Campinas

Possui graduação em Engenharia Agronômica pela Universidade de São Paulo (1983), mestrado em Ciências pela Universidade de São Paulo (1987) e doutorado em Ciências pela Universidade de São Paulo (1994). Atualmente é professora titular da área de Bioestatística da Faculdade de Odontologia/UNICAMP. Tem experiência na área de Probabilidade e Estatística, com ênfase em Bioestatística, atuando principalmente nos seguintes temas: bioestatística na odontologia, amostragem, delineamentos experimentais, análise de dados.

Livia Fernandes Probst, Universidade Estadual de Campinas

Possui graduação em Odontologia pela Universidade Federal do Espírito Santo (2004), Mestrado em Odontologia em Saúde Coletiva pela Faculdade de Odontologia de Piracicaba-UNICAMP (2015), especialização em Saúde Coletiva pela UFES (2006) e em Atenção Primária à Saúde pela Faculdade Católica do Espírito Santo (2011). Atualmente é aluna de doutorado em Odontologia (Área Saúde Coletiva) da Faculdade de Odontologia de Piracicaba-UNICAMP e do curso de MBA em Economia e Avaliação de Tecnologias em Saúde (Proadi-SUS). Atua como professora e tutora dos cursos de Especialização em Saúde Coletiva de da Família (FOP-UNICAMP) e Gestão Pública em Saúde (FOP-UNICAMP). Tem experiência em temas interdisciplinares e atua principalmente nas áreas de Saúde Coletiva, Gestão em Saúde, Economia da Saúde e Avaliação de Tecnologias em Saúde.

Margarete C. Ribeiro-Dasilva, University of Florida, College of Dentistry, Department of Community Dentistry & Behavioral Science, Gainesville, FL, United States of America

Dr. Margarete C Ribeiro Dasilva é Especialista em Cirurgia Oral em Gainesville, Flórida. Graduada com honras em 2001. Tendo mais de 16 anos de diversas experiências, especialmente na CIRURGIA ORAL, a Dra. Margarete C Ribeiro Dasilva afilia-se a nenhum hospital e colabora com outros médicos e especialistas do grupo médico Faculty Associates Incorporated.

Scott L. Tomar, University of Florida

University of Florida, College of Dentistry, Department of Community Dentistry & Behavioral Science, Gainesville, FL, United States of America

Rosana de Fátima Possobon, Universidade Estadual de Campinas

Possui graduação em Odontologia pela Universidade Estadual de Campinas (1991), mestrado e doutorado em Odontologia pela Universidade Estadual de Campinas (2000 e 2003). Atualmente é Professora Associada da Àrea de Psicologia Aplicada da Faculdade de Odontologia de Piracicaba - Unicamp e coordenadora do Centro de Pesquisa e Atendimento Odontoógico para Pacientes Especiais - Cepae-FOP-Unicamp. Tem experiência na área de Odontologia, com ênfase em Psicologia Experimental, atuando principalmente nos seguintes temas: comportamentos de saúde, comportamento infantil, aleitamento materno e programa preventivo.

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Published

2016-10-13

How to Cite

1.
Ercolin LTC, Ambrosano GMB, Probst LF, Ribeiro-Dasilva MC, Tomar SL, Possobon R de F. Salivary cortisol level and uncooperative behavior in pediatric dental practice. Braz. J. Oral Sci. [Internet]. 2016 Oct. 13 [cited 2022 Aug. 12];15(1):57-61. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8647125

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