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Aim: To investigate the relation between uncooperative behavior and salivary cortisol level in children who underwent preventive dental care. Methods: The sample was composed by 10 children of both sexes aged 40 to 52 months, presenting uncooperative behavior during dental preventive treatments. The saliva collection was performed using a cotton wheel and an Eppendorf tube (Sarstedt Salivete®) in 3 different moments: a) at home, on a day without dental treatment and at the same time on the day of the sessions treatment; b) 30 min after the end of the session, when there was manifestation of uncooperative behavior; c) 30 min after the end of the session, when there was a cooperative behavior of the child. A sample of saliva was centrifuged for 5 minutes at 2400 rpm, 1 of mL of saliva was pipetted in an Eppendorf tube and stored in a freezer at -20 ° C. For the determination of the levels of salivary cortisol was used an Active® kit for cortisol enzyme immunoassay (EIA) DSL-10-67100, composed of specific rabbit antibody anti-cortisol. Data were analyzed statistically for the uncooperative behavior issued in the beginning and at the end of sessions, using the paired t test (p<0.05) and for cortisol levels in saliva samples at home, after the beginning and at the end of sessions, using repeated-measures ANOVA and Tukey’s test (p<0.05). Results: During expression of uncooperative behavior in preventive dental care sessions the salivary cortisol level was significantly higher (0.65 ± 0.25 μg/dL) compared with expression of collaborative behavior (0.24 ± 0.10 μg/dL). Conclusions: It is possible to conclude that, even under preventive.
References
Appukuttan DP. Strategies to manage patients with dental anxiety and dental phobia: literature review. Clin Cosmet Investig Dent. 2016 Mar 10;8:35-50. doi: 10.2147/CCIDE.S63626.
Milgrom P, Newton JT, Boyle C, Heaton LJ, Donaldson N. The effects of dental anxiety and irregular attendance on referral for dental treatment under sedation within the National Health Service in London. Community Dent Oral Epidemiol. 2010 Oct;38(5):453-9. doi: 10.1111/j.1600-0528.2010.00552.x.
Armfield JM, Heaton LJ. Management of fear and anxiety in the dental clinic: a review. Aust Dent J. 2013 Dec;58(4):390-407; quiz 531. doi: 10.1111/adj.12118.
Possobon RF, Caetano MES, Moraes ABA. [Dental treatment of uncooperative children: a case report]. Rev Bras Odontol. 1998;55(2):80-3. [Portuguese].
Possobon RF. [Effects of diazepam on no-collaborative behavior of children in dental care] [tese]. Piracicaba: Faculdade de Odontologia de Piracicaba/Unicamp; 2003. [Portuguese].
Wright GZ, Kupietzky A. Behavior management in dentistry for children. John Wiley & Sons; 2014.
Wilson S, Thikkurissy S, Gosnell ES. Behavior and the Child. In: Oral Sedation for Dental Procedures in Children. Springer Berlin Heidelberg. 2015. p.7-24.
Polk DE, Nolan BA, Shah NH, Weyant RJ. Policies and Procedures That Facilitate Implementation of Evidence-Based Clinical Guidelines in U.S. Dental Schools. J Dent Educ. 2016 Jan;80(1):23-9.
Jessop DS, Turner-Cobb JM. Measurement and meaning of salivary cortisol: A focus on health and disease in children. Stress, 2008 Jan;1(1)1-14. Epub 2007 Jul 16.
Blomqvist M, Holmberg K, Lindblad F, Fernell E, Ek U, Dahllof G. Salivary cortisol levels and dental anxiety in children with attention deficit hyperactivity disorder. Eur J Oral Sci 2007 Feb;115(1):1-6.
Selye H. A syndrome produced by diverse noccious agents. Nature. 1936 July 4;138(3479):32.
Quinkler M, Beuschlein F, Hahner S, Meyer G, Schöfl C, Stalla GK. Adrenal cortical insufficiency—a life threatening illness with multiple etiologies. Dtsch Arztebl Int. 2013 Dec 23;110(51-52):882-8. doi: 10.3238/arztebl.2013.0882.
Buchanan TW, Brechtel A, Sollerslll JJ, Lovallo WR. Exogenous cortisol exerts effects on the startle reflex independent of emotional modulation. Pharmacol Biochem Behav. 2001 Feb;68(2):203-10.
Petrakova L, Doering BK, Vits S, Engler H, Rief W, Schedlowski M, et al. Psychosocial Stress Increases Salivary Alpha-Amylase Activity Independently from Plasma Noradrenaline Levels. PLoS One. 2015 Aug 6;10(8):e0134561. doi: 10.1371/journal.pone.0134561.
Ponzi D, Muehlenbein MP, Sgoifo A, Geary DC, Mark V. Day-to-day Variation of Salivary Cortisol and Dehydroepiandrosterone (DHEA) in Children from a Rural Dominican Community Flinn. Adapt Human Behav Physiol. 2015 Mar;1(1):4-16.
Antonini SR, Jorge SM, Moreira AC. The emergence of salivary cortisol circadian rhythm and its relationship to sleep activity in preterm infants. Clin Endocrinol 2000 Apr;52(4):423-6.
Blomqvist M, Holmberg K, Lindblad F, Fernell E, Ek U, Dahllöf G. Salivary cortisol levels and dental anxiety in children with attention deficit hyperactivity disorder. Eur J Oral Sci. 2007 Feb;115(1):1-6.
Kandemir S, Oksan T, Alpoz AR, Ergezer Z, Kabalak T. Salivary cortisol levels in children during dental treatment. J Marmara Univ Dent Fac 1997 Sep;2(4):639-42.
Akyuz S, Pince S, Hekin N. Children’s stress during a restorative dental treatment: assessment using salivary cortisol measurements. J Clin Pediatr Dent 1996 Spring;20(3):219-23.
Lac G, Chamoux A. Elevated salivary cortisol levels as a result of sleep deprivation in a shift worker. Occup Med (Lond) 2003 Mar;53(2):143-5.
Gaab J, Huster D, Peisen R, Engert V, Heitz V, Schad T et al. Assessment of cortisol response with low-dose and high-dose ACTH in patients with chronic fatigue syndrome and healthy comparison subjects. Psychosomatics 2003 Mar-Apr;44(2):113-9.
Hill CM, Walker RV. Salivary cortisol determinations and self-rating scales in the assessment of stress in patients undergoing the extraction of wisdom teeth. Br Dent J 2001 Nov 10;191(9):513-5.
Kruerge TH, Heller HW, Hauffa BP, Haake P, Exton MS, Schedlowski M. The dental anxiety scale and effects of dental fear on salivary cortisol. Percept Mot Skills 2005 Feb;100(1):109-17.
Greabu M, Purice M, Totan A, Spinu T, Totan C. Salivary cortisol-marker of stress response to different dental treatment. Rom J Intern Med 2006;44(1):49-59.
Milgrom P, Fiset L, Melnick S, Weinstein P. The prevalence and practice management consequences of dental fear in a mayor U.S. City. J Am Dent Asoc. 1988 May;116(6):641-7.
Townend E, Dimigen G, Fung D. A clinical study of child dental anxiety. Behav Res Ther. 2000 Jan;38(1):31-46.
Possobon RF, Carrascoza KC, Moraes ABA, Costa Jr AL. [Dental treatment as a cause of anxiety]. Psicol Estud. 2007 Sep/Dec;12(3):609-16. [Portuguese].
Liddell A, Gosse V. Characteristics of early unpleasant dental experiences. J Behav Ther Exp Psychiatry. 1998 Sep;29(3):227-37.
Fioravante DP, Soares MRZ, Silveira JM, Zakir NSA. [The patient-professional relationship functional analysis in the pediatric dentistry. Estud Psicol. 2007 Apr/Jun;24(2):267-77. [Portuguese].
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