Aim: To assess the effect of adding zinc oxide nanoparticles to dental adhesives on their anti-microbial and bond strength properties. Methods: 45 human premolars were cut at the cement enamel junction (CEJ) and the crowns were sliced into buccal and lingual halves. The specimens were classified into three groups, etched with 37% phosphoric acid for 15 s and rinsed for 30 s. Single Bond, Single Bond+5% zinc oxide and Single Bond+10% zinc oxide were used in the first, second and third groups. A cylinder of Z250 composite was bonded and cured for 40 s. For anti-bacterial testing, 10 samples of each group were assessed by direct contact test; 10 μL of bacterial suspension was transferred into tubes containing adhesives and incubated for one hour; 300 μL of brain heart infusion (BHI) broth was added to each tube and after 12 h, 50 μL of bacteria and broth were spread on blood agar plates and incubated for 24 h. Results: The colony count decreased significantly in the second and third groups compared to the first. Conclusions: Incorporation of zinc oxide nanoparticles into dental adhesives increases their anti-microbial properties without affecting their bond strength.
Opdam NJ, Bronkhorst EM, Roeters JM, Loomans BA. A retrospective clinical study on longevity of posterior composite and amalgam restorations. Dent Mater. 2007 Jan;23(1):2-8.
Atali PY, Buuml F. The effect of different bleaching methods on the surface roughness and hardness of resin composites. J Dent Oral Hyg. 2011;3(2):10-7.
Melo MAS, Cheng L, Weir MD, Hsia RC, Rodrigues LK, Xu HH. Novel dental adhesive containing antibacterial agents and calcium phosphate nanoparticles. J Biomed Mater Res B Appl Biomater. 2013 May;101(4):620-9. doi: 10.1002/jbm.b.32864.
Sawai J. Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. J Microbiol Methods. 2003;54(2):177-82.
Dumont VC, Silva RM, Almeida-Júnior LE, Roa JPB, Botelho AM, Santos MH. Characterization and evaluation of bond strength of dental polymer systems modified with hydroxyapatite nanoparticles. J Mater Sci Chem Eng. 2013 Dec;1(7): 13-23. doi: 10.4236/msce.2013.17003.
Kasraei S, Sami L, Hendi S, AliKhani M-Y, Rezaei-Soufi L, Khamverdi Z. Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restor Dent Endod. 2014 May;39(2):109-14. doi: 10.5395/rde.2014.39.2.109.
Hirota K, Sugimoto M, Kato M, Tsukagoshi K, Tanigawa T, Sugimoto H. Preparation of zinc oxide ceramics with a sustainable antibacterial activity under dark conditions. Ceramics International. 2010 Mar;36(2):497-506. doi:10.1016/j.ceramint.2009.09.026.
Osorio R, Yamauti M, Osorio E, Ruiz-Requena M, Pashley DH, Tay F, et al. Zinc reduces collagen degradation in demineralized human dentin explants. J Dent. 2011 Feb;39(2):148-53. doi: 10.1016/j.jdent.2010.11.005.
Melo MAS, Cheng L, Zhang K, Weir MD, Rodrigues LK, Xu HH. Novel dental adhesives containing nanoparticles of silver and amorphous calcium phosphate. Dent Mater. 2013 Feb;29(2):199-210. doi: 10.1016/j.dental.2012.10.005.
Hendriksen RS, editor. Global Salm-Surv. A global Salmonella surveillance and laboratory support project of the World Health Organization. Laboratory Protocols. Level 2 Training Course. MIC susceptibility testing of Salmonella and Campylobacter. 4th ed. 2003.
Hojati ST, Alaghemand H, Hamze F, Babaki FA, Rajab-Nia R, Rezvani MB, et al. Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles. Dent Mater. 2013 May;29(5):495-505. doi: 10.1016/j.dental.2013.03.011.
Osorio R, Cabello I, Toledano M. Bioactivity of zinc-doped dental adhesives. J Dent. 2014 Apr;42(4):403-12. doi: 10.1016/j.jdent.2013.12.006.
Niu L, Fang M, Jiao K, Tang L, Xiao Y, Shen L, et al. Tetrapod-like zinc oxide whisker enhancement of resin composite. J Dent Res. 2010 Jul;89(7):746-50. doi: 10.1177/0022034510366682.
Aydin Sevinç B, Hanley L. Antibacterial activity of dental composites containing zinc oxide nanoparticles. J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):22-31. doi: 10.1002/jbm.b.31620.
Spencer CG, Campbell PM, Buschang PH, Cai J, Honeyman AL. Antimicrobial effects of zinc oxide in an orthodontic bonding agent. Angle Orthod. 2009 Mar;79(2):317-22. doi: 10.2319/011408-19.1.
Larsen KS, Auld DS. Characterization of an inhibitory metal binding site in carboxypeptidase A. Biochemistry. 1991 Mar 12;30(10):2613-8.
Osorio R, Yamauti M, Osorio E, Román JS, Toledano M. Zinc‐doped dentin adhesive for collagen protection at the hybrid layer. Eur J Oral Sci. 2011 Oct;119(5):401-10. doi: 10.1111/j.1600-0722.2011.00853.x.
Spero JM, DeVito B, Theodore L. Regulatory Chemicals Handbook. Boca Raton: CRC Press; 2000.
Powers JM, Sakaguchi RL. Craig’s restorative dental materials. Saint Louis: Mosby Elsevier; 2012.
Toledano M, Yamauti M, Ruiz-Requena ME, Osorio R. A ZnO-doped adhesive reduced collagen degradation favouring dentine remineralization. J Dent. 2012 Sep;40(9):756-65. doi: 10.1016/j.jdent.2012.05.007.
Phaechamud T, Mahadlek J, Aroonrerk N, Choopun C, Charoenteeraboon J. Antimicrobial activity of ZnO-doxycycline hyclate thermosensitive gel. ScienceAsia. 2012;38(1):64-74. doi: 10.2306/scienceasia1513-1874.2012.38.064.
The Brazilian Journal of Oral Sciences uses the Creative Commons license (CC), thus preserving the integrity of the articles in an open access environment.