Comparison of two different methods for detecting periodontal pathogenic bacteria

Authors

  • Telma Blanca Lombardo Bedran Universidade Nove de Julho
  • Guilherme José Pimentel Lopes de Oliveira Universidade Estadual Paulista
  • Luis Carlos Spolidorio Universidade Estadual Paulista
  • Joni Augusto Cirelli Universidade Estadual Paulista Júlio de Mesquita Filho
  • Denise Palomari Spolidorio Universidade Estadual Paulista

DOI:

https://doi.org/10.20396/bjos.v15i3.8649599

Keywords:

Periodontal diseases. Polymerase chain reaction. Bacteria.

Abstract

Aim: To perform a comparative analysis between two methods for detecting Porphyromonas gingivalis, Tannerella forsythia and Porphyromonas endodontalis in periodontal plaque samples. Methods: The study sample consisted of twenty systemically healthy patients showing generalized chronic periodontitis. The subgingival samples for microbiological analysis were collected before (baseline) and 60 days after a basic periodontal therapy from 30 non-adjacent affected sites (Probing Depth (PD): 5-7 mm, Clinical Attachment Loss (CAL) ≥ 5 mm, positive for Bleeding on Probing (BOP)). Microbiological analysis was performed by PCR and qPCR. To allow a comparative analysis between both methods, qPCR was divided in three different scores (score 2: presence of more than 100 bacteria; score 1: presence of 10-100 bacteria, and score 0: absence of bacteria), in accordance to DNA quantity, while for PCR two scores were assigned: presence or absence of bacteria. Results: qPCR demonstrated higher sensitivity in the detection of these pathogens compared with PCR when scores 1 and 2 were considered positive. However, when only score 2 was considered positive, PCR and qPCR showed better agreement. Conclusions: qPCR demonstrated higher sensitivity than conventional PCR for detection of low numbers of microorganisms and can be useful for the quantification of periodontopathogens.

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Author Biographies

Telma Blanca Lombardo Bedran, Universidade Nove de Julho

Possui graduação em Faculdade de Odontologia de Araraquara pela Universidade Estadual Paulista Júlio de Mesquita Filho (2006), mestrado em Periodontia pela Universidade Estadual Paulista Júio de Mesquita Filho - Araraquara (2010) e doutorado em odontologia pela Universidade Estadual Paulista Júio de Mesquita Filho - Araraquara (2014). Realizou doutorado sanduíche na Universidade Laval no período de 2012-2013. Atualmente é professora da disciplina de Periodontia no curso de Odontologia da Universidade Nove de Julho (UNINOVE).

Guilherme José Pimentel Lopes de Oliveira, Universidade Estadual Paulista

Especialista, mestre e doutor em Periodontia pela Faculdade de Odontologia de Araraquara ( FoAr-Unesp). Atualmente é pós-doutorando da mesma faculdade.Trabalha com as linhas de pesquisa de tratamento das doenças periodontais, regeneração óssea e periodontal, biomateriais e implantes osseointegrados

Luis Carlos Spolidorio, Universidade Estadual Paulista

Possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1985), mestrado em Biologia e Patologia Buco Dental pela Universidade Estadual de Campinas (1991) e doutorado em Biologia e Patologia Buco Dental pela mesma Univerdidade(1996). Possui Pós Doutorado pela Univerdidade de Calgary - Calgary - Canadá. Professor Titular da Disciplina de Patologia Bucal do Departamento de Fisiologia e Patologia da Faculdade de Odontologia de Araraquara/UNESP.

Joni Augusto Cirelli, Universidade Estadual Paulista Júlio de Mesquita Filho

possui graduação em Odontologia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1989), mestrado em Periodontia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1995), doutorado em Periodontia pela Universidade Estadual Paulista Júlio de Mesquita Filho (1998) e pós-doutorado pela University of Michigan (2005-2007). Atualmente é professor assistente doutor da Universidade Estadual Paulista Júlio de Mesquita Filho. 

Denise Palomari Spolidorio, Universidade Estadual Paulista

Professor do Departamento de Fisiologia e Patologia, UNESP.

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Published

2017-08-11

How to Cite

1.
Bedran TBL, Oliveira GJPL de, Spolidorio LC, Cirelli JA, Spolidorio DP. Comparison of two different methods for detecting periodontal pathogenic bacteria. Braz. J. Oral Sci. [Internet]. 2017 Aug. 11 [cited 2023 Jan. 30];15(3):166-72. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8649599

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