Microbial contamination of a University dental clinic in Brazil

  • Gisely Naura Venâncio Faculdade São Leopoldo Mandic
  • Victor Hugo Marques Coelho Universidade Nilton Lins
  • Thiago Fontanella Cestari Universidade Federal do Amazonas
  • Maxine Ennata Alves de Almeida Associação Brasileira de Cirurgiões Dentistas
  • Carolinie Batista Nobre da Cruz Universidade do Norte
Keywords: Students. Dental. Biosecurity. Exposure to biological agents.

Abstract

Pathogens of the oral cavity of a patient can be transferred to the dental office surfaces by direct contact, aerosol instruments and blood or saliva. The objective of this study was to investigate the microbiological contamination presents in the stands, chairs and spittoons in the University Nilton Lins dental clinics, in Manaus, Amazonas. Samples were collected with sterile swabs and seeded in different microbiological culture media for the isolation of microorganisms collected from each room. Then, assays were carried out for identification of strains isolated from each environment, such as: Gram stain, DNA purification, Amplification of 16s rRNA genes and sequencing. All these experiments were performed in the LBS / ILMD / FIOCRUZ. It was found 40 CFU / mL in the stands, 43 on the chairs and 47 in the spittoons and it was also possible to identify microorganisms like Klebsiella pneumoniae, Shigella sonnei and Staphylococcus aureus. The greatest number of CFUs was found in Clinic 3 and it was observed that the spittoon was the dental surface with the highest number of CFUs. Some of the bacterial species isolated are opportunists, suggesting that more severe biosecurity measures must be taken in order to prevent cross-infection.Pathogens of the oral cavity of a patient can be transferred to the dental office surfaces by direct contact, aerosol instruments and blood or saliva. The objective of this study was to investigate the microbiological contamination presents in the stands, chairs and spittoons in the University Nilton Lins dental clinics, in Manaus, Amazonas. Samples were collected with sterile swabs and seeded in different microbiological culture media for the isolation of microorganisms collected from each room. Then, assays were carried out for identification of strains isolated from each environment, such as: Gram stain, DNA purification, Amplification of 16s rRNA genes and sequencing. All these experiments were performed in the LBS / ILMD / FIOCRUZ. It was found 40 CFU / mL in the stands, 43 on the chairs and 47 in the spittoons and it was also possible to identify microorganisms like Klebsiella pneumoniae, Shigella sonnei and Staphylococcus aureus. The greatest number of CFUs was found in Clinic 3 and it was observed that the spittoon was the dental surface with the highest number of CFUs. Some of the bacterial species isolated are opportunists, suggesting that more severe biosecurity measures must be taken in order to prevent cross-infection.

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Author Biographies

Gisely Naura Venâncio, Faculdade São Leopoldo Mandic
Doutoranda em Ciências Odontológicas - Área de Concentração Clínicas Odontológicas pela Faculdade São Leopoldo Mandic - Campinas-SP./ Mestre em Odontologia pela Universidade Federal do Amazonas (2014)./ Especialista em Prótese Dentária pela Universidade do Estado do Amazonas (2011)./ Especialista em Saúde da Família pela Universidade Cândido Mendes (2008)./ Graduação em Odontologia pela Universidade Federal do Amazonas (2006).
Victor Hugo Marques Coelho, Universidade Nilton Lins
Possui graduação em Odontologia pela Universidade Nilton Lins. Tem experiência na área de Odontologia, com ênfase em Cirurgia e Traumatologia Buco-maxilo-facial,e de pesquisa nas áreas:Microbiologia e Biologia Molecular, principalmente nos seguintes temas: identificação bacteriana e PCR.
Thiago Fontanella Cestari, Universidade Federal do Amazonas

Possui ensino-medio-segundo-graupelo Colégio Martha Falcão(2009). Tem experiência na área de Odontologia.

Maxine Ennata Alves de Almeida, Associação Brasileira de Cirurgiões Dentistas
Possui ensino-fundamental-primeiro-graupela Escola Estadual Humberto de Alencar Castelo Branco (2008), ensino-medio-segundo-grau pela Escola Estadual Humberto de Alencar Castelo Branco (2011) e aperfeicoamento em Cirurgia Bucomaxilofacial pelo Assoaciação Brasileira de Cirurgiões Dentistas do Amazonas (2016).
Carolinie Batista Nobre da Cruz, Universidade do Norte
Possui Mestrado em Diversidade Biológica pela Universidade Federal do Amazonas (UFAM) com graduação em Licenciatura Plena em Ciências Biológicas (UFAM). Atualmente é professora da Universidade do Norte - UNINORTE/Laureate, ministra de: Fisiologia Humana, Patologia Geral, Microbiologia, Anatomia dos Sistemas, Morfologia Humana, Sistema Circulatório e Hematopoiético, Sistema Endócrino, Agressão e Defesa, entre outros, também atua como apoio a direção da Escola de Ciências da Saúde com o Programa de Qualidade Acadêmica. Tem experiência de pesquisa nas áreas: Microbiologia, Biologia Molecular e Bioquímica, com ênfase principalmente nos seguintes temas: diagnóstico molecular de bactérias e vírus, identificação bacteriana, construção de kit`s de multiplex PCR, caracterização de genes de virulência, construção de bibliotecas metagenômicas, seleção de microrganismos produtores de enzimas de interesse industrial e fitoterápicos da região amazônica com ação antimicrobiana. 

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Published
2017-09-20
How to Cite
Venâncio, G. N., Coelho, V. H. M., Cestari, T. F., Almeida, M. E. A. de, & Cruz, C. B. N. da. (2017). Microbial contamination of a University dental clinic in Brazil. Brazilian Journal of Oral Sciences, 15(4), 248-251. https://doi.org/10.20396/bjos.v15i4.8650030
Section
Article