Impact of the use of Ethanolic Extract of Propolis, Flavonoid and Non-Flavonoid Propolis for Direct Pulp Capping in Collagen Type I Density

Keywords: Propolis. Collagen type I. Inflammation. Dental pulp. Rat.

Abstract

Aim: To analysis collagen type I density on inflamed rat dental pulp after capping with propolis. Methods: Flavonoid and non-flavonoid substances were purified from propolis. Eighty male rats were divided into five groups, each group consisting of 16 rats. As a negative control (group I), rats were not conducted any treatment. A class I cavity was prepared on the occlusal surface of right maxillary first molar. Dental pulp was exposed and allowed in oral environment for 60 minutes, then dental pulp capping with ethanolic extract of propolis (group II), flavonoid propolis (group III), non-flavonoid propolis (group IV), or calcium hydroxide as positive control (group V). Rats were sacrificed at 6 hours, 2, 4 or 7 days, biopsy samples were obtained, stained and viewed by light microscope. Data was statistically analysis using Freidman and Kruskal-Wallis tests. Results: Except in group I, collagen type I density was increased in group II, III, and V with the longer of observation time periods. However, in group IV, collagen type I density increased only on day 7. No statistically significant differences of collagen type I density among the groups for each time period were found. Conclusions: Propolis and flavonoid propolis may increase collagen density on inflamed rat dental pulp.

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Author Biographies

Ardo Sabir, Hasanuddin University
DDS, ms at Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Latief Mooduto, Airlangga University
Department of Conservative Dentistry at Airlangga University, Surabaya. 
Cahyono Kaelan, Hasanuddin University
Medical doctor di Fac. of Medicine Univ Hasanuddin di Makassar dan Sekitarnya, Sulawesi Selatan, Indonesia.
Sherly Horax, Hasanuddin University
Médica dentista.

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Published
2017-09-20
How to Cite
Sabir, A., Mooduto, L., Kaelan, C., & Horax, S. (2017). Impact of the use of Ethanolic Extract of Propolis, Flavonoid and Non-Flavonoid Propolis for Direct Pulp Capping in Collagen Type I Density. Brazilian Journal of Oral Sciences, 15(4), 264-268. https://doi.org/10.20396/bjos.v15i4.8650037
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Article