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Impact of the use of Ethanolic Extract of Propolis, Flavonoid and Non-Flavonoid Propolis for Direct Pulp Capping in Collagen Type I Density


Propolis. Collagen type I. Inflammation. Dental pulp. Rat.

How to Cite

Sabir A, Mooduto L, Kaelan C, Horax S. Impact of the use of Ethanolic Extract of Propolis, Flavonoid and Non-Flavonoid Propolis for Direct Pulp Capping in Collagen Type I Density. Braz. J. Oral Sci. [Internet]. 2017 Sep. 20 [cited 2024 Apr. 18];15(4):264-8. Available from:


Aim: To analysis collagen type I density on inflamed rat dental pulp after capping with propolis. Methods: Flavonoid and non-flavonoid substances were purified from propolis. Eighty male rats were divided into five groups, each group consisting of 16 rats. As a negative control (group I), rats were not conducted any treatment. A class I cavity was prepared on the occlusal surface of right maxillary first molar. Dental pulp was exposed and allowed in oral environment for 60 minutes, then dental pulp capping with ethanolic extract of propolis (group II), flavonoid propolis (group III), non-flavonoid propolis (group IV), or calcium hydroxide as positive control (group V). Rats were sacrificed at 6 hours, 2, 4 or 7 days, biopsy samples were obtained, stained and viewed by light microscope. Data was statistically analysis using Freidman and Kruskal-Wallis tests. Results: Except in group I, collagen type I density was increased in group II, III, and V with the longer of observation time periods. However, in group IV, collagen type I density increased only on day 7. No statistically significant differences of collagen type I density among the groups for each time period were found. Conclusions: Propolis and flavonoid propolis may increase collagen density on inflamed rat dental pulp.


Kuropatnicki AK, Szliszka E, Krol W. Historical aspects of propolis research in modern times. Evid Based Complement Alternat Med. 2013;2013:964149. doi: 10.1155/2013/964149.

Bogdanov S. Propolis: composition, health, medicine: a review. Bee Prod Sci. 2017; [cited 2017 Jun 2]. Available from: http://www.bee-hexagon. net/files/file/fileE/Health/PropolisBookReview.pdf.

Sforcin JM, Fernandes A Jr, Lopes CA, Bankova V, Funari SR. Seasonal effect on Brazilian propolis antibacterial activity. J Ethnopharmacol. 2000 Nov;73(1-2):243-9.

Wang K, Zhang J, Ping S, Ma Q, Chen X, Xuan H, et al. Anti-inflammatory effects of ethanol extracts of Chinese propolis and buds from poplar (Populus x canadensis). J Ethnopharmacol. 2014 Aug 8;155(1):300-11. doi: 10.1016/j.jep.2014.05.037.

Sforcin JM. Propolis and the immune system: a review. J Ethnopharmacol. 2007 Aug 15;113(1):1-14.

Toreti VC, Sato HH, Pastore GM, Park YK. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evid Based Complement Alternat Med. 2013; 2013: 697390. doi:10.1155/2013/697390.

Huang S, Zhang CP, Wang K, Li GQ, Hu FL. Recent advances in the chemical composition of propolis. Molecules. 2014 Nov;19(12):19610-32. doi: 10.3390/molecules191219610.

Anauate Netto C, Marcucci MC, Paulino N, Anido-Anido A, Amore R, de Mendonça S, et al. Effects of typified propolis on mutans streptococci and lactobacilli: a randomized clinical trial. Braz Dent Sci. 2013 Apr;16(2):316.

Purra AR, Mushtaq M, Acharya SR, Saraswati V. A comparative evaluation of propolis and 5.0% potassium nitrate as a dentine desensitizer: A clinical study. J Indian Soc Periodontol. 2014 Jul;18(4):466-71. doi:10.4103/0972-124X.138695.

Bhardwaj A, Velmurugan N, Sumitha, Ballal S. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis) in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: An in vitro study. Indian J Dent Res. 2013 Jan-Feb;24(1):35-41.

Prabhakar AR, Karuna YM, Yavagal C, Deepak BM. Cavity disinfection in minimally invasive dentistry-comparative evaluation of Aloe vera and propolis: A randomized clinical trial. Contemp Clin Dent. 2015 Mar;6(Suppl 1):S24-31. doi:10.4103/0976-237X.152933.

Bazvand L, Aminozarbian MG, Farhad A, Noormohammadi H, Impact of the use of Ethanolic Extract of Propolis, Flavonoid and Non-Flavonoid Propolis for Direct Pulp Capping in Collagen Type I Density

Hasheminia SM, Mobasherizadeh S. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study. Dent Res J (Isfahan). 2014 Jul;11(4):469-74.

Parolia A, Kundabala M, Rao NN, Acharya SR, Agrawal P, Mohan M, et al. A comparative histological analysis of human pulp following direct pulp capping with Propolis, Mineral Trioxide Aggregate and Dycal. Aust Dent J. 2010 Mar;55(1):59-64. doi:10.1111/j.1834-7819.2009.01179.x

Mejàre IA, Axelsson S, Davidson T, Frisk F, Hakeberg M, Kvist T, et al. Diagnosis of the condition of the dental pulp: a systemic review. Int Endod J. 2012 Jul;45(7):597-613. doi: 10.1111/j.1365-2591.2012.02016.x.

Brett D. A review of Collagen and Collagen-based Wound Dressing. Wounds. 2008 Dec;20(12):347-56.

Rangaraj A, Harding K, Leaper D. Role of collagen in wound management. Wounds. 2011 Jun;23:347-56.

Al-Shaher A, Wallace J, Agarwal S, Bretz W, Baugh D. Effect of propolis on human fibroblasts from the pulp and periodontal ligament. J Endod. 2004 May;30(5):359-61.doi: 10.1097/00004770-200504000-00012.

Jahromi MZ, Ranjbarian P, Shiravi S. Cytotoxicity evaluation of Iranian propolis and calcium hydroxide on dental pulp fibroblasts. J Dent Res Dent Clin Dent Prospects. 2014 Summer;8(3):130-3. doi: 10.5681/ joddd.2014.024.

Sabir A, Mooduto L, Kaelan C, Horax S. Determination of flavonoid classes in Trigona sp propolis from Luwu Regency, South Sulawesi Province, Indonesia. In: Local Scientific Committee of 44th APIMONDIA International Apicultural Congress, editors. Scientific Program Abstracts of 44th APIMONDIA International Apicultural Congress. Daejeon: Published by Local Scientific Committee; 2015. p.457.

Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009 SepOct;37(5):1528-42.

Ramos AFN, Miranda JL. Propolis: a review of its anti-inflammatory and healing actions. J Venom Anim Toxins incl Trop Dis. 2007;13(4):697-710. doi: 10.1590/S1678-91992007000400002

Abdel-Wahed RE, El-Kammar MH, Korittum AS, Edrees IR. Surgical and histological evaluation of the effectiveness of propolis on wound healing. Alexandria J Vet Sci. 2013;39(1):52-63.

Bretz WA, Chiego DJ Jr, Marcucci MC, Cunha I, Custódio, Schneider LG. Preliminary report on the effects of propolis on wound healing in the dental pulp. Z Naturfosch C. 1998 Nov-Dec;53(11-12):1045-8.

Jain S, Rai R, Sharma V, Batra M. Propolis in Oral Health: A Natural Remedy. World J Pharm Sci. 2014 Jan;2(1):90-4.

Kandhare AD, Ghosh P, Bodhankar SL. Naringin, a flavanone glycoside, promotes angiogenesis and inhibits endothelial apoptosis through modulation of inflammatory and growth factor expression in diabetic foot ulcer in rats. Chem Biol Interact. 2014 Aug 5;219:101-12. doi: 10.1016/j. cbi.2014.05.012.

Havsteen BH. The biochemistry and medical significance of flavonoids. Pharmacol Ther. 2002 Nov-Dec;96(2-3):67-202.

Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999;65(4):33753.

Sabir A, Tabbu CR, Agustiono P, Sosroseno W. Histological analysis of rat dental pulp tissue capped with propolis. J Oral Sci. 2005 Sep;47(3):135-8.

Sabir A. [Antibacterial activity of flavonoid and non-flavonoid of propolis Trigona sp. toward the growth of Streptococcus mutans (in vitro)]. Jitekgi (Sci and Tech Dent J). 2010;7(1):37-42. Indonesian.

Nelson Filho P, Silva LA, Leonardo MR, Utrilla LS, Figueiredo F. Connective tissue responses to calcium hydroxide-based root canal medicaments. Int Endod J. 1999 Aug;32(4):303-11.

Vesna D, Elena K, Vesna L. Histological evaluation of odontoblast-like cells response after capping application of calcium hydroxide and hydroxilapatite in dog’s pulp. Acta Vet. 2007 Jan;57(5-6):573-84.

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