Isoenzyme genotyping and phylogenetic analysis of oxacillin-resistance Staphylococcus aureus isolates

Authors

  • Marcelo Fabiano Gomes Boriollo Universidade Estadual de Campinas
  • Manoel Francisco Rodrigues Netto Universidade Estadual de Campinas
  • Jeferson Júnior da Silva Dentista
  • Thaísla Andrielle da Silva Universidade José do Rosário Vellano
  • Maysa Eduarda de Castro Universidade José do Rosário Vellano
  • Júlio César Elias Centro Universitário Herminio Ometto de Araras
  • José Francisco Höfling Universidade Estadual de Campinas

DOI:

https://doi.org/10.20396/bjos.v16i0.8650497

Keywords:

Oxacillin-resistant staphylococcus aureus. Genetic variability. Propagation dynamics. MLEE. Clustering analysis.

Abstract

Aim: The propagation of S. aureus in hospital and dental environments is considered an important public health problem since resistant strains can cause serious infections in humans. The genetic variability of 99 oxacillin-resistant S. aureus isolates (ORSA) from the dental patients (oral cavity) and environments (air) was studied by isoenzyme genotyping. Methods: S. aureus isolates were studied using isoenzyme markers (alcohol dehydrogenase, sorbitol dehydrogenase, mannitol-1-phosphate dehydrogenase, malate dehydrogenase, glucose dehydrogenase, D-galactose dehydrogenase, glucose-6-phosphate dehydrogenase, catalase and /-esterase) and genetic (Nei’s statistics) and cluster analysis (UPGMA algorithm). Results: A highly frequent polyclonal pattern was observed in this population of ORSA isolates, suggesting various sources of contamination or microbial dispersion. Genetic relationship analysis showed a high degree of polymorphism between the strains, and it revealed three taxa (A, B and C) distantly genetically related (0.653dij1.432) and fifteen clusters (I to XV) moderately related (0.282dij0.653). These clusters harbored two or more highly related strains (0dij0.282), and the existence of microevolutionary processes in the population of ORSA. Conclusion: This research reinforces the hypothesis of the existence of several sources of contamination and/or dispersal of ORSA of clinical and epidemiologically importance, which could be associated with carriers (patients) and dental environmental (air).

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Author Biographies

Marcelo Fabiano Gomes Boriollo, Universidade Estadual de Campinas

Possui graduação em Licenciatura em Ciências e Habilitação em Biologia pela UNIARARAS (1997), Especialização em Genética e Biologia Molecular pela Universidade Federal de Alfenas - Unifal-MG (2016), Mestrado em Biologia e Patologia Buco-Dental pela Universidade Estadual de Campinas - FOP/UNICAMP (2000), Doutorado em Biologia Patologia Buco-Dental pela Universidade Estadual de Campinas - FOP/UNICAMP (2004) e Pós-Doutorado pela Universidade Estadual Paulista - FOAr/UNESP (2007) e Universidade Estadual de Campinas - FOP/UNICAMP (2012). 

Manoel Francisco Rodrigues Netto, Universidade Estadual de Campinas

‎Doutorado em Biologia Buco-Dental pela Faculdade de Odontologia de Piracicaba (FOP/UNICAMP)

Jeferson Júnior da Silva, Dentista

Dentista

Thaísla Andrielle da Silva, Universidade José do Rosário Vellano

Graduada em Biomedicina, na Universidade José do Rosário Vellano - UNIFENAS. Habilitada nas áreas de Microbiologia, Genética e Biologia Molecular. Possui mestrado na área de Ciência Animal na Universidade José do Rosário Vellano - UNIFENAS, com ênfase em farmacogenética. É especialista em Biologia Molecular e Genética pela Universidade Federal de Alfenas - UNIFAL. Atualmente é doutoranda na área de Reprodução e Bem-estar Animal na Universidade José do Rosário Vellano - UNIFENAS.

Maysa Eduarda de Castro, Universidade José do Rosário Vellano

Graduada em Biomedicina, na Universidade José do Rosário Vellano - UNIFENAS. Habilitada nas áreas de Análises clínicas e Imagenologia. Possui mestrado na área de Ciência Animal na Universidade José do Rosário Vellano - UNIFENAS/ Alfenas.Desenvolve pesquisas no Laboratório de Farmacogenética e Biologia Molecular da Universidade José do Rosário Vellano - UNIFENAS e é integrante do grupo de pesquisa "Ensaios Farmacológicos, Carcinogênicos, Mutagênicos e Teratogênicos de Produtos Terapêuticos.

Júlio César Elias, Centro Universitário Herminio Ometto de Araras

Possui graduação em Odontologia pelo Centro Universitário Herminio Ometto de Araras (1991) e Mestrado em Pesquisas Laboratoriais em Saúde Pública pelo Centro de Controle de Doenças Ses Gov Sp (2004). Doutorando em ATM pelo Centro de Controle de Doenças Ses Gov Sp Atualmente é Cirurgião Buco Maxilo Facial e ATM do Hospital Mediacl Center e do Sintra de Araras. Aluno de pós graduação do Centro de Controle de Doenças Ses Gov Sp e Cirurgião Buco Maxilo Facial do Centro de Tratamento e Diagnóstico de Atm Cirurgia Implantes. Tem experiência na área de Odontologia, com ênfase em Cirurgia Buco Maxilo Facial, atuando principalmente nos seguintes temas: ATM; Cirurgia, água, hemodiálise, pseudomonas aeruginosa, leveduras,água,equipo odontológico,pseudomonas aer, yeast, periodontic e leveduras, periodontia.

José Francisco Höfling, Universidade Estadual de Campinas

Atualmente é Professor Titular da Faculdade de Odontologia de Piracicaba, da Universidade Estadual de Campinas. Formou-se em Licenciatura e Bacharelado em Historia Natural pela Faculdade de Filosofia, Ciencias e Letras de Rio Claro, hoje UNESP "campus" de Rio Claro, em 1970. Desde 1967, quando ainda aluno, foi bolsista da Fapesp ( iniciaçao cientifica) durante todo o periodo de graduacão. Fez o curso de Imunologia do Instituto de Biologia da Unicamp, como bolsista de Aperfeiçoamento da Fapesp apresentando um c. vitae já com trabalhos publicados na area de Sistematica e Evolucão de Hymenopteros, familia Apidae.

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Published

2017-12-15

How to Cite

1.
Boriollo MFG, Rodrigues Netto MF, Silva JJ da, Silva TA da, Castro ME de, Elias JC, Höfling JF. Isoenzyme genotyping and phylogenetic analysis of oxacillin-resistance Staphylococcus aureus isolates. Braz. J. Oral Sci. [Internet]. 2017Dec.15 [cited 2021Dec.3];16:1-14. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8650497

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